User:Kristina M Holton/Ovarian Notebook 2007 9 14

Qiagen RNeasy FFPE Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)

• Step 5: Well-pelleted, no visible tumor sample in G03
• Step 7: White & cloudy
• Step 9: Better pelleted
• Step 11: Cloudy, using larger volumes
• Step 12: After 80C, clarified
• Step 15: 1750 EtOH for G02 as per Supplemental Protocol (too much for tube), 1400 EtOH for G03, Drapkin (fits into tube, original protocol calls for 1300ul), Small amount of precipitate
• Step 16: G02 requires 4 passes through column
• Step 21: Eluted in 30ul

QF 9/14/07	ng/ul	260/280	260/230	Vol	Total	Dilution	Total to Illumina
G02	674.89	1.95	1.69	60ul	40ug	30ul + 172ul H20	202ul
G03	879.55	2.02	2.06	60ul	52ug	30ul + 234ul H20	264ul
Drapkin	184.01	1.95	1.76	60ul	11ug	30ul + 25ul H20	55ul