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Preparation of samples for HPLC
- Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C.
- The storage of two samples is recommended for backup reasons.
- To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler.
- At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample in an "insert" inside a vial.