Lab Write-Up 6
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Your Bayesian statistics work must be submitted via an online quiz at Blackboard. Do not post your answers in Lab Report 6. In order to access the quiz:
- Go to the BME100 Blackboard site
- Click Content (in the left menu), then the Quizzes folder, then Bayes Calculation Quiz for your section (AM or PM)
- You may attempt the quiz multiple times. The number of points you earn will be 10% of your individual Lab Report 6 score.
- You must complete the quiz by the Lab Report 6 due date. After that time, the quiz will be closed.
Wednesday, 9:00 AM
Due Wednesday, November 27, 9:00 AM.
DNA Amplification and Measurement Results
pos = positive; neg = negative (no amplification)
Team's Conclusion - based on the median μg/mL value of three replicates and compared to positive and negative control PCR reactions
Expected - the "real answers", based on how the pre-mixed primer/template samples were set up by the instructor. If the Team's Conclusion matches the Expected outcome, then the procedure worked.
Team
|
Patient ID
|
Measured (ug/mL)
|
Team's Conclusion
|
Expected
|
AM 1 |
67618 |
0.60 |
0.65 |
0.61 |
incomplete |
pos
|
|
78379 |
0.62 |
0.60 |
0.73 |
incomplete |
pos
|
AM 2 |
56366 |
5.54 |
4.79 |
5.49 |
pos |
pos
|
|
86361 |
4.59 |
3.91 |
3.42 |
pos |
pos
|
AM 3 |
73190 |
7.62 |
7.89 |
7.788 |
pos |
pos
|
|
43566 |
8.16 |
10.62 |
11.1 |
pos |
pos
|
AM 4 |
89941 |
0.17 |
2.27 |
1.6 |
pos |
pos
|
|
59749 |
2.91 |
2.29 |
1.92 |
pos |
pos
|
AM 5 |
32298 |
7.20 |
9.40 |
8.4 |
incomplete |
pos
|
|
26685 |
7.50 |
7.20 |
9.2 |
incomplete |
neg
|
AM 6 |
88350 |
4.74 |
5.10 |
4.26 |
pos |
pos
|
|
65583 |
0.36 |
0.06 |
0.12 |
neg |
neg
|
AM 7 |
10939 |
18.60 |
-0.24 |
11.124 |
pos |
pos
|
|
57695 |
-1.39 |
-1.11 |
-1.13404 |
neg |
neg
|
AM 8 |
10072 |
1.80 |
1.80 |
1.8 |
pos |
pos
|
|
83576 |
-0.12 |
-0.09 |
-0.05 |
neg |
neg
|
AM 9 |
46398 |
11.53 |
10.03 |
7.989 |
pos |
pos
|
|
73945 |
-1.84 |
-1.846 |
-1.583 |
neg |
neg
|
AM 10 |
51225 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
|
60779 |
incomplete |
incomplete |
incomplete |
incomplete |
pos
|
AM 11 |
26317 |
-24.60 |
-23.18 |
-22.28 |
neg |
neg
|
|
63107 |
338.55 |
319.41 |
338.65 |
pos |
pos
|
AM 12 |
39526 |
1.59 |
0.93 |
2.44 |
neg |
neg
|
|
31856 |
11.14 |
12.60 |
14.29 |
pos |
pos
|
AM 13 |
26734 |
0.33 |
-1.08 |
-0.27 |
neg |
neg
|
|
22707 |
1.46 |
2.37 |
-1.88 |
pos |
pos
|
AM 14 |
16578 |
3.90 |
3.86 |
3.74 |
neg |
neg
|
|
25045 |
10.37 |
12.31 |
13.115 |
pos |
pos
|
AM 15 |
78086 |
12.66 |
8.64 |
8.05 |
no conclusion |
neg
|
|
32445 |
7.47 |
9.41 |
7.53 |
no conclusion |
neg
|
AM 16 |
28375 |
12.66 |
12.68 |
13.14 |
no conclusion |
neg
|
|
23170 |
0.86 |
0.92 |
0.97 |
no conclusion |
neg
|
AM 17 |
80926 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
|
83588 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
AM 18 |
34902 |
0.45 |
0.426 |
0.47 |
neg |
neg
|
|
39905 |
0.67 |
0.34 |
0.45 |
neg |
neg
|
Wednesday, 12:00 PM
Due Wednesday, November 27, 12:00 PM
DNA Amplification and Measurement Results
pos = positive; neg = negative (no amplification)
Team's Conclusion - based on the median μg/mL value of three replicates and compared to positive and negative control PCR reactions
Expected - the "real answers", based on how the pre-mixed primer/template samples were set up by the instructor. If the Team's Conclusion matches the Expected outcome, then the procedure worked.
Team
|
Patient ID
|
Measured (ug/mL)
|
Team's Conclusion
|
Expected
|
PM 1 |
16946 |
2.36 |
-2.24 |
-4.22 |
neg |
pos
|
|
46296 |
34.49 |
75.12 |
64.7 |
pos |
pos
|
PM 2 |
43849 |
4.5284 |
8.1235 |
3.6237 |
pos |
pos
|
|
80853 |
7.4525 |
5.5804 |
9.739 |
neg |
pos
|
PM 3 |
77842 |
178.8 |
147.1 |
147.6 |
pos |
pos
|
|
26204 |
152.4 |
59.8 |
117.3 |
pos |
pos
|
PM 4 |
51917 |
incomplete |
incomplete |
incomplete |
incomplete |
pos
|
|
17539 |
incomplete |
incomplete |
incomplete |
incomplete |
pos
|
PM 5 |
40197 |
15.08 |
20.58 |
20.61 |
pos |
pos
|
|
90029 |
-8.77 |
-9.05 |
-11.15 |
neg |
neg
|
PM 6 |
47569 |
incomplete |
incomplete |
incomplete |
incomplete |
pos
|
|
67318 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
PM 7 |
92069 |
5.56 |
3.86 |
2.92 |
pos |
pos
|
|
27677 |
0.83 |
-0.27 |
-1.03 |
neg |
neg
|
PM 8 |
21489 |
15.24 |
20.76 |
20.28 |
pos |
pos
|
|
90434 |
4.08 |
7.08 |
4.44 |
neg |
neg
|
PM 9 |
90616 |
56.94 |
44.68 |
58.66 |
pos |
pos
|
|
91514 |
5.14 |
4.44 |
2.1 |
neg |
neg
|
PM 10 |
74963 |
-1.08 |
-0.62 |
-0.93 |
neg |
neg
|
|
97208 |
7.79 |
6.62 |
7.01 |
pos |
pos
|
PM 11 |
54764 |
17.6 |
7.05 |
9.5 |
neg |
neg
|
|
51843 |
14.43 |
13.47 |
18.54 |
pos |
pos
|
PM 12 |
40908 |
-4.04 |
-1.52 |
-1.7 |
neg |
neg
|
|
51602 |
72.92 |
52.02 |
34.09 |
pos |
pos
|
PM 13 |
31526 |
-2.592 |
-6.876 |
-3.156 |
neg |
neg
|
|
36055 |
45.96 |
45.36 |
45.12 |
pos |
pos
|
PM 14 |
30337 |
271.3 |
7 |
257.5 |
neg |
neg
|
|
57707 |
1276.18 |
5611.1 |
1452 |
pos |
pos
|
PM 15 |
29108 |
11.88 |
10.38 |
12.06 |
neg |
neg
|
|
|
9.36 |
8.58 |
7.26 |
neg |
neg
|
PM 16 |
41731 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
|
78042 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
PM 17 |
37638 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
|
55609 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
PM 18 |
54174 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
|
43184 |
incomplete |
incomplete |
incomplete |
incomplete |
neg
|
Scoring Rubric
Click to enlarge this image
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