OpenWetWare/Page Updates/Raw Data/2005
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2005
04/2005
- Common: 165
- Groups: 0
- IGEM: 0
- Protocols: 72
- Materials: 24
- Classes: 10
- Labs: 27
Details
Group Pages
Lab Pages
- (26) Endy Lab
- (1) Knight Lab
IGEM Pages
Protocol Pages
- (35) Protocols
- (8) MIT:Sequencing BioBrick DNA
- (8) High Efficiency Transformation
- (4) Electrocompetent cells
- (4) RNA extraction
- (3) DNA ligation
- (2) SortoStat/Operation
- (2) Preparing chemically competent cells
- (1) Yeast Colony PCR
- (1) Yeast DNA Prep
- (1) Design a Microfluidic Chip
- (1) Agarose gel electrophoresis
- (1) Endy:Pouring plates
- (1) Agarose Pads for Microscopy
Class Pages
Material Pages
- (8) Materials
- (3) Lysozyme
- (2) Ethidium Bromide
- (2) Dyes and stains
- (1) Hoechst 33342
- (1) Acridine Orange
- (1) SYBR Green I
- (1) SYBR Green II
- (1) Carboxyfluorescein Diacetate
- (1) DAPI
- (1) Fluorescein diacetate
- (1) Propidium iodide
- (1) SYTO
Common Pages
- (41) Main Page
- (19) Drew Endy
- (16) Northern Blot
- (13) Strains
- (9) Library Construction Strain Box 1
- (8) Equipment
- (5) Jeffrey Gritton
- (5) Ilya Sytchev
- (4) Francois St-Pierre
- (4) Making scientific posters
- (4) Sriram Kosuri
- (3) Communicating Your Results
- (3) Categories
- (3) Vectors
- (2) Genotypes
- (2) Randy Rettberg
- (2) Barry Canton
- (2) Standard E. coli Strain for BioBricks
- (2) Sample Strain Box
- (2) Josh Michener
- (2) Jennifer Braff
- (2) Piped link
- (2) Probe Prep, 32P End-Labeled Propes
- (1) Nanodrop
- (1) Probe Prep, 32P End-Labeled Probes
- (1) Reporters
- (1) Tom Knight
- (1) Caitlin Conboy
- (1) Synchronized Oscillator Strain Box 1
- (1) Heather Keller
- (1) Samantha Sutton
- (1) TMT Thesis Project
- (1) RNA In Vitro Standards
05/2005
- Common: 147
- Groups: 0
- IGEM: 0
- Protocols: 107
- Materials: 30
- Classes: 106
- Labs: 38
Details
Group Pages
Lab Pages
- (36) Endy Lab
- (2) Knight Lab
IGEM Pages
Protocol Pages
- (15) Protocols
- (11) Order a Microfluidic Chip from the Foundry
- (10) SortoStat/Operation
- (8) Annealing primers
- (7) Designing primers
- (7) DNA ligation
- (6) Miniprep
- (6) Agarose gel electrophoresis
- (5) PNK Treatment of DNA Ends
- (5) Endy:Pouring plates
- (4) Purification of DNA
- (4) Preparing chemically competent cells
- (3) Design a Microfluidic Chip
- (3) Electroporation
- (3) Ethanol precipitation of small DNA fragments
- (2) Knight:Micropure EZ and Microcon purification
- (2) Cell Cycle Arrest
- (2) Endy:Templiphi
- (1) MIT:Sequencing BioBrick DNA
- (1) Phosphatase treatment of linearized vector
- (1) Electrocompetent cells
- (1) High Efficiency Transformation
Class Pages
Material Pages
- (10) Materials
- (3) Ampicillin
- (2) Rifampicin
- (2) TAE
- (2) Streptomycin
- (2) Kanamycin
- (2) Phleomycin
- (1) Antarctic Phosphatase
- (1) Dyes and stains
- (1) Ethidium Bromide
- (1) G418
- (1) TSS
- (1) Tetracycline
- (1) DNA ladder
Common Pages
- (26) Main Page
- (10) TABASCO
- (9) Summer 2005 Retreat
- (7) Rebuilding T7
- (6) How to publish a paper
- (5) Equipment
- (5) Measures and Models of T7 Gene Expression
- (5) Papers
- (4) Evolutionary Stability
- (4) LaTeX
- (4) Other Resources
- (3) Stimulator
- (3) Communicating Your Results
- (3) Current events
- (3) Visualization
- (3) PoPS
- (3) Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)
- (3) DSpace/Startup
- (3) Optimizing Sample for Sequencing
- (3) MIT CCR Biopolymers Laboratory
- (2) Arrest in G2/M phase using Nocodazole
- (2) Physiology of the Bacterial Cell
- (2) Microfluidic chemostat labview program
- (2) Software for papers, posters and talks
- (2) Genotypes
- (2) Time-Dependent Analysis of Signaling Pathways
- (2) Doing Research
- (2) MIT Flow Cytometer Core Facility
- (1) Latex
- (1) Agarose gel electrophoresis/Common issues
- (1) Useful Resources
- (1) Leon Chan
- (1) Loading dye
- (1) VectorNTI
- (1) Standard E.Coli Strain for BioBricks
- (1) How to Communicate
- (1) How to read a paper
- (1) How does T7 deal with Uncertainty
- (1) Research
- (1) A Short Course in Bacterial Genetics
- (1) RiPS
- (1) Categories
- (1) Communication
- (1) Talks
- (1) Genetic Stability
- (1) EcoRI/SpeI Double Digest
- (1) Molecular Cloning
06/2005
- Common: 548
- Groups: 16
- IGEM: 0
- Protocols: 102
- Materials: 59
- Classes: 1345
- Labs: 299
Details
Group Pages
Lab Pages
- (186) Endy Lab
- (56) Grossman Lab
- (24) Grossman Lab
- (16) Knight Lab
- (9) Knight
- (8) Endy Lab
IGEM Pages
Protocol Pages
- (18) Protocols
- (15) In vitro transcription with T7 RNA polymerase
- (12) DNA ligation
- (9) SortoStat/Operation
- (5) Studier Lysate Prep
- (5) Preparing chemically competent cells
- (5) MIT:Sequencing BioBrick DNA
- (4) Cesium Chloride Purification of T7
- (3) Questions and Answers
- (3) Agarose gel electrophoresis
- (3) Knight:DNA ligation using NEB Quick Ligation Kit
- (3) Knight:Annealing and primer extension with Klenow polymerase
- (2) Pulse-chase protein production
- (2) Colony PCR
- (2) Annealing primers
- (1) Knight:Micropure EZ and Microcon purification
- (1) Modeller
- (1) Knight:Colony PCR
- (1) Beta-galactosidase assay
- (1) Fixing cells
- (1) Electrocompetent cells
- (1) RNA extraction
- (1) Order a Microfluidic Chip from the Foundry
- (1) High Efficiency Transformation
- (1) Design a Microfluidic Chip
- (1) Phosphatase treatment of linearized vector
Class Pages
Material Pages
- (14) Materials
- (6) LB
- (5) Kanamycin
- (4) Raffinose
- (3) Neidhardt EZ Rich Defined
- (3) PBS
- (3) CFP
- (2) YFP
- (2) SC
- (2) ONPF
- (2) Erythromycin
- (2) MRFP1
- (1) M9 salts
- (1) IPTG
- (1) Dyes and stains
- (1) DNA ladder
- (1) SOC
- (1) T broth
- (1) TSS
- (1) Reverse Transcriptase
- (1) Phleomycin
- (1) YPD
- (1) SD
Common Pages
- (55) Main Page
- (32) EL2
- (30) Barcodes
- (19) Sortostat/Experiments/Early experiments
- (18) Computing/BioMicro Center
- (18) Summer 2005 Retreat
- (16) Main Page v2
- (14) Nikon TE2000 Microscope
- (14) Nanodrop
- (13) Strains
- (12) General Chemical Hygiene
- (12) Standard E. coli Strain for BioBricks
- (10) Building 68
- (9) Other Resources
- (8) DSpace/Startup
- (8) Current events
- (8) Biological Energy FAQ
- (7) Pronase
- (7) Lab Specific Chemical Hygiene
- (7) Classroom Training
- (7) Computing
- (6) Training Needs Assessment
- (5) Web Training
- (5) Innocent Bystanders
- (5) MIT Flow Cytometer Core Facility
- (4) Vectors
- (4) Department/building at MIT
- (4) JMB(340)707
- (4) Useful Cell-Cell Signaling Box
- (4) Equipment
- (4) Synchronized Oscillator Strain Box 1
- (4) How does T7 deal with Uncertainty
- (4) Chassis/System - BC Box 1
- (4) Note regarding Codon Devices, Inc.
- (4) Admin Team
- (4) New to MIT
- (3) Library Construction Strain Box 1
- (3) Marinade for Leon's Chicken Skewers
- (3) Pixel size calibration
- (3) Biology of Hydrogen Production
- (3) Sortostat/Image processing
- (3) MIT Certificate
- (3) Tutorials
- (3) Endy Lab Meeting 6/1/2005
- (3) General Biosafety for Researchers
- (3) Managing Hazardous Waste
- (3) Electrical Team
- (3) Precision Water Bath
- (3) Cell-Cell Signaling Box 1
- (3) Biology Team
- (2) Lab Specific Chemical Hygiene Training
- (2) Chemical Hygiene Plan
- (2) Safety Plan
- (2) Computing/Athena
- (2) Safety
- (2) Mess in GelRoom
- (2) Compliance Form
- (2) T7 Buffer
- (2) BioBrick Inventory
- (2) Jay Copeland
- (2) Parameter Estimation in Matlab
- (2) How to publish a paper
- (2) Probe Prep, 32P End-Labeled Probes
- (2) Will Bosworth
- (2) Gentamycin
- (2) Quantitative Microscopy
- (2) Marinade for Shish Kabob Beef and Veggie Skewers
- (2) Genotypes
- (2) Computing/Linux/Process Control
- (2) BEInG
- (2) Bioreactor Team
- (1) EtBr
- (1) Stuff I want to know about/things I need help with/protocols I need
- (1) Ryan Simkovsky
- (1) Sharps containers
- (1) Renewable energy
- (1) Ingrid Lawhorn
- (1) Randy Ewoldt
- (1) Peter Weigele
- (1) Software for papers, posters and talks
- (1) Jeff Gritton
- (1) Chicken Skewers
- (1) Endy Yeast
- (1) How to reduce agarose pad sliding
- (1) Polony
- (1) Announcements
- (1) Annealing complementary primers
- (1) May 2005
- (1) Marinade for Shrimp Skewers
- (1) Biohazardous waste
- (1) Making scientific posters
- (1) Chemical Hyiene Plan
- (1) Communicating Your Results
- (1) Lab Specific Training
- (1) Lab Chores
- (1) Larry Unsworth
- (1) Munhee Sohn
- (1) Tony Yu
- (1) Jim Serdy
- (1) Rosalind Takata
- (1) Michael Motion
- (1) Kurt Keville
- (1) Paolo Boccazzi
- (1) Bracken King
- (1) Andrea Tentner
- (1) Adam Lawrence
- (1) Philip Dextras
- (1) Nina Keshtvy
- (1) FITC/GFP Filter Set
- (1) CFP Filter Set
- (1) RFP Filter Set
- (1) Microfluidic chemostat labview program
- (1) Endy Lab Meeting 6/8/2005
- (1) YFP Filter Set
- (1) UV/DAPI Filter Set
- (1) Business and Policy Participation in Biological Energy
- (1) Engineering and Materials Science in Biological Energy
- (1) Goals of the BEInG
- (1) Focusing the Nikon TE2000 Condenser
- (1) Danielle France
- (1) Max Berniker
- (1) Group Meeting Snack Schedule
- (1) Lab Meetings
- (1) Safety References
- (1) Safety Announcements
- (1) Safety Training
- (1) BBQ Recipes
- (1) Summer 2005 Retreat.v2
- (1) Endy Lab Meeting 6/2/2005
- (1) Craig Venter's Synthetic Genomics plans to "program cells" to produce hydrogen
- (1) In the News
- (1) Talks
- (1) Lab Chores Listed by Lab Member
- (1) Lab Chores Listed by Task
- (1) Sotiris Koutsopoulos
- (1) Jared Toettcher
- (1) Shannon Flaugh
- (1) Rany Woo
- (1) Vinay Mahajan
- (1) Mats Cooper
- (1) Hydrogen Photobioreactor
- (1) Lab Supplies
- (1) Ligation
- (1) To Registry
- (1) PPT to PDF on a Mac
- (1) Radiation Training
07/2005
- Common: 359
- Groups: 46
- IGEM: 0
- Protocols: 82
- Materials: 14
- Classes: 1425
- Labs: 147
Details
Group Pages
- (18) BE.109
- (7) BEInG
- (5) Clarke
- (4) SfGS
- (4) Being
- (4) Luciferyeast
- (2) Tregwiki
- (1) WhoOrderedThat?
- (1) Light my wire
Lab Pages
- (130) Endy Lab
- (9) Endy Lab
- (3) Keating
- (3) Keating Lab
- (1) Grossman Lab
- (1) Knight Lab
IGEM Pages
Protocol Pages
- (15) Protocols
- (8) Site-directed mutagenesis
- (7) Bacterial cell culture
- (7) Colony PCR
- (6) Agarose gel electrophoresis
- (5) Preparing chemically competent cells
- (5) Restriction digest
- (5) Designing primers
- (4) DNA ligation
- (3) Phenol/chloroform extraction
- (3) Endy:STN Chemostat Protocol
- (3) Endy:Colony PCR
- (3) Endy:E. coli Western Blot
- (1) Miniprep
- (1) In vitro transcription with T7 RNA polymerase
- (1) Electrocompetent cells
- (1) Electroporation
- (1) Knight:Colony PCR
- (1) Yeast DNA Prep
- (1) Acrylamide gels
- (1) Knight:Restriction Digest
Class Pages
- (14) Students for Global Sustainability Wiki
- (11) The BioBricks Foundation
- (5) Synthetic Biology
- (3) Biological Energy Interest Group
- (2) SAVE Wiki
Material Pages
- (5) Phusion
- (3) Tetracycline
- (3) Materials
- (2) ATc
- (1) Arabinose
Common Pages
- (24) Synthetic Genomics Study
- (20) Retreat Transportation
- (16) July 17 picnic on Cape Cod
- (16) Genotypes
- (15) Error Detection & Correction in Replicating Machines
- (12) Bioreactor Team
- (12) Orthogonal cloning of clpXP
- (11) 2005 ICSB Conference
- (10) Main Page
- (9) E. coli restriction-modification system
- (9) SuperGroup
- (8) Registry Wish List
- (7) Past Events and planning information
- (6) DSpace/Startup
- (6) SfGS past events
- (6) Bioreactor Parameters
- (6) Device Details
- (6) Note regarding Codon Devices, Inc.
- (5) TABASCO
- (5) SAVE planning information
- (5) The SAVE Wiki
- (5) Tëst
- (4) Nanodrop
- (4) Luciferyeast
- (4) Strains
- (3) Intermittent circulation
- (3) Other Resources
- (3) Flakey DE Stuff
- (3) MIT Flow Cytometer Core Facility
- (3) Gas exchange
- (3) Shear stress
- (3) SAVE Affiliated Groups
- (3) SfGS upcoming events (and other ways to get involved)
- (3) Gas delivery to fuel cell
- (2) Who Ordered That?
- (2) Light My X,Y,&Z
- (2) Medium change
- (2) Cell density
- (2) Vectors
- (2) Membrane type
- (2) Power from fuel cell to pump
- (2) Cell size
- (2) Admin Team
- (2) Fuel cell type
- (2) Power consumption
- (2) Light levels
- (2) Valving, fittings, and tubing
- (2) Pumps
- (2) Strainer, plunger
- (2) Swimming
- (2) Media formula
- (2) Research
- (2) Standard E. coli Strain for BioBricks
- (2) Sriram Kosuri
- (2) Summer 2005 Retreat
- (2) Light My Wire
- (2) Barcodes
- (2) Chris Sequeira
- (2) Vessel type
- (2) SfGS planning information
- (1) Quantification of nucleic acids
- (1) Managing Hazardous Waste
- (1) Computing
- (1) Jessica Harpole
- (1) Gas chromatograph of headspace
- (1) Settling time
- (1) Enzyme selection for BioBricks digest
- (1) Computing/Linux/Gnome
- (1) Nikon TE2000 Microscope
- (1) Useful Cell-Cell Signaling Box
- (1) Categories
- (1) Rebecca Ward
- (1) SfGS publications
- (1) Current events
- (1) Bryan Urban
- (1) Amy Keating
- (1) Arrest in G2/M phase using Nocodazole
- (1) Electrocompetent Cells
- (1) SfGS active members
- (1) Yeast vectors
- (1) ClpXP cloning vectors
- (1) Strain cells
- (1) Sean Clarke
- (1) Steve Stoddard
- (1) How to Communicate
- (1) Equipment
- (1) Dan Bolon
- (1) PH requirements
- (1) Generation time
- (1) Annealing complementary primers
- (1) E. coli genotypes
- (1) Parameter Estimation in Matlab
- (1) SAVE past events
- (1) Previous Events
- (1) LaTeX
- (1) Philip Dextras
- (1) Senescence
- (1) Directions to Bird Bath
- (1) Temporary gas storage/bypass
- (1) Theoretical Power generation
- (1) Real time measurement of output/power
- (1) Gas purification
- (1) Gas diffuser
- (1) Sealing/gaskets
- (1) Temperature
- (1) Nicole Hori
- (1) Hydrogenases
- (1) Nitrogenases
- (1) Charge battery
08/2005
- Common: 565
- Groups: 53
- IGEM: 0
- Protocols: 79
- Materials: 7
- Classes: 584
- Labs: 514
Details
Group Pages
- (28) Light my XYZ
- (7) SfGS
- (6) Being
- (3) BEInG
- (3) BE.109
- (2) Atomic composition
- (1) Bbf
- (1) SbsB Atomic composition
- (1) SbpA Atomic composition
- (1) KM
Lab Pages
- (186) Endy Lab
- (181) Sauer Lab
- (50) Sauer Lab
- (42) Keating
- (26) Keating Lab
- (16) Grossman Lab
- (5) Knight Lab
- (3) Endy Lab
- (3) Grossman Lab
- (2) Knight
IGEM Pages
Protocol Pages
- (24) Methods to determine the size of an object in microns
- (13) Protocols
- (6) Sauer:Expression/purification of 35S-Met proteins
- (6) Endy:Victor3 plate reader
- (4) SortoStat/Operation
- (4) Preparing chemically competent cells
- (3) Sequencing DNA
- (3) Site-directed mutagenesis
- (2) RNA extraction
- (2) Endy:RNA extraction
- (2) Designing primers
- (2) Endy:Pouring plates
- (2) Agarose gel electrophoresis
- (1) Knight:Site-directed mutagenesis/Single site
- (1) Electrocompetent cells
- (1) Phosphatase treatment of linearized vector
- (1) Restriction digest
- (1) Endy:Colony PCR
- (1) Layered plates
Class Pages
- (15) The BioBricks Foundation
- (8) Students for Global Sustainability Wiki
- (6) Synthetic Biology
- (1) SAVE Wiki
- (1) Biological Energy Interest Group
Material Pages
- (2) Materials
- (1) Ethidium Bromide
- (1) Acridine Orange
- (1) DNTPs
- (1) G418
- (1) ProteinA Sepharose Beads
Common Pages
- (46) Bioinformatics of S-layer Proteins
- (30) BioBricks construction tutorial
- (23) Standardized GFP quantification
- (19) Error Detection & Correction in Replicating Machines
- (18) Main Page
- (18) B-Galactosidase Assay (A better Miller)
- (16) T7.1
- (14) T7.2
- (14) Equipment
- (11) SfGS lecture
- (11) Flakey DE Stuff
- (11) Sortostat
- (10) SfGS upcoming events (and other ways to get involved)
- (10) Coiled Coils that may dimeric or trimeric
- (9) Hey, cut it out
- (9) T7.1/Construction
- (9) Studio!Sus
- (8) T7.1/Specification
- (8) SfGS Midway Sign-up Sheet
- (8) Biostuff@mit.edu
- (8) SfGS August 26, 2005
- (7) OWW BioMicro Server Switch
- (7) Current events
- (7) Sortostat/Optimal sorting cutoffs
- (7) Coiled Coils from Paircoils
- (7) Other Parameters
- (7) β-Galactosidase Assay (A better Miller)
- (6) Who Ordered That?
- (6) Amino Acid Composition
- (6) Coiled Coils
- (6) Studiosus.pdf
- (6) RWE072605
- (5) 2005 ICSB Conference
- (5) Nikon TE2000 Microscope
- (5) Theoretical Power generation
- (5) Evolutionary Stability
- (5) Bioreactor Parameters
- (5) Rebuilding T7
- (4) Getting started on the microscope
- (4) July 17 picnic on Cape Cod
- (4) Synthetic Genomics Study
- (4) SfGS active members
- (4) Microfluidic chemostat labview program
- (4) Reserving a room at MIT
- (4) Streak plates
- (4) Agarose gel electrophoresis/Common issues
- (4) E. coli genotypes
- (3) COMPOSITIONAL ANALYSIS
- (3) Chassis/System - BC Box 1
- (3) Statistical Analysis of Protein Sequence
- (3) DSpace/Startup
- (3) Sortostat/Experiments/Early experiments
- (3) LaTeX
- (3) Device and system design to modulate evolutionary stability
- (3) DNA sequence design to modulate evolutionary stability
- (2) Extinction coefficients
- (2) Media formula
- (2) Nanodrop
- (2) Flourless chocolate cake
- (2) SfGS planning information
- (2) Endnote 8 to older version of Endnote files
- (2) Beta-Galactosidase Assay (A better Miller)
- (2) Valving, fittings, and tubing
- (2) KM's soapbox
- (2) Predicts the location of coiled-coil regions in amino acid sequences and classifies the predictions as dimeric or trimeric
- (2) Talks
- (2) SbsB Extinction coefficients
- (2) A Better SDS-PAGE
- (2) CHARGE DISTRIBUTIONAL ANALYSIS
- (2) Writing a letter of recommendation
- (2) SfGS minutes
- (2) SfGS publications
- (2) T7.1/Reannotation
- (2) Sortostat/Setting up a new chip
- (2) Isadora deese
- (2) Laser auto-alignment
- (1) Sean Moore
- (1) Ykhodor
- (1) Yevgenia Khodor
- (1) Enzymes
- (1) SbpA Amino Acid Composition
- (1) SbsB Other Parameters
- (1) SbpA Extinction coefficients
- (1) SbpA Other Parameters
- (1) Cell size
- (1) Kathleen McGinness
- (1) Chemicals
- (1) Bioreactor Team
- (1) Other Resources
- (1) TABASCO
- (1) Alan Grossman
- (1) Sri Kosuri
- (1) June 2005
- (1) Safety
- (1) Strain cells
- (1) Loading dye
- (1) July 2005
- (1) Light My X,Y,&Z
- (1) Solenoids
- (1) SbsB DNA Sequence
- (1) Julie Norville
- (1) Registry Wish List
- (1) Protein Sequence
- (1) SbpA Protein Sequence
- (1) SbpA DNA Sequence
- (1) Research
- (1) Site-directed Mutagenesis
- (1) Device Details
- (1) Pixel size calibration
- (1) Electroporator
- (1) 5810R Centrifuge
- (1) Designing Primers
- (1) Enzyme selection for BioBrick digest
- (1) Restriction Digest
- (1) Absorbance Labels
- (1) Tutorials
- (1) SbsB Protein Sequence
- (1) Sap Protein Sequence
- (1) Flow cell
- (1) Alex Mallet
- (1) Ania Labno
- (1) Strains
- (1) SfGS past events
- (1) E. coli Genotypes
- (1) Genotypes
- (1) REPETITIVE STRUCTURES, MULTIPLETS, PERIODICITY ANALYSIS, SPACING ANALYSIS
- (1) SbsB Amino Acid Composition
09/2005
- Common: 616
- Groups: 217
- IGEM: 0
- Protocols: 57
- Materials: 1
- Classes: 673
- Labs: 763
Details
Group Pages
- (143) Eigencluster
- (26) Suggs
- (15) Matsudaira
- (9) Burge
- (6) Ellington
- (6) SfGS
- (3) Tregwiki
- (3) KM
- (3) SB
- (2) Bbf
- (1) BE.109
Lab Pages
- (305) Endy Lab
- (200) Silver Lab
- (70) Sauer Lab
- (54) Silver Lab
- (27) Sauer Lab
- (18) Lauffenburger Lab
- (17) Suggs Lab
- (12) Keating
- (11) Lauffenburger Lab
- (10) Matsudaira Lab
- (10) Ellington Lab
- (8) Knight Lab
- (4) Endy Lab
- (4) Burge Lab
- (3) Emelianov Lab
- (3) Rao Lab
- (3) Knight
- (1) Grossman Lab
- (1) Keasling Lab
- (1) Hu Lab
- (1) Grossman Lab
IGEM Pages
Protocol Pages
- (8) Protocols
- (6) Knight:TOPO TA cloning
- (4) Ethanol precipitation of nucleic acids
- (4) Site-directed mutagenesis
- (3) Silver: Lysate for Western
- (3) Acrylamide gels
- (3) Knight:Agarose gel electrophoresis
- (2) Sauer:Expression/purification of 35S-Met proteins
- (2) DNA ligation
- (2) PCR
- (2) Yeast Colony PCR
- (2) Colony PCR
- (2) Agarose gel electrophoresis
- (2) PNK Treatment of DNA Ends
- (1) Silver: Time-Lapse Microscopy
- (1) Silver: Colony PCR
- (1) Silver: Coomassie Stain
- (1) Silver: Destain
- (1) Agarose Pads for Microscopy
- (1) Sequencing DNA
- (1) MIT:Sequencing BioBrick DNA
- (1) Studier Lysate Prep
- (1) Endy:Victor3 plate reader
- (1) Knight:Annealing and primer extension with Klenow polymerase
- (1) Restriction digest
- (1) Silver: PCR
Class Pages
- (84) Synthetic Biology
- (11) Students for Global Sustainability Wiki
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- (3) SAVE Wiki
Material Pages
- (1) T broth
Common Pages
- (62) Main Page
- (38) The SAVE Wiki
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- (31) Yeast rebuild
- (29) AlexLabNotebook/ChrIIIRebuild
- (29) MIT Biological Engineering Graduate Student Board
- (28) Logo Ideas
- (26) Main Page v2
- (21) 'Round-the-horn site-directed mutagenesis
- (16) Jenny Nguyen
- (16) AlexLabNotebook/ChrIIIRebuild/9/24/05-9/30/05
- (13) Organic Iron Chef
- (12) Doug Lauffenburger
- (12) Earth Day at MIT
- (10) Sriram Kosuri
- (9) Current events
- (8) Clemens Foerst
- (8) Pam Silver
- (6) Rebuilding T7
- (6) Computing/BioMicro Center
- (6) AlexLabNotebook/ChrIIIRebuild/9/19/05-9/23/05
- (5) Erico Santos
- (5) T7.2
- (5) Error Detection & Correction in Replicating Machines
- (4) 2. Who does this affect?
- (4) SfGS lecture
- (4) Erico Santos.doc
- (4) Simple wiki editing examples
- (3) Crews Lab
- (3) E. coli genotypes
- (3) Flakey DE Stuff
- (3) Jeff Tabor
- (3) Chassis/System - BC Box 1
- (3) Synthetic Genomics Study
- (3) SfGS minutes
- (3) Biostuff@mit.edu
- (3) KM's soapbox
- (3) The Auditors are coming to MIT - Audit FAQ
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- (3) Ovidiu Bujorean
- (3) Noam Shomron
- (2) PTrc99A
- (2) Kathleen McGinness
- (2) SAVE active members
- (2) 1. What is an external (Level III) audit?
- (2) SfGS upcoming events (and other ways to get involved)
- (2) VectorNTI
- (2) EHS External Audit update
- (2) Laser auto-alignment
- (2) David Lucchino
- (2) Ideas for wiki archive
- (2) Maxine Jonas
- (2) ECM Analogs
- (2) Optical Trapping
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- (2) Lab Meetings
- (2) Amy Nichols
- (1) Eric Alm
- (1) Cell Motion
- (1) Quantomix
- (1) PET24d
- (1) Mikhail Shapiro
- (1) Hyung-Do Kim
- (1) May 2005
- (1) PBAD24
- (1) PET21b
- (1) Nigela Atkinson
- (1) PET14b
- (1) Supergroup Mon Sep 26
- (1) Computing/Athena
- (1) Liquid Media
- (1) Sampsa Hautaniemi
- (1) Rickard Sandberg
- (1) Pamela Silver
- (1) June 2005
- (1) BioBricks construction tutorial
- (1) Diana Chai
- (1) Michael Stadler
- (1) Zefeng Wang
- (1) Grace Zheng
- (1) Vivian Tung
- (1) Chris Burge
- (1) Ben Lewis
- (1) Jianming Zhang
- (1) SAVE useful documents
- (1) Grace Xiao
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- (1) 14. I am an office worker, how does the audit affect me?
- (1) Chris Rao
- (1) Brad Friedman
- (1) Nate Tedford
- (1) BBF
- (1) OWW BioMicro Server Switch
- (1) Cydney Nielsen
- (1) Refactoring T7
- (1) Judy Yeh
- (1) Registry of Standard Biological Models
- (1) Tutorials
- (1) T7.1
- (1) Sophia Roosth
- (1) Hydrogen Photobioreactor
- (1) Nikon TE2000 Microscope
- (1) Shan Wu
- (1) David Appleyard
- (1) Megan McBee
- (1) Nancy Guillen
- (1) Jordan Green
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- (1) Lab meetings
- (1) Research
- (1) Ira Phillips
- (1) Megan Palmer
- (1) Annealing complementary primers
- (1) The Team
- (1) 16. How can I find out who my EHS Representative is?
- (1) 17. What is the EHS Policy and where can I find it?
- (1) 15. Where can I find my EHS Coordinator’s contact information?
- (1) 13. What if I am a UROP?
- (1) 12. What happens if I am in the middle of an experiment and the auditors want to speak with me? (Or will this interrupt my lab?)
- (1) RWE083005
- (1) Papers
- (1) Communicating science to the public
- (1) August 2005
- (1) Communicating Your Results
- (1) Old plates in cold room
- (1) How to run a group meeting
- (1) 11. Will I get advance notice?
- (1) 10. What happens if I don’t give the right answer or known the answer to a question?
- (1) Competitors
- (1) 3. Where will the auditors be inspecting?
- (1) Market Analysis and Strategy
- (1) Technology
- (1) Project Description
- (1) 4. Who performs the external (Level III) audit?
- (1) 5. How does this affect me?
- (1) 9. Will they talk to my PI/Supervisor?
- (1) 8. What types of questions will be asked
- (1) 7. What should I do if the auditors ask me questions?
- (1) 6. What can I do to prepare?
- (1) July 2005
10/2005
- Common: 339
- Groups: 135
- IGEM: 0
- Protocols: 40
- Materials: 0
- Classes: 268
- Labs: 228
Details
Group Pages
- (45) Eigencluster
- (37) Suggs
- (18) Matsudaira
- (11) Keasling
- (9) Other Stuff Re
- (7) BE.109
- (3) Rao
- (2) MIT
- (1) SfGS
- (1) Wikiomics
- (1) Tregwiki
Lab Pages
- (110) Endy Lab
- (41) Knight Lab
- (31) Silver Lab
- (24) Sauer Lab
- (11) Keating
- (4) Keasling Lab
- (2) Matsudaira Lab
- (2) Knight
- (1) Rao Lab
- (1) Grossman Lab
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IGEM Pages
Protocol Pages
- (25) Library Generation
- (2) Endy:STN Chemostat Protocol
- (2) Protocols
- (2) Miniprep
- (2) Endy:Victor3 plate reader
- (1) Endy:Pouring plates
- (1) Pulse-chase protein production
- (1) Knight:TOPO TA cloning
- (1) Preparing chemically competent cells
- (1) Endy:RNA extraction
- (1) SortoStat/Operation
- (1) Questions and Answers
Class Pages
Material Pages
Common Pages
- (40) T7.2/gene specification
- (32) Publishing Group
- (30) Parts characterization
- (28) BE.180
- (13) AlexLabNotebook/ChrIIIRebuild/10/1/05-10/7/05
- (11) Titratable control of pBAD and lac promoters in individual E. coli cells
- (10) Bacterial artificial chromosomes
- (9) AlexLabNotebook/ChrIIIRebuild/9/24/05-9/30/05
- (9) AlexLabNotebook/ChrIIIRebuild
- (9) Main Page
- (9) Pam Silver
- (7) AlexLabNotebook/Pheromone
- (6) Lauren Ha
- (6) AlexLabNotebook/YeastRebuild
- (6) MIT Biological Engineering Graduate Student Board
- (5) CSBI.100
- (5) Publishing Group/Meeting 10-6-05
- (5) TMT Thesis Project
- (4) Codon Optimized for Failure
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- (4) T7.2
- (4) Parts characterization/Characterization approaches
- (4) Useful facts
- (4) Nikon TE2000 Microscope
- (4) Equipment
- (3) Jenny Nguyen
- (3) Beta-Galactosidase Assay (A better Miller)
- (3) Luis Alvarez
- (3) Ved Sharma
- (3) More
- (3) AlexLabNotebook/ChrIIIRebuild/9/19/05-9/23/05
- (3) SfGS lecture
- (3) Current events
- (3) Parts characterization/Measurement techniques
- (3) Publishing Group/Thoughts
- (3) Publishing Group/Meeting 10-4-05
- (3) Chassis/System - BC Box 1
- (2) Pour YPD plates
- (2) Lab meetings
- (2) Synthetic Genomics Study
- (1) T7 Uncertainty
- (1) AlexLabNotebook/YeastRebuild/9/19/05-9/23/05
- (1) AlexLabNotebook/YeastRebuild/9/24/05-9/30/05
- (1) AlexLabNotebook/YeastRebuild/10/1/05-10/7/05
- (1) Denaturing acrylamide gel purification of nucleic acids
- (1) Linder Lab
- (1) Copyright
- (1) Rune Linding
- (1) Googlesearchheader
- (1) DNA sequence design to modulate evolutionary stability
- (1) Andrew Hessel
- (1) Tabasco
- (1) AlexLabNotebook/9/24/05-9/30/05
- (1) Vectors
- (1) CcdB
- (1) Supergroup Mon Oct 17
- (1) Counter-selectable markers
- (1) September 2005
- (1) Parts Characterization
- (1) Computing
- (1) Registry Wish List
- (1) 2005 ICSB Conference
- (1) AlexLabNotebook/9/19/05-9/23/05
- (1) Visualization
- (1) AlexLabNotebook
- (1) Barry Canton
- (1) Sriram Kosuri
- (1) How to publish a paper
- (1) AlexLabNotebook/10/1/05-10/7/05
11/2005
- Common: 701
- Groups: 147
- IGEM: 0
- Protocols: 61
- Materials: 14
- Classes: 185
- Labs: 294
Details
Group Pages
- (88) BE.109
- (25) Matsudaira
- (12) Bell
- (8) Suggs
- (4) SfGS
- (3) Bcanton
- (2) Tregwiki
- (2) 20.109(S07)
- (2) Ellington
- (1) Wikiomics
Lab Pages
- (156) Endy Lab
- (59) Sauer Lab
- (36) Silver Lab
- (18) Silver Lab
- (13) Knight Lab
- (3) Van Oudenaarden Lab
- (2) Van Oudenaarden Lab
- (2) Keating
- (1) Schmidt Lab
- (1) Knight
- (1) Matsudaira Lab
- (1) Knight Lab
- (1) Keating Lab
IGEM Pages
Protocol Pages
- (20) Endy:FACS calibration with beads
- (9) Protocols
- (6) Site-directed mutagenesis
- (5) Methods to determine the size of an object in microns
- (3) Phenol/chloroform extraction
- (3) Ethanol precipitation of nucleic acids
- (2) Knight:Micropure EZ and Microcon purification
- (2) Knight:Colony PCR
- (2) Endy:Victor3 plate reader
- (2) Preparing chemically competent cells
- (2) In vitro transcription with T7 RNA polymerase
- (1) Assaying mating
- (1) High Efficiency Transformation
- (1) Order a Microfluidic Chip from the Foundry
- (1) Resuspension of primers
- (1) Endy:Pouring plates
Class Pages
- (25) Synthetic Biology
- (7) SynBERC
- (4) BE Board
- (4) Bioinformatics Discussion Group
- (2) IGEM
- (1) Students for Global Sustainability Wiki
Material Pages
- (5) Ampicillin
- (4) TE
- (4) Materials
- (1) Neidhardt EZ Rich Defined
Common Pages
- (216) ICampus Application for OWW
- (69) Science 2.0/Brainstorming
- (42) Adventure Background
- (35) Science 2.0
- (30) Searching the literature
- (23) Ron Weiss Visit
- (21) Natalie Kuldell
- (21) Main Page
- (16) Adventures
- (14) Jeff Tabor
- (13) BE.109
- (10) Jenny Nguyen
- (9) Sortostat
- (9) Flakey DE Stuff
- (9) MEG
- (8) Science 2.0/Web 2.0 analogy
- (8) Bacterial artificial chromosomes
- (8) Bjorn Millard
- (7) Current events
- (7) Carl Schmidt
- (7) Vectors
- (6) SfGS ERC White Paper
- (6) Pour YPD plates
- (5) Converting documents to mediawiki markup
- (5) Jeff Tabor/Pics
- (5) Registry Wish List
- (5) BSO
- (4) Hydrogen Photobioreactor
- (4) E. coli genotypes
- (4) Synthetic Genomics Study
- (4) MIT Biological Engineering Graduate Student Board
- (3) Stimulator
- (3) Computing/Athena
- (3) ERC White Paper
- (3) David Lowry
- (3) Sriram Kosuri
- (3) BE.180
- (3) ICampus Application for OWW/Official wiki version
- (2) Overlap Extension PCR
- (2) Nandita Mullapudi
- (2) Preparing for Level II inspections
- (2) Michael O'Kelly
- (2) Sortostat/Experiments/Early experiments
- (2) SfGS planning information
- (2) Computing/BioMicro Center
- (2) TMT Thesis Project
- (2) Screening Plasmids
- (2) Equipment
- (2) Research
- (1) Ian Molineux
- (1) Industrial Seminar Series with Mark Trusheim
- (1) John Tsang
- (1) Bioinformatics
- (1) Eric Spear
- (1) Chris Luttiig
- (1) Codon Optimized for Failure
- (1) Error Detection & Correction in Replicating Machines
- (1) ICampus Application for OWW/Official plain text version
- (1) Publishing Group/Thoughts
- (1) Sabrina Spencer
- (1) How does T7 deal with Uncertainty
- (1) SortoStat experiments
- (1) Setting up a new sortostat chip
- (1) Sortostat/Image processing
- (1) Sortostat Image Processing
- (1) Operate Microfluidic Chemostat
- (1) Chassis/System - BC Box 1
- (1) Synthetic Society
- (1) Strains
- (1) Kathleen McGinness
- (1) Supergroup Tue Nov 22
- (1) Publishing Group
- (1) Papers
- (1) Useful facts
- (1) Nanodrop
- (1) Science 2.0/vSri
- (1) Brenda Pepe
12/2005
- Common: 931
- Groups: 371
- IGEM: 0
- Protocols: 30
- Materials: 19
- Classes: 347
- Labs: 509
Details
Group Pages
- (88) BE.109
- (71) Sosnick
- (53) Chandra
- (53) University of Chicago
- (23) Labs
- (19) Pan
- (10) DeLong
- (8) Bbf
- (8) Eduardo A Silva
- (6) Eigencluster
- (5) Suma
- (5) Dewikify
- (5) MIT
- (3) Keasling
- (2) Bcanton
- (2) Matsudaira
- (2) Tregwiki
- (2) Eduardo A. Silva
- (2) Rao
- (2) KM
- (1) Fhuang
- (1) Wikiomics
Lab Pages
- (148) Lauffenburger Lab
- (98) Endy Lab
- (98) Silver Lab
- (37) Sosnick Lab
- (29) Keating
- (17) Sauer Lab
- (16) Pam Kreeger
- (8) Silver Lab
- (7) Chandra Lab
- (7) Knight Lab
- (7) DeLong Lab
- (5) Rao Lab
- (3) Van Oudenaarden Lab
- (3) Grossman Lab
- (2) Pan Lab
- (2) Keasling Lab
- (2) Sauer Lab
- (2) Keating Lab
- (2) Matsudaira Lab
- (2) Endy Lab
- (2) Endy
- (2) Lauffenburger Lab
- (2) Francis Lab
- (2) Knight
- (1) Bradley Lab
- (1) Silver
- (1) Lauffenburger
- (1) Suggs Lab
- (1) Sauer
- (1) Grossman
IGEM Pages
Protocol Pages
- (9) Protocols
- (7) MIT:Sequencing BioBrick DNA
- (4) Keating:Experimental Protocols:HPLC
- (3) Endy:Victor3 plate reader
- (3) Sequencing DNA
- (1) Dropout plates for yeast
- (1) Electrocompetent cells
- (1) PNK Treatment of DNA Ends
- (1) Knight:Preparing electrocompetent cells
Class Pages
- (79) Mimulus Community
- (48) The BioBricks Foundation
- (10) BE Board
- (4) Synthetic Biology
- (2) Bioinformatics Discussion Group
- (1) SAVE Wiki
Material Pages
- (4) Materials
- (3) Tetracycline
- (3) Kanamycin
- (3) Ampicillin
- (1) Rifampicin
- (1) Silver: 50x TAE
- (1) Mesoplama florum:Culture media
- (1) Nalidixic Acid
- (1) Trimethoprim
- (1) Chloramphenicol
Common Pages
- (182) E. coli genotypes
- (73) David Lowry
- (43) Main Page
- (40) LightCannon
- (27) Jeff Tabor
- (22) Main Page v2
- (21) Jean-Claude Bradley
- (20) University of Chicago
- (19) Identifiers
- (19) Datasheets
- (17) T7.1/Evolution
- (16) MIT Underwater
- (16) Karthik Raman
- (13) Sriram Kosuri
- (12) Poly A RNA in situ protocol
- (11) Kalidas Y
- (10) LisaJoslin
- (10) SfGS ERC White Paper
- (10) Career resources
- (9) MIT
- (9) Flakey DE Stuff
- (8) Hyung-Do Kim
- (8) BjornsMethods/ExperimentLog
- (7) Beta-Galactosidase Assay (A better Miller)
- (7) Registry Wish List
- (6) Doug Lauffenburger
- (6) Bjorn Millard
- (6) OpenWetWare
- (6) Web resources
- (6) Devin Strickland
- (5) Natalie Kuldell
- (5) Melissa Kemp
- (5) Nate Tedford
- (5) Searching the literature
- (5) Michael Baxa
- (5) Franklin Huang
- (4) MCB100
- (4) Christina Lewis
- (4) UsefulChem
- (4) BE.109
- (4) Kevin Wright
- (4) Jeff Tabor/Pics
- (4) DSpace
- (4) Ben Cosgrove
- (4) Karen Sachs
- (4) Kathryn Miller
- (4) Ben Capraro
- (4) KM's soapbox
- (4) Lucia Wille
- (4) BBF
- (3) Synthetic Society
- (3) JMB(340)707
- (3) Maria Foley
- (3) SAVE upcoming events (and other ways to get involved)
- (3) University of California, Berkeley
- (3) Harvard
- (3) Ed Delong
- (3) Catherine Cresson
- (3) ICampus Application for OWW
- (3) Sampsa Hautaniemi
- (3) John Burke
- (3) Alejandro Wolf-Yadlin
- (3) Ericka Noonan
- (3) Robin Prince
- (3) Matt Lazzara
- (3) Kirsty Smith
- (3) Bree Aldridge
- (3) Neil Kumar
- (3) Kevin Janes
- (3) Chris Rao
- (3) Artemis Kalezi
- (3) Muhammad H. Zaman
- (2) D. Vinh Pham
- (2) Tsultrim Palden
- (2) Vectors
- (2) Dennis Ryan
- (2) T7.1/PhageTitering
- (2) Preethi Rajagopalan
- (2) Nathan ST
- (2) Stuart Sealfon
- (2) BjornsMethods/PhageStorage
- (2) BjornsMethods/LargeBacterialCulture
- (2) Yeast rebuild
- (2) Lisa Joslin
- (2) Tracy Mincer
- (2) Kathleen McGinness
- (2) Jen Braff
- (2) Fei Hua
- (2) Jay McCarren
- (2) Computing
- (2) BioBricks construction tutorial
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- (2) University of Texas, Austin
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- (2) University of Illinois, Urbana-Champaign
- (2) Adventure Background
- (2) Suma
- (2) Steven Hallam
- (2) Jennifer Braff
- (2) Kostas Konstantinidis
- (2) SheungYan S. Lee
- (2) Asuncion Martinez
- (1) Grossman1995
- (1) Virginia Rich
- (1) Sumanta Mukherjee
- (1) Grossman1990
- (1) LaTeX
- (1) Eduardo A. Silva
- (1) Grossman2000
- (1) Grossman2005
- (1) Michael O'Kelly
- (1) Easilva
- (1) Suma Lab
- (1) Rinaldo Pereira
- (1) Cell Cycle Regulation
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- (1) John Zaborske
- (1) Biohacking
- (1) Yanmei Shi
- (1) Jeremy Seto
- (1) Peter D'Eustachio
- (1) Matsudaira
- (1) Prashant Khodade
- (1) Ed DeLong
- (1) Nagasuma Chandra
- (1) Cell Cell Signaling
- (1) Grossman Alumni
- (1) Grossman Current Members
- (1) Nirmal Kumar S
- (1) Bbf
- (1) Network Layer Model
- (1) Randy Rettberg
- (1) Adventures
- (1) Equipment
- (1) BioBricks Foundation Frequently Asked Questions
- (1) MIT/Equipment
- (1) Dewikify
- (1) News
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- (1) Genotypes
- (1) Getting Started on OpenWetWare
- (1) OpenWetWare Meeting Notes
- (1) Why join OpenWetWare?
- (1) Ideas for Wiki
- (1) Barry Canton
- (1) MIT Biological Engineering Graduate Student Board
- (1) T7.1
- (1) Sosnick
- (1) DSpace community
- (1) BjornsMethods/PhageTitering
- (1) Error Detection & Correction in Replicating Machines
- (1) BjornsMethods/SmallOvernight
- (1) Pam Silver
- (1) Chandra
- (1) Rao
- (1) BioBricks abstraction hierarchy
- (1) Paras Chopra
- (1) Aran Parillo
- (1) Pan
- (1) BjornsMethods/LargeOvernight