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Lab 5: “PCR and Fluorescence Detection of DNA”

  • Content from PCR Lab C and Lab D
    • PCR Reactions
    • Procedure
    • Data Analysis

HW: Lab Report 5 (due 04/03/18 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
10 points - Lab C content - PCR reaction report
10 points – Lab D content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter
20 points – Lab D content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary
50 points – Peer Assessment

Rubric

Initially using the micropipettes presented some small challenges. As explained in the pre-lab informational video, it is important to recognize the difference between the first and second stops when dispensing solution from the pipettes. While we were aware of this difference and used caution while handling the equipment, the most effective way to learn the proper use of micropipettes was to physically use them. While learning the proper way to operate the pipettes, some group members struggled with dispensing the full amount of solution -- not always pushing the dispense button to the second stop and sometimes releasing while the micropipette tip was still in the solution, causing air bubbles and retraction of some solution. After overcoming these learning curves, the group was able to work out an effective method of using the micropipette technology.
    EXCELLENT ACCEPTABLE WEAK*
OVERALL LAYOUT OUR TEAM section 5 pts = Original & non-offensive images; real names 3 pts = More than half original images & information 1 pt = All default green & white avatars; default text
Grammar & style 5 pts = Composition & layout perfect throughout 3 pts = Few spelling errors; images too big or small 1 pt = Many errors
PCR REACTION REPORT Summary 10 pts = Complete description, good text formatting 6 pts = Poor composition 2 pts = Missing (only default text)
FLUORIMETER PROCEDURE Webcam set-up 5 pts = Clear, easy to reproduce based on writing 3 pts = Somewhat ambiguous 2 pts = Only one sentence
Placing samples onto the fluorimeter 5 pts = Steps are thorough, clear, and easy to follow 3 pts = Run-on paragraphs 1 pt = Instructions are vague/ incomplete
DATA COLLECTION & ANALYSIS Callibration: 3 drop images, data table, 2 plots with best fit lines 10 pts = Complete 6 pts = Missing one part, somewhat unorganized 2 pts = Missing two or more parts, very unorganized
EXTRA CREDIT: gel electrophoresis +5 pts = Image of the gel; text labels on image; typed list corresponding to gel (what is in each lane) +3 pts = Image of the gel; missing labels or list +1 pts = Image of the gel, no labels, no sample list
PCR results: 2 drop images, data table, final results 10 pts = Complete 6 pts = Missing one part, somewhat unorganized 2 pts = Missing two or more parts, very unorganized

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points


Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.



Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.
Group1_W0800_L5 Group2_W0800_L5 Group3_W0800_L5 Group4_W0800_L5 Group5_W0800_L5
Group6_W0800_L5 Group7_W0800_L5 Group8_W0800_L5 Group9_W0800_L5 Group10_W0800_L5
Group11_W0800_L5 Group12_W0800_L5 Group13_W0800_L5 Group14_W0800_L5 Group15_W0800_L5
Group16_W0800_L5 Group17_W0800_L5
 
Group1_W1030_L5 Group2_W1030_L5 Group3_W1030_L5 Group4_W1030_L5 Group5_W1030_L5
Group6_W1030_L5 Group7_W1030_L5 Group8_W1030_L5 Group9_W1030_L5 Group10_W1030_L5
Group11_W1030_L5 Group12_W1030_L5 Group13_W1030_L5 Group14_W1030_L5 Group15_W1030_L5
Group16_W1030_L5 Group17_W1030_L5