Magni:Protocols/sodiumacetate

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DNA purification (sodium acetate)

• Add 1/10 of the original volume of sodium acetate 3 M
• Add 2.5-fold of the original volume of absolute ethanol
• Freeze at -80degC for 30 min or at -20degC overnight
• Centrifuge at 13000 rpm, 4degC for 20 min
• Decant supernatant
• Add 250 ul of 70% ethanol
• Centrifuge at 13000 rpm, 4degC for 20 min
• Remove all supernatant with a pipette
• Air dry pellet until ethanol is totally removed
• Elute with 5-10 ul Milli-Q water