User:Kristina M Holton/Ovarian Notebook 2007 7 17

From OpenWetWare
Jump to: navigation, search

Bioanalyzer: 4 Ovarian Tissue Samples

  • Diluted 1:10
  • Used Nano chip -> gel less viscous and easier to practice with
  • No rRNA bands, but miRNA bands ???
 Suggestion: maybe too dilute
  • Used Micro chip + my pipettes -> good results
  • Easier to dispense gel + samples without bubbles

RNA MiniPrep: SW620, SW480

Use cells frozen 9/10/07 from P21/P17

First Round: (1 plate in 1 column)

  • SW480: OD 650, 65ug
  • SW620: OD 220, 22ug

Second Round: (1 plate in 2 columns)

  • SW480: OD 1132, 135ug
  • SW620: OD 1602, 192ug
 Split 1 plate into 2 columns