Magni:Protocols: Difference between revisions
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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Plasmid construction = === 1 === === 2 === = Characterization = === 1 === === 2 === </div>) |
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<div style="padding: 10px; width: 720px; border: 5px solid #000000;"> | <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> | ||
= Growth media = | |||
*[[Magni:Protocols/LB|LB]] | |||
*[[Magni:Protocols/M9|M9]] | |||
*[[Magni:Protocols/ph|pH adjustment for LB or M9]] | |||
*[[Magni:Protocols/buffering|Buffered media]] | |||
*[[Magni:Protocols/staining|Blue-white screening on plates]] | |||
= Plasmid construction = | = Plasmid construction = | ||
*[[Magni:Protocols/Competentcells|Competent cells]] | |||
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]] | |||
*[[Magni:Protocols/Transformation|Transformation]] | |||
*[[Magni:Protocols/Inocula|Plasmid propagation]] | |||
*[[Magni:Protocols/Miniprep|Miniprep]] | |||
*[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]] | |||
*[[Magni:Protocols/Electrophoresis|Electrophoresis]] | |||
*[[Magni:Protocols/gelextraction|Gel extraction]] | |||
*[[Magni:Protocols/purificationkit|DNA purification (with columns)]] | |||
*[[Magni:Protocols/sodiumacetate|DNA purification via ethanol precipitation with sodium acetate]] | |||
*[[Magni:Protocols/Ligation|Ligation]] | |||
*[[Magni:Protocols/PCR|PCR]] | |||
*[[Magni:Protocols/phosphatase|DNA dephosphorylation with Antarctic Phosphatase]] | |||
= Characterization = | = Characterization = | ||
*[[Magni:Protocols/tecan|Assays with Tecan Infinite F200 microplate reader]] | |||
*[[Magni:Protocols/fermentation|Fermentation]] | |||
*[[Magni:Protocols/chemostat|Chemostat]] | |||
*[[Magni:Protocols/flowcy|Preparation of samples for flow cytometry]] | |||
*[[Magni:Protocols/hplc|Preparation of samples for HPLC]] | |||
</div> | </div> | ||
Latest revision as of 23:49, 14 July 2013
Growth media
Plasmid construction
- Competent cells
- Plasmid resuspension from iGEM kit plates
- Transformation
- Plasmid propagation
- Miniprep
- BioBrick digestion with restriction enzymes
- Electrophoresis
- Gel extraction
- DNA purification (with columns)
- DNA purification via ethanol precipitation with sodium acetate
- Ligation
- PCR
- DNA dephosphorylation with Antarctic Phosphatase
