PBS

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[edit] Purpose

PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.

[edit] Recipe

1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4

To make 10x PBS,

1. Combine the following:
   • 80 g NaCl
   • 2 g KCl
   • 14.4 g Na₂HPO₄
   • 2.4 g KH₂PO₄
   • 800 mL distilled H₂O
2. Adjust pH to 7.4 with HCl
3. Add H₂O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.

[edit] Other Links

• Silver: 10x PBS
• wikipedia article and