Magni:Protocols/LB

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LB medium (liquid)

• For every 1 L of medium, add to a pyrex bottle or flask:
  • 10 g NaCl
  • 10 g bacto-tryptone
  • 5 g yeast extract
• Add 1 L of deionized water (blue tap of the Millipore machine).
• Autoclave (if using a bottle, do not close the cap completely).
• Let it cool to about 40degC and, when required, add antibiotic near the flame.
• Store in the fridge and always check for contaminations before using.

LB medium (solid)

• For every 1 L of medium, add to a pyrex bottle or flask:
  • 10 g NaCl
  • 10 g bacto-tryptone
  • 5 g yeast extract
  • 15 g of agar
• Add 1 L of deionized water (blue tap of the Millipore machine).
• Autoclave (if using a bottle, do not close the cap completely).
• Let it cool to about 50degC and, when required, add antibiotic near the flame.
• Pour approximately 20 ml of medium in Petri dishes (try to avoid bubbles) and let them polymerize at room temperature.
• Cover with foil or parafilm, store in the fridge and always check for contaminations and overdrying before using.
• Use them within 1-2 months.