OpenWetWare/Page Updates/Raw Data/2005

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2005

04/2005

Common: 165
Groups: 0
IGEM: 0
Protocols: 72
Materials: 24
Classes: 10
Labs: 27

Details

Group Pages

Lab Pages

(26) Endy Lab
(1) Knight Lab

IGEM Pages

Protocol Pages

(35) Protocols
(8) MIT:Sequencing BioBrick DNA
(8) High Efficiency Transformation
(4) Electrocompetent cells
(4) RNA extraction
(3) DNA ligation
(2) SortoStat/Operation
(2) Preparing chemically competent cells
(1) Yeast Colony PCR
(1) Yeast DNA Prep
(1) Design a Microfluidic Chip
(1) Agarose gel electrophoresis
(1) Endy:Pouring plates
(1) Agarose Pads for Microscopy

Class Pages

Material Pages

Common Pages

05/2005

Common: 147
Groups: 0
IGEM: 0
Protocols: 107
Materials: 30
Classes: 106
Labs: 38

Details

Group Pages

Lab Pages

(36) Endy Lab
(2) Knight Lab

IGEM Pages

Protocol Pages

(15) Protocols
(11) Order a Microfluidic Chip from the Foundry
(10) SortoStat/Operation
(8) Annealing primers
(7) Designing primers
(7) DNA ligation
(6) Miniprep
(6) Agarose gel electrophoresis
(5) PNK Treatment of DNA Ends
(5) Endy:Pouring plates
(4) Purification of DNA
(4) Preparing chemically competent cells
(3) Design a Microfluidic Chip
(3) Electroporation
(3) Ethanol precipitation of small DNA fragments
(2) Knight:Micropure EZ and Microcon purification
(2) Cell Cycle Arrest
(2) Endy:Templiphi
(1) MIT:Sequencing BioBrick DNA
(1) Phosphatase treatment of linearized vector
(1) Electrocompetent cells
(1) High Efficiency Transformation

Class Pages

Material Pages

Common Pages

06/2005

Common: 548
Groups: 16
IGEM: 0
Protocols: 102
Materials: 59
Classes: 1345
Labs: 299

Details

Group Pages

(10) BEInG
(5) Bbf
(1) Seminar

Lab Pages

(186) Endy Lab
(56) Grossman Lab
(24) Grossman Lab
(16) Knight Lab
(9) Knight
(8) Endy Lab

IGEM Pages

Protocol Pages

(18) Protocols
(15) In vitro transcription with T7 RNA polymerase
(12) DNA ligation
(9) SortoStat/Operation
(5) Studier Lysate Prep
(5) Preparing chemically competent cells
(5) MIT:Sequencing BioBrick DNA
(4) Cesium Chloride Purification of T7
(3) Questions and Answers
(3) Agarose gel electrophoresis
(3) Knight:DNA ligation using NEB Quick Ligation Kit
(3) Knight:Annealing and primer extension with Klenow polymerase
(2) Pulse-chase protein production
(2) Colony PCR
(2) Annealing primers
(1) Knight:Micropure EZ and Microcon purification
(1) Modeller
(1) Knight:Colony PCR
(1) Beta-galactosidase assay
(1) Fixing cells
(1) Electrocompetent cells
(1) RNA extraction
(1) Order a Microfluidic Chip from the Foundry
(1) High Efficiency Transformation
(1) Design a Microfluidic Chip
(1) Phosphatase treatment of linearized vector

Class Pages

Material Pages

(14) Materials
(6) LB
(5) Kanamycin
(4) Raffinose
(3) Neidhardt EZ Rich Defined
(3) PBS
(3) CFP
(2) YFP
(2) SC
(2) ONPF
(2) Erythromycin
(2) MRFP1
(1) M9 salts
(1) IPTG
(1) Dyes and stains
(1) DNA ladder
(1) SOC
(1) T broth
(1) TSS
(1) Reverse Transcriptase
(1) Phleomycin
(1) YPD
(1) SD

Common Pages

07/2005

Common: 359
Groups: 46
IGEM: 0
Protocols: 82
Materials: 14
Classes: 1425
Labs: 147

Details

Group Pages

(18) BE.109
(7) BEInG
(5) Clarke
(4) SfGS
(4) Being
(4) Luciferyeast
(2) Tregwiki
(1) WhoOrderedThat?
(1) Light my wire

Lab Pages

IGEM Pages

Protocol Pages

(15) Protocols
(8) Site-directed mutagenesis
(7) Bacterial cell culture
(7) Colony PCR
(6) Agarose gel electrophoresis
(5) Preparing chemically competent cells
(5) Restriction digest
(5) Designing primers
(4) DNA ligation
(3) Phenol/chloroform extraction
(3) Endy:STN Chemostat Protocol
(3) Endy:Colony PCR
(3) Endy:E. coli Western Blot
(1) Miniprep
(1) In vitro transcription with T7 RNA polymerase
(1) Electrocompetent cells
(1) Electroporation
(1) Knight:Colony PCR
(1) Yeast DNA Prep
(1) Acrylamide gels
(1) Knight:Restriction Digest

Class Pages

(14) Students for Global Sustainability Wiki
(11) The BioBricks Foundation
(5) Synthetic Biology
(3) Biological Energy Interest Group
(2) SAVE Wiki

Material Pages

(5) Phusion
(3) Tetracycline
(3) Materials
(2) ATc
(1) Arabinose

Common Pages

08/2005

Common: 565
Groups: 53
IGEM: 0
Protocols: 79
Materials: 7
Classes: 584
Labs: 514

Details

Group Pages

Lab Pages

(186) Endy Lab
(181) Sauer Lab
(50) Sauer Lab
(42) Keating
(26) Keating Lab
(16) Grossman Lab
(5) Knight Lab
(3) Endy Lab
(3) Grossman Lab
(2) Knight

IGEM Pages

Protocol Pages

(24) Methods to determine the size of an object in microns
(13) Protocols
(6) Sauer:Expression/purification of 35S-Met proteins
(6) Endy:Victor3 plate reader
(4) SortoStat/Operation
(4) Preparing chemically competent cells
(3) Sequencing DNA
(3) Site-directed mutagenesis
(2) RNA extraction
(2) Endy:RNA extraction
(2) Designing primers
(2) Endy:Pouring plates
(2) Agarose gel electrophoresis
(1) Knight:Site-directed mutagenesis/Single site
(1) Electrocompetent cells
(1) Phosphatase treatment of linearized vector
(1) Restriction digest
(1) Endy:Colony PCR
(1) Layered plates

Class Pages

(15) The BioBricks Foundation
(8) Students for Global Sustainability Wiki
(6) Synthetic Biology
(1) SAVE Wiki
(1) Biological Energy Interest Group

Material Pages

Common Pages

(46) Bioinformatics of S-layer Proteins
(30) BioBricks construction tutorial
(23) Standardized GFP quantification
(19) Error Detection & Correction in Replicating Machines
(18) Main Page
(18) B-Galactosidase Assay (A better Miller)
(16) T7.1
(14) T7.2
(14) Equipment
(11) SfGS lecture
(11) Flakey DE Stuff
(11) Sortostat
(10) SfGS upcoming events (and other ways to get involved)
(10) Coiled Coils that may dimeric or trimeric
(9) Hey, cut it out
(9) T7.1/Construction
(9) Studio!Sus
(8) T7.1/Specification
(8) SfGS Midway Sign-up Sheet
(8) Biostuff@mit.edu
(8) SfGS August 26, 2005
(7) OWW BioMicro Server Switch
(7) Current events
(7) Sortostat/Optimal sorting cutoffs
(7) Coiled Coils from Paircoils
(7) Other Parameters
(7) β-Galactosidase Assay (A better Miller)
(6) Who Ordered That?
(6) Amino Acid Composition
(6) Coiled Coils
(6) Studiosus.pdf
(6) RWE072605
(5) 2005 ICSB Conference
(5) Nikon TE2000 Microscope
(5) Theoretical Power generation
(5) Evolutionary Stability
(5) Bioreactor Parameters
(5) Rebuilding T7
(4) Getting started on the microscope
(4) July 17 picnic on Cape Cod
(4) Synthetic Genomics Study
(4) SfGS active members
(4) Microfluidic chemostat labview program
(4) Reserving a room at MIT
(4) Streak plates
(4) Agarose gel electrophoresis/Common issues
(4) E. coli genotypes
(3) COMPOSITIONAL ANALYSIS
(3) Chassis/System - BC Box 1
(3) Statistical Analysis of Protein Sequence
(3) DSpace/Startup
(3) Sortostat/Experiments/Early experiments
(3) LaTeX
(3) Device and system design to modulate evolutionary stability
(3) DNA sequence design to modulate evolutionary stability
(2) Extinction coefficients
(2) Media formula
(2) Nanodrop
(2) Flourless chocolate cake
(2) SfGS planning information
(2) Endnote 8 to older version of Endnote files
(2) Beta-Galactosidase Assay (A better Miller)
(2) Valving, fittings, and tubing
(2) KM's soapbox
(2) Predicts the location of coiled-coil regions in amino acid sequences and classifies the predictions as dimeric or trimeric
(2) Talks
(2) SbsB Extinction coefficients
(2) A Better SDS-PAGE
(2) CHARGE DISTRIBUTIONAL ANALYSIS
(2) Writing a letter of recommendation
(2) SfGS minutes
(2) SfGS publications
(2) T7.1/Reannotation
(2) Sortostat/Setting up a new chip
(2) Isadora deese
(2) Laser auto-alignment
(1) Sean Moore
(1) Ykhodor
(1) Yevgenia Khodor
(1) Enzymes
(1) SbpA Amino Acid Composition
(1) SbsB Other Parameters
(1) SbpA Extinction coefficients
(1) SbpA Other Parameters
(1) Cell size
(1) Kathleen McGinness
(1) Chemicals
(1) Bioreactor Team
(1) Other Resources
(1) TABASCO
(1) Alan Grossman
(1) Sri Kosuri
(1) June 2005
(1) Safety
(1) Strain cells
(1) Loading dye
(1) July 2005
(1) Light My X,Y,&Z
(1) Solenoids
(1) SbsB DNA Sequence
(1) Julie Norville
(1) Registry Wish List
(1) Protein Sequence
(1) SbpA Protein Sequence
(1) SbpA DNA Sequence
(1) Research
(1) Site-directed Mutagenesis
(1) Device Details
(1) Pixel size calibration
(1) Electroporator
(1) 5810R Centrifuge
(1) Designing Primers
(1) Enzyme selection for BioBrick digest
(1) Restriction Digest
(1) Absorbance Labels
(1) Tutorials
(1) SbsB Protein Sequence
(1) Sap Protein Sequence
(1) Flow cell
(1) Alex Mallet
(1) Ania Labno
(1) Strains
(1) SfGS past events
(1) E. coli Genotypes
(1) Genotypes
(1) REPETITIVE STRUCTURES, MULTIPLETS, PERIODICITY ANALYSIS, SPACING ANALYSIS
(1) SbsB Amino Acid Composition

09/2005

Common: 616
Groups: 217
IGEM: 0
Protocols: 57
Materials: 1
Classes: 673
Labs: 763

Details

Group Pages

(143) Eigencluster
(26) Suggs
(15) Matsudaira
(9) Burge
(6) Ellington
(6) SfGS
(3) Tregwiki
(3) KM
(3) SB
(2) Bbf
(1) BE.109

Lab Pages

IGEM Pages

Protocol Pages

(8) Protocols
(6) Knight:TOPO TA cloning
(4) Ethanol precipitation of nucleic acids
(4) Site-directed mutagenesis
(3) Silver: Lysate for Western
(3) Acrylamide gels
(3) Knight:Agarose gel electrophoresis
(2) Sauer:Expression/purification of 35S-Met proteins
(2) DNA ligation
(2) PCR
(2) Yeast Colony PCR
(2) Colony PCR
(2) Agarose gel electrophoresis
(2) PNK Treatment of DNA Ends
(1) Silver: Time-Lapse Microscopy
(1) Silver: Colony PCR
(1) Silver: Coomassie Stain
(1) Silver: Destain
(1) Agarose Pads for Microscopy
(1) Sequencing DNA
(1) MIT:Sequencing BioBrick DNA
(1) Studier Lysate Prep
(1) Endy:Victor3 plate reader
(1) Knight:Annealing and primer extension with Klenow polymerase
(1) Restriction digest
(1) Silver: PCR

Class Pages

(84) Synthetic Biology
(11) Students for Global Sustainability Wiki
(8) The BioBricks Foundation
(3) SAVE Wiki

Material Pages

(1) T broth

Common Pages

10/2005

Common: 339
Groups: 135
IGEM: 0
Protocols: 40
Materials: 0
Classes: 268
Labs: 228

Details

Group Pages

(45) Eigencluster
(37) Suggs
(18) Matsudaira
(11) Keasling
(9) Other Stuff Re
(7) BE.109
(3) Rao
(2) MIT
(1) SfGS
(1) Wikiomics
(1) Tregwiki

Lab Pages

(110) Endy Lab
(41) Knight Lab
(31) Silver Lab
(24) Sauer Lab
(11) Keating
(4) Keasling Lab
(2) Matsudaira Lab
(2) Knight
(1) Rao Lab
(1) Grossman Lab
(1) Suggs Lab

IGEM Pages

Protocol Pages

(25) Library Generation
(2) Endy:STN Chemostat Protocol
(2) Protocols
(2) Miniprep
(2) Endy:Victor3 plate reader
(1) Endy:Pouring plates
(1) Pulse-chase protein production
(1) Knight:TOPO TA cloning
(1) Preparing chemically competent cells
(1) Endy:RNA extraction
(1) SortoStat/Operation
(1) Questions and Answers

Class Pages

(10) Synthetic Biology
(2) Students for Global Sustainability Wiki
(1) The BioBricks Foundation

Material Pages

Common Pages

(40) T7.2/gene specification
(32) Publishing Group
(30) Parts characterization
(28) BE.180
(13) AlexLabNotebook/ChrIIIRebuild/10/1/05-10/7/05
(11) Titratable control of pBAD and lac promoters in individual E. coli cells
(10) Bacterial artificial chromosomes
(9) AlexLabNotebook/ChrIIIRebuild/9/24/05-9/30/05
(9) AlexLabNotebook/ChrIIIRebuild
(9) Main Page
(9) Pam Silver
(7) AlexLabNotebook/Pheromone
(6) Lauren Ha
(6) AlexLabNotebook/YeastRebuild
(6) MIT Biological Engineering Graduate Student Board
(5) CSBI.100
(5) Publishing Group/Meeting 10-6-05
(5) TMT Thesis Project
(4) Codon Optimized for Failure
(4) OWW BioMicro Server Switch
(4) T7.2
(4) Parts characterization/Characterization approaches
(4) Useful facts
(4) Nikon TE2000 Microscope
(4) Equipment
(3) Jenny Nguyen
(3) Beta-Galactosidase Assay (A better Miller)
(3) Luis Alvarez
(3) Ved Sharma
(3) More
(3) AlexLabNotebook/ChrIIIRebuild/9/19/05-9/23/05
(3) SfGS lecture
(3) Current events
(3) Parts characterization/Measurement techniques
(3) Publishing Group/Thoughts
(3) Publishing Group/Meeting 10-4-05
(3) Chassis/System - BC Box 1
(2) Pour YPD plates
(2) Lab meetings
(2) Synthetic Genomics Study
(1) T7 Uncertainty
(1) AlexLabNotebook/YeastRebuild/9/19/05-9/23/05
(1) AlexLabNotebook/YeastRebuild/9/24/05-9/30/05
(1) AlexLabNotebook/YeastRebuild/10/1/05-10/7/05
(1) Denaturing acrylamide gel purification of nucleic acids
(1) Linder Lab
(1) Copyright
(1) Rune Linding
(1) Googlesearchheader
(1) DNA sequence design to modulate evolutionary stability
(1) Andrew Hessel
(1) Tabasco
(1) AlexLabNotebook/9/24/05-9/30/05
(1) Vectors
(1) CcdB
(1) Supergroup Mon Oct 17
(1) Counter-selectable markers
(1) September 2005
(1) Parts Characterization
(1) Computing
(1) Registry Wish List
(1) 2005 ICSB Conference
(1) AlexLabNotebook/9/19/05-9/23/05
(1) Visualization
(1) AlexLabNotebook
(1) Barry Canton
(1) Sriram Kosuri
(1) How to publish a paper
(1) AlexLabNotebook/10/1/05-10/7/05

11/2005

Common: 701
Groups: 147
IGEM: 0
Protocols: 61
Materials: 14
Classes: 185
Labs: 294

Details

Group Pages

(88) BE.109
(25) Matsudaira
(12) Bell
(8) Suggs
(4) SfGS
(3) Bcanton
(2) Tregwiki
(2) 20.109(S07)
(2) Ellington
(1) Wikiomics

Lab Pages

IGEM Pages

Protocol Pages

(20) Endy:FACS calibration with beads
(9) Protocols
(6) Site-directed mutagenesis
(5) Methods to determine the size of an object in microns
(3) Phenol/chloroform extraction
(3) Ethanol precipitation of nucleic acids
(2) Knight:Micropure EZ and Microcon purification
(2) Knight:Colony PCR
(2) Endy:Victor3 plate reader
(2) Preparing chemically competent cells
(2) In vitro transcription with T7 RNA polymerase
(1) Assaying mating
(1) High Efficiency Transformation
(1) Order a Microfluidic Chip from the Foundry
(1) Resuspension of primers
(1) Endy:Pouring plates

Class Pages

(25) Synthetic Biology
(7) SynBERC
(4) BE Board
(4) Bioinformatics Discussion Group
(2) IGEM
(1) Students for Global Sustainability Wiki

Material Pages

Common Pages

12/2005

Common: 931
Groups: 371
IGEM: 0
Protocols: 30
Materials: 19
Classes: 347
Labs: 509

Details

Group Pages

(88) BE.109
(71) Sosnick
(53) Chandra
(53) University of Chicago
(23) Labs
(19) Pan
(10) DeLong
(8) Bbf
(8) Eduardo A Silva
(6) Eigencluster
(5) Suma
(5) Dewikify
(5) MIT
(3) Keasling
(2) Bcanton
(2) Matsudaira
(2) Tregwiki
(2) Eduardo A. Silva
(2) Rao
(2) KM
(1) Fhuang
(1) Wikiomics

Lab Pages

IGEM Pages

Protocol Pages

(9) Protocols
(7) MIT:Sequencing BioBrick DNA
(4) Keating:Experimental Protocols:HPLC
(3) Endy:Victor3 plate reader
(3) Sequencing DNA
(1) Dropout plates for yeast
(1) Electrocompetent cells
(1) PNK Treatment of DNA Ends
(1) Knight:Preparing electrocompetent cells

Class Pages

Material Pages

Common Pages

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