Lab 5: “PCR and Fluorescence Detection of DNA”
- Content from PCR Lab C and Lab D
- PCR Reactions
- Procedure
- Data Analysis
HW: Lab Report 5 (due 04/03/18 at 11:59 pm through OpenWetWare)
Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
10 points - Lab C content - PCR reaction report
10 points – Lab D content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter
20 points – Lab D content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary
50 points – Peer Assessment
Rubric
Initially using the micropipettes presented some small challenges. As explained in the pre-lab informational video, it is important to recognize the difference between the first and second stops when dispensing solution from the pipettes. While we were aware of this difference and used caution while handling the equipment, the most effective way to learn the proper use of micropipettes was to physically use them. While learning the proper way to operate the pipettes, some group members struggled with dispensing the full amount of solution -- not always pushing the dispense button to the second stop and sometimes releasing while the micropipette tip was still in the solution, causing air bubbles and retraction of some solution. After overcoming these learning curves, the group was able to work out an effective method of using the micropipette technology.
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EXCELLENT
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ACCEPTABLE
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WEAK*
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| OVERALL LAYOUT |
OUR TEAM section |
5 pts = Original & non-offensive images; real names |
3 pts = More than half original images & information |
1 pt = All default green & white avatars; default text
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Grammar & style |
5 pts = Composition & layout perfect throughout |
3 pts = Few spelling errors; images too big or small |
1 pt = Many errors
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| PCR REACTION REPORT |
Summary |
10 pts = Complete description, good text formatting |
6 pts = Poor composition |
2 pts = Missing (only default text)
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| FLUORIMETER PROCEDURE |
Webcam set-up |
5 pts = Clear, easy to reproduce based on writing |
3 pts = Somewhat ambiguous |
2 pts = Only one sentence
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Placing samples onto the fluorimeter |
5 pts = Steps are thorough, clear, and easy to follow |
3 pts = Run-on paragraphs |
1 pt = Instructions are vague/ incomplete
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| DATA COLLECTION & ANALYSIS |
Callibration: 3 drop images, data table, 2 plots with best fit lines |
10 pts = Complete |
6 pts = Missing one part, somewhat unorganized |
2 pts = Missing two or more parts, very unorganized
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EXTRA CREDIT: gel electrophoresis |
+5 pts = Image of the gel; text labels on image; typed list corresponding to gel (what is in each lane) |
+3 pts = Image of the gel; missing labels or list |
+1 pts = Image of the gel, no labels, no sample list
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PCR results: 2 drop images, data table, final results |
10 pts = Complete |
6 pts = Missing one part, somewhat unorganized |
2 pts = Missing two or more parts, very unorganized
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Note: *Empty/ plagiarized sections or borrowed images without references receive zero points
Important Information About Grading:
- Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
- Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.
Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT!
If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.
This should open up an Edit page that is pre-populated with a lab report template code.
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BME 100 Spring 2018
