Magni:Protocols

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Growth media

• LB
• M9
• pH adjustment for LB or M9
• Buffered media
• Blue-white screening on plates

Plasmid construction

• Competent cells
• Plasmid resuspension from iGEM kit plates
• Transformation
• Plasmid propagation
• Miniprep
• BioBrick digestion with restriction enzymes
• Electrophoresis
• Gel extraction
• DNA purification (with columns)
• DNA purification via ethanol precipitation with sodium acetate
• Ligation
• PCR
• DNA dephosphorylation with Antarctic Phosphatase

Characterization

• Assays with Tecan Infinite F200 microplate reader
• Fermentation
• Chemostat
• Preparation of samples for flow cytometry
• Preparation of samples for HPLC