Richard Lab:PCR
From OpenWetWare
Jump to navigationJump to search
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
- For Amplified Insert Assembly
- With Taq Polymerase
- With Vent Polymerase
- With Phusion Polymerase
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis
Quantification
- mRNA Quantification by qPCR
- Population Analysis by qPCR
