Quantification of glucose

Introduction

This method is used to quantify glucose using a YSI 2700 Select biochemistry analyzer. The maximum concentration that should be read is 3 grams per liter, so dilutions may need to be made before analysis.

Method

SET-UP

1. Rinse out Buffer container and fill to line with YSI 2357 Buffer solution
2. Hit [Menu} then {2-MeasParameter] then [2-CalMethod] then [1-One Station]
3. Hit [Enter] twice to return to Measurement Parameter Set up Screen
4. Hit [3-Black]
5. Make sure Current BLACK probe chem is set to NONE. If it isn't, hit [1-Next chemistry] until NONE appears and hit [ENTER] to save
6. Hit [4-White] and set Current WHITE probe chem to Dextrose
7. Hit [Enter] 4 times to return to Measurement Parameter Set up Screen
8. Hit [Menu] to return to home screen

RUN SAMPLES

1. Hit [Run] and wait for system to complete calibration
2. When Ready screen appears, hit [Sample]
3. Present sample to needle in Station S3 without touching needle to tube
4. Hit [Sample] and wait for needle to retract
5. Results will print out at the completion of run
6. To continue testing samples, hit [Sample] when Ready screen appears

• Note: After 5-7 runs, system will automatically calibrate.

Notes

• Glucose standard good for 30 days after opening
• replace tubing every 6 months
• Glucose membrane needs to be replaced every 21 days (Ethanol membrane is every 5 days)