User:Kristina M Holton/Ovarian Notebook 2007 7 13

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Ambion RecoverAll Kit

  • Duodenum pellet - still small
  • Kidney pellet dropped in waste, then washed with EtOH and spun down again
  • Duodenum: slightly clear w/ precipitate; Kidney and Liver very yellow with a lot of precipitate: manual says,
   "Heavily oxidized & resistant to protease digestion -> slightly lower yields and smaller RNA fragments."
  • Kidney and Liver: still yellow after EtOH, filters more yellow
  • Yield: K:130.29, L:30.49, D:145.59 in 60ul

Qiagen easier