Richard Lab:protocols

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Dionex IC

Eluent Preparation
Standard Preparation

Dionex ASE


Anaerobic Chamber


YSI Analyzer

Quantification of glucose


Biomass Protocols

Silage Protocols
Biomass Compositional Analysis
NDF (Neutral Detergent Fiber) Determination
ADF (Acid Detergent Fiber) Determination
ADL (Acid Detergent Lignin) Determination
Dilute-Acid Pretreatment of Biomass
Enzymatic Hydrolysis of Biomass
Quantification of Glucose
Simultaneous Saccharification and Co-Fermentation (SSCF)
SSF Ethanol production
Acidification of C and N Samples using Tartic Acid

Enzymatic Screens and Assays

Ferulic Acid Esterase Protocols
Laccase Protocols
Xylanase Protocols
Beta-galactosidase (LacZ) Assay
Beta-galactosidase Screen
Beta-glucuronidase (GusA) Protocols
Cellulase assays

Molecular/Synthetic Biology

Amplified Insert Assembly
Bio-Brick Formats
PCR Protocols
Site Directed Mutagenesis
Plasmid Extraction
DNA Quantification
DNA Concentration
DNA Synthesis
Restriction Digestion
Partial Restriction Digest
Agarose Gel Electrophoresis
Polyacrylamide Gel Electrophoresis
Gel Extraction of DNA
Preparation of Shuttle-vectors for transformation
Transforming Lactococcus lactis
Making Electro-competent E. coli
Electro-transformation of E. coli
Electro-transformation of L. Plantarum
Chromosomal Integration

Manure Storage and Emissions Analysis

Measuring and Analyzing Gas-Flux from a Surface
Setting Up Biofilters in the Field.
Formatting Data from Data Logger
BOD, moisture and VS content

Compost Analysis

Chemical Oxygen Demand
Compost Pile Monitoring
CO2 Respiration
Manure and Compost Sampling Preparation Protocols
Acidification of compost with 0.5 N HCl
Free Air Space Testing Procedure

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