SOC

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Contents

[edit] Summary

SOC Medium. Used in Bacterial Transformation.

[edit] Ingredients

  • SOB
  • 20 mM glucose

[edit] Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

[edit] Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

[edit] Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

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