Richard Lab:IC overview

Overview

• Ion chromatograph with 2 independent pump and column systems and shared autosampler
• Carbohydrate analysis using CarboPac PA20 columns with pulsed amperometry (Au working electrode)
• Alcohol and organic acid analysis using ICE-AS1 columns with pulsed amperometry (Pt working electrode) and UV detection

Component Specifications

• Chromeleon Chromatography Management Software v. 6.8
• AS autosampler
  • 100-1.5mL vial capacity
  • 250μL needle, 10μL injection loops
  • Diverter valve for sequential (independent) control of dual column/pump system
• DC detector compartment
  • 15-40°C temperature control
  • Houses 2 ea. injection valves, columns, and ED detectors
• ED electrochemical detector
  • Combination pH and Ag/AgCl reference electrodes
  • Disposable Au or Pt working electrodes
• UVD340U UV/Vis detector
  • Simultaneous detection at 4 wavelengths (200-595nm)
• DP dual pump
  • 2 gradient pumps with integrated vacuum degasser
  • PEEK pump heads and flow path
• Shodex-101 refractive index detector (RID)
  • Not currently in use

Column Specifications

• CarboPac PA20
  • 3 x 30mm guard column
  • 3 x 150mm analytical column
  • Borate trap
• ICE-AS1
  • 4 x 50mm guard column
  • 4 x 250mm analytical column
  • 9 x 250mm analytical column
• CarboPac MA1 (not currently in use)

Sample Preparation

• Possible sample neutralization or clean-up
• Filtration with 0.2μM nylon or PTFE filters
• Dilution to existing standard curve range
  • 1-40 mg/L sugars
  • 25-200 mg/L alcohols and organic acids

References

• General equipment info from Dionex
  • http://www.dionex.com/en-us/products/ion-chromatography/ic-rfic-systems/ics-3000/lp-72594.html
• Column info from Dionex
  • http://www.dionex.com/en-us/products/columns/bio/carbohydrate/carbopac-pa20/lp-73367.html
  • http://www.dionex.com/en-us/products/columns/ic-rfic/ion-exclusion/ionpac-ice-as1/lp-73268.html

Contact

• Who has experience with this protocol?

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