Magni:Protocols/staining

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Blue-white screening

This screening is to select colonies expressing a functional Beta-galactosidase protein (lacZ gene). They appear as blue colonies on agar plates.

• Pre-warm an LB agar plate at 37degC.
• For each plate, mix 20 ul of X-Gal (40 mg/ml dissolved in DMF), 20 ul of IPTG (200 mM, ReadyMade stock) and 160 ul of sterile LB.
• Spread evenly on the plate near the flame.
• Incubate it at 37degC until dry and use.

SAFETY NOTE: DMF is toxic, always use gloves and a face mask.