BME100 f2014:Group18 L5

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Owwnotebook icon.png BME 100 Fall 2014 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Norah Alkhamis
Name: Jesus Calderon
Name: Kevin Couch
Name: Jordan Kariniemi
Name: Scott Slade
Name: Rachel Tomlinson


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: iPhone 5s
    • Flash: Off
    • ISO setting: iOS 8.1
    • White Balance: HDR Auto
    • Exposure: HDR Auto
    • Saturation: HDR Auto
    • Contrast: HDR Auto


Calibration

  • Distance between the smart phone cradle and drop = 8 cm


Solutions Used for Calibration

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Placing Samples onto the Fluorimeter

  1. Between two rows of the glass slide, rough side of the slide facing up, place 80 nanoliters of SYBR GREEN 1 onto the slide
  2. On top of the SYBR GREEN 1 droplet, place 80 nanoliters of the specified DNA concentration or PCR product sample
  3. Align the droplet of the SYBR GREEN 1 and the sample to be even with the blue light
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples Positive:

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Negative:

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Image J Values for All Calibrator Samples


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Calibration curve
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PCR Results Summary

  • Our positive control PCR result was 89.99279167 μg/mL
  • Our negative control PCR result was -22.78795833 μg/mL

Observed results Description of image

  • Patient 60166 :

Replicant 1: -48.34116667 (negative) Replicant 2: -26.844625 (negative) Replicant 3: 40.14679167 (positive)

  • Patient 99376 :

Replicant 1: -108.733625 (negative) Replicant 2: 108.914333 (positive) Replicant 3: -31.005125 (negative)
Conclusions

  • Patient 60166 : Negative
  • Patient 99376 : Negative




SNP Information & Primer Design

Background: About the Disease SNP To begin, “SNP” stands for “single nucleotide polymorphism”. A nucleotide consists of a nucleoside linked to a phosphate group. They form the basic structural unit of nucleic acids such as DNA. When dealing with genetics, polymorphism is the presence of genetic variation within a population.Using these two definitions to understand SNP more, we learn that SNP is simply a variation in a single base pair in a DNA sequence. The species that SNP is found in is homo sapiens, or the human species. Its clinical significance is that it is pathogenic. Based on previous research and investigation, these SNPs are associated with congenital long QT syndromes (LQTSs), which are heart rhythm disorders that can potentially cause fast, chaotic heartbeats.The rapid heartbeats can trigger a sudden fainting spell or seizure, and can even cause death in some cases from beating erratically for so long.

Primer Design and Testing