BME100 f2014:Projects5

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BME 100 Fall 2014 Home
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Lab 5: “Fluorescence Detection of DNA”

  • Content from PCR Labs B, C, D
    • Procedure
    • Data Analysis
    • SNP information & primer design

HW: Lab Report 5 (due 11/11/14 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
15 points – Lab B/C content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter
15 points – Lab B/C content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary
10 points - Lab D content - SNP information and primer design
50 points – Peer Assessment


OVERALL LAYOUT OUR TEAM section 5 pts = Original & non-offensive images; real names 3 pts = More than half original images & information 1 pt = All default green & white avatars; default text
Grammar & style 5 pts = Composition & layout perfect throughout 3 pts = Few spelling errors; images too big or small 1 pt = Many errors
PROCEDURE Camera settings 5 pts = Type of smartphone; settings where applicable, or "N/A" 3 pt = Brand name only; vague 1 pts = Missing all info
Calibration & solutions used 5 pts = Clear description; distance btwn. camera & drop included; complete table in Wiki format 3 pts = Missing one part of the information; Screen capture (e.g., jpeg) instead of wiki table 2 pts = Missing two parts or poorly written
BONUS (5 points) - Photo of flourimeter set-up 5 pts = Clear, good size; labels or caption added 3 pts = Photo with no labels/ caption 1 pt = Photo is over-sized; unclear
Placing samples onto the fluorimeter 5 pts = Steps are thorough, clear, and easy to follow 3 pts = Run-on paragraphs 1 pt = Instructions are vague/ incomplete
DATA ANALYSIS Drop images, INTDEN value table, plot with best fit line 10 pts = Complete 7 pts = Mising one part; very short overall 4 pt = Missing two or more parts
PCR results summary 5 pts = 100% Complete 3 pts = More than 50% complete 1 pt = Less than 50% complete
SNP INFO. & PRIMER DESIGN Background 5 pts = Well-written full sentences. 3 pts = Unclear, short (< 5 sentences), mostly phrases/ fragments 1 pt = Unclear, extremely short
Primer design & testing 5 pts = Short summary & image of web tool results 3 pts = Missing summary 1 pt = Missing image or both

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points

Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.


Look for your Group's lab report link in the list of links on the right. If it is red instead of blue, do not click on it!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.

<createbox> preload=Template:ASUBME103_f2014_L5 prefix=BME100_f2014: width=25 buttonlabel=Create Page </createbox>

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