SC
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[edit] Summary
Synthetic Complete (SC) Medium aka (CSM, CM). Defined yeast medium
[edit] Ingredients
SD Media with one or more of the following supplements:
- Adenine sulfate 20 mg/L
- Uracil 20 mg/L
- L-Tryptophan 20 mg/L
- L-Histidine-HC1 20 mg/L
- L-Arginine-HC1 20 mg/L
- L-Methionine 20 mg/L
- L-Tyrosine 30 mg/L
- L-Leucine 60 mg/L
- L-Isoleucine 30 mg/L
- L-Lysine-HC1 30 mg/L
- L-Phenylalanine 50 mg/L
- L-Glutamic acid 100 mg/L
- L-Aspartic acid 100 mg/L
- L-Valine 150 mg/L
- L-Threonine 200 mg/L
- L-Serine 400 mg/L
SC medium generally includes all supplements except one, making droput media. This allows one to select for an auxotrophic marker.
[edit] Protocol
Dropout powders can be purchased from Qbiogene or from Formedium and used to make 10X stock solutions. These solutions can be added to SD after autoclaving.
[edit] Source
Adapted From:
F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002)
or as a pdf: Getting started with yeast
