BME100 f2013:W1200 Group7 L6
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LAB 6 WRITE-UP
What is TinkerCad and How we Used it:
Another implication for using the Tinkercad program is that it only is used to tweak physical features. If we wanted to change something internally of a PCR machine, it wouldn’t show in the 3D model at all.
Feature 1: Cancer SNP-Specific Primers
Background on the cancer-associated mutation
What is rs17879961?
Feature 2: Consumables Kit
As previously mentioned, one of the problems with the kit was that it was that it made the experiment very time-consuming to run. Pipetting was also very tedious. The tubes in the packaging are stacked in our new design, making it much easier to access the tubes, ultimately making the experiment easier. The tubes not being pre-labeled made things much more difficult. The redesigned tubes are now labeled with 1A, 2A, 3A, 4A, 1B, 2B, 3B, 4B, etc. for greater ease of experimentation. The tubes were also redesigned with an accordion-like structure, making them able to be folded in for a more compact form of storage. These packaging changes will make the experiments that the scientists run much easier.
Feature 3: PCR Machine Hardware
The PCR machine will be used to target DNA replication in our system.
The aspect of the PCR machine that we have decided to redesign is the software and the display errors. The external software and transmission causes communication errors and can alter the results. Placing the software as an internal component rather than an external one, should also fix the display errors as well and improve the reliability of the PCR machine. The first time we used the machine half of the replications failed and and it took over 3 hours for 12 replications when it should have been more than that. This should hopefully improve the reliability as well as the speed of receiving results.
Feature 4: Fluorimeter Hardware
When using the fluorimeter a few issues were noted. The first was when the stand holding the sample was moved the drop of solution on top slid along the glass surface. This could be fixed by increasing the sturdiness and adding wheels so it could be transported more smoothly. Also, to insure consistent distance for the camera, a stand would be connected at the optimal distance for the camera to sit.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
Calculation 3 was the probability of patients with cancer testing positive meaning a positive predictive value. The percent value for this calculation was small (close to 0) and has low specificity (probability of person without the disease will test negative). This tells us that the PCR diagnostic test resulted in some patients being positive for cancer but they are however non-cancerous patients and that this test is not good with testing cancer patients. Calculation 4 was the negative predictive value. This value was slightly above .5 and this was the probability that a patient without cancer will test negative. This had a somewhat sensitivity (probability that a person with the disease being tested will test positive) and would be a somewhat a good test for testing with patients without cancer.