YPD

Summary

YPD Medium (aka YEPD). Standard growth medium for yeast.

Ingredients

• 10 g yeast extract
• 20 g peptone
• If making plates add 20 g agar
• 2% (w/v) glucose (20 g)

Protocol

1. Mix dry ingredients (except glucose) and add distilled water up to 950 mL
2. Pour into 2 L flask (or greater)
3. Autoclave (liquid cycle)
   • 250° F, 22 psi, 30 minutes
4. Add 50 mL 40% glucose

Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0 with 5M NaOH (~200µL). We usually obtain this from the kitchen.

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. 13-3