RNA
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General pages
- Avoiding RNase contamination
- Nucleic acid structure
- Wikiomics:Alignment visualization and plotting RNA structures
Protocols
General
- Agarose gel electrophoresis
- RNA extraction
- RNA In Vitro Standards
- Probe Prep, 32P End-Labeled Probes
- In vitro transcription with T7 RNA polymerase
- Phenol/chloroform extraction
- Ethanol precipitation of nucleic acids
- Denaturing acrylamide gel purification of nucleic acids
- Digital Tools & Resources
- RNase Protection Assay
- Northern Blot, 32P End-Labeled Probes
- RNA Half-life
- Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
- RNA Quality Control
- Affymetrix Target Hybridization
- Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
- RNA electrophoresis
- Quantitating nucleic acids
- PCR techniques
- Real-time PCR
- QRT-PCR/Single tube
- QRT-PCR
- Trizol extraction for RNA and DNA
- QRT-PCR/Two tubes
- Q-PCR
- RNA extraction using trizol/tri
- Cell and tissue lysis hub
- Bioanalyzer
- RNA blot (Northern)
- Making RNA probes with T7 transcription
- SPRI bead mix
- In Situ Hybridization on Cryosectioned Tissue
Lab-specific protocols
- Endy:Northern Blot, 32P End-Labeled Probes
- Endy:RNase Protection Assay
- Endy:RNA extraction
- Knight:In vitro transcription
- Knight:RNA electrophoresis/Denaturing
- Knight:RNA electrophoresis
- Knight:RNA electrophoresis/Native
- Etchevers:Extraction ARN francais
- Sauer:RNA Purification from E. coli
- Alm:Agarose gel electrophoresis
- Wikiomics:RNA databases
- Wikiomics:RNA gene-finding
- Wikiomics:RNA phylogenetics
- Wikiomics:RNA secondary structure prediction
- Endy:Northern blot, AlkPhos end-labeled probes
- Dahlquist:DNA Microarray Protocol
- Griffin:Nested RT-PCR
- DIYbio:Notebook/Keiki Gels
- Fong:RNA gel electrophoresis/Denaturing
Materials
Resources
See Web resources#RNA and Web resources#RNA 2 and Web resources#RNA 3
