Richard Lab:IC standards

Carbohydrates

Overview

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Materials

Supplies

• 200-mL volumetric flask
• Spatulas
• IC pipettor (1000 uL)
• 15 mL purple cap tubes
• 10 mL pipet
• IC vials

Chemicals

• Arabinose
• Glucose
• Galactose
• Xylose
• Fructose
• Sucrose
• Mannose

Procedure

1. Prepare a standard solution containing a mixture of 7 sugars, each at 100 mg/L: glucose, galactose, xylose, fructose, arabinose, sucrose, and mannose.
   1. Weigh out 20 mg (0.020 g) of sugar within 2 mg. Record weight in lab notebook.
   2. Using volumetric flask, bring up to volume of 200 mL with nanopure water.
   3. Invert to dissolve/mix.
2. Prepare dilutions in 15 mL purple cap tubes using the recipe below. Pipet all sugar amounts using 1000μL pipettor. Pipet all water amounts using 10 mL pipet.
3. Mix standard solutions by vortexing. Filter using 0.2μM nylon filter and 10 mL syringe.
4. Label 5 IC vials with concentration and date for each standard solution. Pipet 1000μL into each vial. Freeze to store.
5. Aliquot 10 mL of filtered 100 mg/L sugar solution into five 15 mL purple caps tubes. Filter using 0.2μM nylon filter. Label with concentration and date. Freeze to store.

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Organic Acids

Overview

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Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

• supply 1 (i.e. tubes of a certain size? spreaders?)
• reagent 1
• X μL reagent 2
  • component A (reagent 2 is made up of multiple components)
  • component B
• equipment 1
• equipment 2

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

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Alcohols

Overview

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Materials

Supplies

• 100-mL volumetric flask
• IC pipettors (1000 uL and 200 uL)
• 15 mL purple cap tubes
• 10 mL pipet
• IC vials

Chemicals

• HPLC grade ethanol, 200 proof (density = 0.789 g/mL)
• HPLC grade methanol (density = 0.792 g/mL)

Procedure

1. Prepare a standard solution containing a mixture of methanol and ethanol, each at 500 mg/L.
   1. Add nanopure water to 100mL volumetric flask, until it almost reaches line.
   2. Pipet 63μL of methanol and 63μL of ethanol into volumetric flask.
   3. Using nanopure water, bring final volume to 100mL.
   4. Invert to dissolve/mix.
2. Prepare dilutions in 15mL purple cap tubes using the recipe below. Pipet all alcohol amounts using 1000μL pipettor. Pipet all water amounts using 10mL pipet.
3. Mix standard solutions by vortexing. Filter using 0.2μM nylon filter and 10mL syringe.
4. Label 5 IC vials with concentration and date for each standard solution. Also include 'EM' on label to identify as ethanol/methanol standards. Pipet 1000μL into each vial. Freeze to store.
5. Aliquot 10mL of filtered 100 mg/L sugar solution into five 15 mL purple caps tubes. Filter using 0.2μM nylon filter. Label with concentration and date. Freeze to store.

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• Who has experience with this protocol?

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