BIO254:DarkNoise

WIKIPEDIA BIO154/254: Molecular and Cellular Neurobiology

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Introduction

Noise is defined as "an unwanted signal or a disturbance in an electronic device or instrument; broadly, it is a disturbance interfering with the operation of a usually mechanical device or system". (from Merriam-Webster Online Dictionary)

Our ability to detect dim lights is limited by noise in the rod photoreceptors. These electrical events prduced in the dark which is indistinguishable from the real signal produced by light especially limit visual sensitivity at low levels of illumination. In the 1942 psychophysical experiments of Hecht, Shlaer, and Pirenne, it is showed that our dark-adapted viusal system can successfully detect the absorption of 5-7 photons. More recent reserach indicates that the ultimate limit on the accuracy of photon counting is imposed by dark noise in the retinal rods.

Both rods and cones photoreceptors respond to light by hyperpolarizing the membrane potential. This dark noise signal affects the synapse of photoreceptors only when it produced a change in membrane current that exceed a certain threshold. This threshold is a fundamental characteristic of the phototransducion process, defined as the amplitude of the spontaneous membrane current in the dark, is referred as the dark noise.

Two components of electrical dark noise

Researchers (Simon, Lamb & Hodgkin, 1975; Schwartz 1977) first reported a small random fluctuations of the membrane potential of vertebrate photoreceptors in the darkness, this so-called dark noise lessens during the response to bright steady light. Later, Baylor DA et al study this physiological noise in the visual transduction mechanism by recording membrane current from single rod outer segments in pieces of isolated toad retina, they used the suction pipette technique (see below) to examine the components of the dark noie. They found that the inward current detected in darkness showed spontaneous fluctuations which disappeared during the response to bright light. They also found that the dark noise has two components: "a continuous fluctuation of rms amplitude about 0.2pA and occational dicrete events about 1pA in size". (J. Physiol. (1980), 309, pp. 591-621)

Discrete noise

The discrete noises are rarer and larger, compared to continuous noise. The amplitude and power spectrum of the discrete events resembled those of single photon effects in the same rod. This suggests that discrete noise may arise from spontaneous activation of single rhodopsin molecules. Besides, the intervals between these occational discrete events followed the exponential distribution expected of a Poisson process with a mean rate of about one event per 50 sec (20 oC). Also, the temperature dependence of the mean frequency of occurrence of discrete events has an activation energy of 22 kcal mole-1, probably is characteristic of thermal isomerization of rhodotin. In fact, the the spontaneous isomerization of 11-cis retinal in rhodopsing is so rare (ie. on average, an isomerization time for a rhodopsin molecule is 3000 years, it doesn't happen often), makes rods capable of detecting single phtotons.

Continuous noise

The variance of the continuous noise rose linearly with the length of the outer segment drawn into the suction electord, indicating that this component is generated in the outer segment. Furthermore, the continuous noise persists when a rod is voltage-clamped, indicating that the noise arises from fluctuations in outer-segment conductance rather than fluctuations in the driving potential on the outer-segment current. Also, the frequency composition of the continuous noise can be predicted from the kinetics of the cells's response to a dim flash, suggesting continuous noise arises in the transduction cascade downstream from rhodopsin. Unlike discrete noise, the molecular mechanism of the continuous components is not known until 1996. This year, F. Rieke and D.A. Baylor investigate it in toad rods. They record the membrane current from intact and isolated rods and truncated, internally dialyzed rod outer segments. They seperate continuous noise from other noise. By selectively disablzing different elements of the phototransduction cascade, they examine their contributions to the continuous noise. Their experiments indicate that this noise is generated by spontaneous activation of cGMP phosphodiesterase (PDE) through a process that does not involve transducin.

Dark noise in rods and cones

It has been been demonstrated that cones have more noise than rods in the absence of any light. (Lamb and Simon. 1977; Schnapf et al., 1990; Schneeweis and Schnapf, 1999) For example, in primate, the amplitude of the noise is about 0.12pA in cones and is 0.03 pA in rods. Hence, rods is more sensitive than cones.

In darkness, primate rods give occasional spontaneous signal resembling reponses to single photon, just like toad. This photon-like dark noise in primate rods may result from thermal isomerization of rhodopsin. Under this assumption, plus the number of rhodopsin in a rod and rate of the noise events, the half-life of the thermal decay is 420 years. This greate stability of rhodopsin in darkness allows primate (us) to have reliable detection of very dim light.

Neither the molecular origin of the cone noise nor its functional implications are known until later the Neuron paper of 2000, "Origin and Functional Impact of Dark Noise in Retinal Cones." In this paper, they show the origin of the dark noise in salamander cones varies with cone type. Details could be found on this paper.

Major technique used in dark noise

Before the suction pipette techniques was developd in 1977, the light response of photoreceptors can only be studied by intracellular recording or by measuring extracelular voltage gradients. The disadvantage of both models is that both models can only provide information averaged over many photoreceptors.

Suction pipette techniques: To fix the problem mentioned above, and to record the elementary events of one photoreceptor, Baylor et al developed a new technique to record the membrane current of a single rod outer segment. Here is how it works: first, small pieces of retina were isolated from dark-adapted toad and kept in oxygenated toad Ringer. By using an inverted microscope and infrared image converter, they draw a single rod outer segment and fit it into a close-fitting suction electrode containing Ringer. A current sensor between the pipette and a reference electrode in a bathing solution recorded the bulk of membrane current flowing through the region of the outer segment of the rod within the pipette. Finally, a transverse slit of light (or no light=dark) is applied to the outer segment from an optical stimulator.

Major reference of this techniques:

YAU, K.-W., LAMB, T.D. & BAYLOR, D. A. (1977)

Lght-induced fluctuations in membrane current of signle toad rod outer segments. Nature. Lond. 269, 78-80

McBURNEY, R.N. & NORMANN, R.A. (1977)

Current and voltage responses from single rods in toad retina. J. Gen. Physiol. 70, 12a

References

Baylor, D. A., Matthews, G., and Yau, K.-W., (1980) J. Physiol. 309, 591-621

Hescht, Shlaer, and Pirenna, (1942) J. Gen. Physiol. 25, 819

Schwartz, E. A. J. Physiol. 272, 217-246

Simon, E. J., Lamb, T.D. & Hodgkin, A.L. (1975) Nature. Lond. 256, 661-662.

Baylor, D. A. (1987) Investigative ophthalmology & visual Science. Proctor Lecture Vol 28. 34-50.

Schnapf, J. L., Baylor, D. A. (1984) J. Physiol 357. pp. 575-602

Rieke, F., Baylor, D. A. (2000) Neuron. 26, 181-186

Rieke, F., Baylor, D. A. (1996) Biophys. J. 71, 2553-2572

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