User:Kristina M Holton/Ovarian Notebook 2007 7 17
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Bioanalyzer: 4 Ovarian Tissue Samples
- Diluted 1:10
- Used Nano chip -> gel less viscous and easier to practice with
- No rRNA bands, but miRNA bands ???
Suggestion: maybe too dilute
- Used Micro chip + my pipettes -> good results
- Easier to dispense gel + samples without bubbles
RNA MiniPrep: SW620, SW480
Use cells frozen 9/10/07 from P21/P17
First Round: (1 plate in 1 column)
- SW480: OD 650, 65ug
- SW620: OD 220, 22ug
Second Round: (1 plate in 2 columns)
- SW480: OD 1132, 135ug
- SW620: OD 1602, 192ug
Split 1 plate into 2 columns