Phleomycin

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[edit] Mode of Action

Binds and cleaves DNA

[edit] Mechanism of Resistance

The product of the Sh ble gene binds to phleomycin and inhibits its ability to cleave DNA

[edit] Usage

After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.

Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.

The stock solution is 20 ug/mL (2000X), is light blue and is stored at -20 C. It should be stable for 1 year.

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