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Plasmid resuspension from iGEM kit plates

  • Check on the web site the kit plate and the exact coordinate of the desired plasmid.
  • Take the kit plates, stored in the -20degC freezer. They contain dry DNA with a red dye.
  • Take 15 ul of Milli-Q water (from the filter of the Millipore machine) with a P20 pipetman.
  • Make a hole in the foil covering the well by using the tip and resuspend the dry DNA several times. The DNA solution should become red.
  • Transfer the DNA into a 0.5-ml tube and transform 2-5 ul into competent cells.
  • Store the remaining DNA at -20degC.