Richard Lab:ASE overview

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  • Automated extraction of solid samples with common solvents at elevated temperature and pressure
  • Extraction steps: preheating sample cell (optional), filling cell with solvent, heating (equilibration), static extraction, rinsing, purging solvent and extractives into collection vessel, and relief
  • Current applications
    • Water and 95% ethanol extractions for NREL biomass compositional analysis
    • Liquid hot water biomass pretreatment


  • Oven temperature
    • Ambient to 200°C
  • Pressure
    • ~1600 psi
  • Available sample cells
    • 13-10mL stainless steel (~1.5g dry biomass)
    • 6-66mL zirconium (~10g dry biomass)
    • 6-100mL zirconium
  • Available extract collection vessels
    • 60mL vials
    • 250mL bottles

Sample Preparation Considerations

  • Grinding
    • More surface area for efficient extraction
  • Sample moisture
    • Samples may require mixing with drying (diatomaceous earth) or dispersing (Ottawa sand) agent
  • Sample pH
    • Mildly acidic or basic samples can be extracted in zirconium cells




  • Who has experience with this protocol?

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