User:Kristina M Holton/Ovarian Notebook 2007 7 11

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Qiagen FFPE Kit Three samples -> duodenum, kidney, liver

  • Duodenum->small pellet, not as turbid as kidney and liver when adding to gDNA column
  • Used the higher amount of the two
  • Protocol changed at step 15 -> add 1120/1750 EtOH to flowthrough from step 14
  • Elute with 30ul RNase-free water
  • Duodenum and liver yield good (~300ng/ul), kidney not so much
 Homogenizer being sent back