User:Kristina M Holton/Ovarian Notebook 2007 9 25
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Total RNA Extraction Protocol: SW480 & SW620 cell lines Day 2
BioAnalyzer: NanoChip on cell line samples
Qiagen RNeasy FFPE kit: 12 samples
Notes on Protocol:
- Step 6: Samples 9,11,12 not well pelleted; 3 is red; 9 is brown; 11 small pellet
- Step 9: Sample 12 very poorly pelleted
- Step 14: Sample 7; Sample 10 poorly digested
| Sample | Sample ID | ng/ul | 260/280 | 260/230 | Vol | Total | Dilution | Total to Illumina |
| 1 | 502-35932-D | 278.11 | 1.94 | 1.69 | 30ul | 8.3ug | 15ul + 26.7ul H20 | 4.15ug |
| 2 | 502-35973-G | 191.71 | 2.0 | 1.77 | 30ul | 5.8ug | 15ul + 13.7ul H20 | 2.9ug |
| 3 | 502-36090-J | 150.43 | 1.99 | 1.84 | 30ul | 4.5ug | 15ul + 7.5ul H20 | 2.25ug |
| 4 | 502-36200-M | 1708.2 | 2.02 | 2.08 | 30ul | 51ug | 15ul + 241ul H20 | 25.5ug |
| 5 | 502-36433-AA | 1261.3 | 1.97 | 1.71 | 30ul | 38ug | 15ul + 174ul H20 | 19ug |
| 6 | 502-36752-E | 732.33 | 1.93 | 1.92 | 30ul | 22ug | 15ul + 95ul H20 | 11ug |
| 7 | 502-36839-I | 344.33 | 2.02 | 1.95 | 30ul | 10ug | 15ul + 37ul H20 | 5ug |
| 8 | 502-36878-B | 1612.9 | 2.02 | 2.10 | 30ul | 48ug | 15ul + 226ul H20 | 24ug |
| 9 | 502-36925-B | 529.03 | 1.97 | 2.04 | 30ul | 16ug | 15ul + 64ul H20 | 8ug |
| 10 | 502-37203-AE | 120.2 | 1.94 | 1.97 | 30ul | 3.6ug | 15ul + 3ul H20 | 1.8ug |
| 11 | 599-35521-E | 270.36 | 1.80 | 1.19 | 30ul | 8.1ug | 15ul + 26ul H20 | 4.05ug |
| 12 | 599-35521-N | 939.11 | 2.0 | 1.78 | 30ul | 28ug | 15ul + 126ul H20 | 14ug |
