Jordan T. Detamore Week 2
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Purpose
The purpose of this assignment was to explore the genetics of different color flowers in order to determine what alleles were present. This data was then used to create a pure-breeding purple flower.
Methods/Results
Part 1
- The given flowers were Green-1, Green-2, White(W), and Red(R).
- These flowers were crossed with each other giving new colors: Blue, Yellow, Orange, Purple, Black
- The colors were self-crossed to determine what true breeding flowers could be created
- Initial Green-1 and White were true-breeding and a true breeding Red, Blue, and Yellow were discovered by self-crossing
- By crossing all the true breeding flowers, dominance was determined
- Green is dominant to white, blue and yellow
- White is recessive to Green, Yellow, Blue, and Red
- Red and Yellow have incomplete dominance producing orange
- Blue and Yellow have incomplete dominance producing green
- Red and Blue have incomplete dominance producing purple
- Since it was discovered that Red and Blue produce Purple and also that a true breeding Purple could not be created without mutation, the True Breeding Blue and Red were mutated
- Once a Purple flower was created by mutating either the Red or Blue true breeding flower, the Purple flower was self-crossed until a true breeding Purple flower was created
- Zip file with purple flower:Media:Greenhouse_with_true_P.zip
- Once a Purple flower was created by mutating either the Red or Blue true breeding flower, the Purple flower was self-crossed until a true breeding Purple flower was created
Data Tables
| Allele | Color |
|---|---|
| G | Green |
| R | Red |
| W | White |
| Y | Yellow |
| B | Blue |
| Genotype | Phenotype |
|---|---|
| WB | Blue |
| WG | Green |
| WR | Red |
| WY | Yellow |
| WW | White |
| BG | Green |
| BR | Purple |
| BY | Green |
| BB | Blue |
| GR | Black |
| GY | Green |
| GG | Green |
| RY | Orange |
| RR | Red |
| YY | Yellow |
- Genotype of Starting Strains
- Green-1: GG
- Green-2: BY
- Red: WR
- White: WW
Part II
- A mutant Orange flower was created by mutating a true-breeding Yellow flower
- The difference in Amino Acid structure between the Yellow flower and the Orange flower were observed
- The comparison shows differences many differences in Amino Acid structure
- The difference in DNA sequence between the Yellow flower and Orange flower was also observed
- The comparison shows a difference in the 46th position
Data & Files
- Zip file with purple flower:Media:Greenhouse_with_true_P.zip
- Zip with Orange flower Mutated from Yellow flower:Media:Orange from Yellow.organism.zip
Scientific Conclusion
The purpose was to manipulate the genetics of flowers using Aipotu in order to learn about the genetics of the flowers. From this information, we were challenged to make a true-breeding Purple flower. We were able to do that and manipulate the genetics of other flowers to fulfill the task.
Acknowledgements
- I worked with my lab partners Zachary T. Goldstein and Matthew Allegretti in class on this assignment
- I used Zachary T. Goldstein's wiki syntax in order to create the tables used in Part I
- I received instructions and guidance on how to complete this assignment from Dr. Dahlquist's Bioinformatics Lab
- While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.
- Jordan T. Detamore 16:23, 13 September 2016 (EDT):
References
- Week 2 Assignment created by Kam D. Dahlquist
- Aipotu (formerly Molecular Genetics Explorer) home page
Useful links
LMU Seaver College of Science and Engineering
