User contributions for Kam D. Dahlquist
From OpenWetWare
Jump to navigationJump to search
26 May 2022
- 16:0016:00, 26 May 2022 diff hist −69 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: flick and spin current
- 16:0016:00, 26 May 2022 diff hist −69 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: change to flick and spin
- 15:5915:59, 26 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: change temp
25 May 2022
- 08:1908:19, 25 May 2022 diff hist +117 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: add inactivation step
- 08:1608:16, 25 May 2022 diff hist −23 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: fix parentheses
17 May 2022
- 12:4812:48, 17 May 2022 diff hist −27 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: make directions for BsmFI
- 12:3812:38, 17 May 2022 diff hist −9 Dahlquist:Lactase Persistence SNP RFLP Analysis uncomment section Tag: Manual revert
16 May 2022
- 14:5714:57, 16 May 2022 diff hist +9 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: comment out section while in progress
- 13:5913:59, 16 May 2022 diff hist +5 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 1: Restriction Digest with FaqI: add SAM to reagent order
- 13:5913:59, 16 May 2022 diff hist +32 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: change enzyme name
- 13:5713:57, 16 May 2022 diff hist +1,339 Dahlquist:Lactase Persistence SNP RFLP Analysis →Restriction Digest with FaqI: begin section for BsmFI
- 13:5613:56, 16 May 2022 diff hist +2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Overview: small change to wording
- 13:5513:55, 16 May 2022 diff hist +397 Dahlquist:Lactase Persistence SNP RFLP Analysis →Before Beginning: change to overview and outline protocol
- 13:5213:52, 16 May 2022 diff hist −1,439 Dahlquist:Lactase Persistence SNP RFLP Analysis →Restriction Digest with FaqI: delete commented out FastDigest FaqI protocol
- 13:4913:49, 16 May 2022 diff hist −30 Dahlquist:Lactase Persistence SNP RFLP Analysis →PCR Product Purification: remove reference to LOC1
- 13:4813:48, 16 May 2022 diff hist +1 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: add paren
- 13:4713:47, 16 May 2022 diff hist +18 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: add primer names
- 13:4613:46, 16 May 2022 diff hist +57 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add primers
- 13:4313:43, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: change lsb to fea
- 13:4313:43, 16 May 2022 diff hist +240 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: put in thermocycler program
- 13:3913:39, 16 May 2022 diff hist +2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: move section down a level
- 13:3913:39, 16 May 2022 diff hist +480 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: protocol for master mix
- 13:3613:36, 16 May 2022 diff hist +194 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: add master mix
- 13:3513:35, 16 May 2022 diff hist +568 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: set up reactions
- 13:3113:31, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using PHUSION Taq: lowecase phusion
- 13:3113:31, 16 May 2022 diff hist +130 Dahlquist:Lactase Persistence SNP RFLP Analysis →Phusion Polymerase: add link and information for phusion taq
- 13:2813:28, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →PHUSION polymerase: drop caps
- 13:2713:27, 16 May 2022 diff hist −59 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: remove reference to LOC1
- 13:2213:22, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: change building designation
- 13:2113:21, 16 May 2022 diff hist +38 Dahlquist:Lactase Persistence SNP RFLP Analysis →Standard Taq: add section for PHUSION
- 13:2113:21, 16 May 2022 diff hist +285 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reagents and Supplies: information about standard Taq
- 12:4912:49, 16 May 2022 diff hist +83 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add comment about 25 uL volume reactions
- 12:4612:46, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: fixed numbering
- 12:4612:46, 16 May 2022 diff hist +2 m Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: mix > mixed
- 12:4612:46, 16 May 2022 diff hist −230 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: removed LOC1 positive control from protocol
- 12:4512:45, 16 May 2022 diff hist −373 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add CT positive control
- 12:4312:43, 16 May 2022 diff hist +39 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: add heading for PHUSION Taq
- 12:4312:43, 16 May 2022 diff hist +29 Dahlquist:Lactase Persistence SNP RFLP Analysis →PCR: change to option 1, PCR using standard Taq
- 12:4212:42, 16 May 2022 diff hist −2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect Restriction Fragments: raise level of section
- 12:4112:41, 16 May 2022 diff hist −2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: moved protocol one level up
- 12:2212:22, 16 May 2022 diff hist +48 Dahlquist:Lactase Persistence SNP RFLP Analysis →BsmFI Restriction Enzyme: note about cut sites
- 12:2112:21, 16 May 2022 diff hist +355 Dahlquist:Lactase Persistence SNP RFLP Analysis breaking out primer and restriction digest information
- 12:1412:14, 16 May 2022 diff hist +23 Dahlquist:Lactase Persistence SNP RFLP Analysis move link to DNA extraction protocols
- 12:1212:12, 16 May 2022 diff hist +127 Dahlquist:DNA Extraction from Human Hair add link to RFLP protocol current
- 12:1012:10, 16 May 2022 diff hist +107 Dahlquist:DNA Extraction from Human Hair →Chelex extraction: clarify to vortex chelex before pipetting
- 12:0912:09, 16 May 2022 diff hist +7 Dahlquist:DNA Extraction from Human Hair →10% Chelex: clarified note
21 February 2022
- 15:1715:17, 21 February 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: fix Taq
- 15:1615:16, 21 February 2022 diff hist +11 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: fix alignment
14 February 2022
- 11:3711:37, 14 February 2022 diff hist +60 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: link to ladder website
- 11:3611:36, 14 February 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: fix program name