RiPS

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Ribosomes Per Second

A standard unit to measure translational activity of an mRNA molecule.

RiPS per mRNA are equivalent to the rate of the limiting step in ribosome initiation and elongation.

Study Value Note
Kennell et al. [1] 0.3 Protein mRNA-1 s-1 Measured for the lac operon in E. coli, specifically, the lacZ gene
0.06 Protein mRNA-1 s-1 Measured for the lac operon in E. coli, specifically, the lacA gene
De Smit et al. [2] 0.5 Protein mRNA-1 s-1 Assumed this value based on Tomsic et al. [3]
Jennifer Braff & Caitlin Conboy - Soup to nuts 0.039-0.55 Protein mRNA-1 s-1 Measured using BBa_I7107 and BBa_I7109 on pSB3K3 and pSB4A3.

References

  1. Kennell D and Riezman H. Transcription and translation initiation frequencies of the Escherichia coli lac operon. J Mol Biol 1977 Jul; 114(1) 1-21. pmid:409848. PubMed HubMed [Kennell]
  2. de Smit MH and van Duin J. Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA. J Mol Biol 2003 Aug 22; 331(4) 737-43. pmid:12909006. PubMed HubMed [DeSmit]
  3. Tomsic J, Vitali LA, Daviter T, Savelsbergh A, Spurio R, Striebeck P, Wintermeyer W, Rodnina MV, and Gualerzi CO. Late events of translation initiation in bacteria: a kinetic analysis. EMBO J 2000 May 2; 19(9) 2127-36. doi:10.1093/emboj/19.9.2127 pmid:10790378. PubMed HubMed [Tomsic]
All Medline abstracts: PubMed HubMed
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