Magni:Protocols/Miniprep

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Miniprep

• Use the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the following exceptions:
  • saturated bacterial culture can be centrifuged at 4000 rpm, 4degC, 10 min;
  • elution step can be done with 30 ul of Milli-Q water (from the filter of the Millipore machine).