User:Kristina M Holton/Ovarian Notebook 2007 7 6

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miRNA Extraction and Purification

Patient: PB

  • Used 3ml QIAzol
  • First centrifugation step needed (step 9)
  • Supernatant for tube 1->yellowish after 2nd centrifugation
  • After MiniPrep tube 1 looked fine-pooled both tubes
  • DNA yield: ~34ug