T broth

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Contents

[edit] Summary

T-Broth (not to be confused with Terrific broth)

[edit] Ingredients

  1. 10 g Bacto-tryptone
  2. 5 g NaCl

[edit] Protocol

  1. Mix dry ingredients and add distilled water up to 1 Liter
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.

[edit] Source

Studier, FW Virology 39:562 (1969) Link: Pubmed

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