User contributions for Kam D. Dahlquist
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- 13:48, 17 May 2022 diff hist −27 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: make directions for BsmFI current
- 13:38, 17 May 2022 diff hist −9 Dahlquist:Lactase Persistence SNP RFLP Analysis uncomment section Tag: Manual revert
- 15:57, 16 May 2022 diff hist +9 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: comment out section while in progress
- 14:59, 16 May 2022 diff hist +5 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 1: Restriction Digest with FaqI: add SAM to reagent order
- 14:59, 16 May 2022 diff hist +32 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: Restriction Digest with BsmFI: change enzyme name
- 14:57, 16 May 2022 diff hist +1,339 Dahlquist:Lactase Persistence SNP RFLP Analysis →Restriction Digest with FaqI: begin section for BsmFI
- 14:56, 16 May 2022 diff hist +2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Overview: small change to wording
- 14:55, 16 May 2022 diff hist +397 Dahlquist:Lactase Persistence SNP RFLP Analysis →Before Beginning: change to overview and outline protocol
- 14:52, 16 May 2022 diff hist −1,439 Dahlquist:Lactase Persistence SNP RFLP Analysis →Restriction Digest with FaqI: delete commented out FastDigest FaqI protocol
- 14:49, 16 May 2022 diff hist −30 Dahlquist:Lactase Persistence SNP RFLP Analysis →PCR Product Purification: remove reference to LOC1
- 14:48, 16 May 2022 diff hist +1 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: add paren
- 14:47, 16 May 2022 diff hist +18 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: add primer names
- 14:46, 16 May 2022 diff hist +57 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add primers
- 14:43, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: change lsb to fea
- 14:43, 16 May 2022 diff hist +240 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: put in thermocycler program
- 14:39, 16 May 2022 diff hist +2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: move section down a level
- 14:39, 16 May 2022 diff hist +480 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: protocol for master mix
- 14:36, 16 May 2022 diff hist +194 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: add master mix
- 14:35, 16 May 2022 diff hist +568 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using Phusion Taq: set up reactions
- 14:31, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Option 2: PCR using PHUSION Taq: lowecase phusion
- 14:31, 16 May 2022 diff hist +130 Dahlquist:Lactase Persistence SNP RFLP Analysis →Phusion Polymerase: add link and information for phusion taq
- 14:28, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →PHUSION polymerase: drop caps
- 14:27, 16 May 2022 diff hist −59 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: remove reference to LOC1
- 14:22, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: change building designation
- 14:21, 16 May 2022 diff hist +38 Dahlquist:Lactase Persistence SNP RFLP Analysis →Standard Taq: add section for PHUSION
- 14:21, 16 May 2022 diff hist +285 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reagents and Supplies: information about standard Taq
- 13:49, 16 May 2022 diff hist +83 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add comment about 25 uL volume reactions
- 13:46, 16 May 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: fixed numbering
- 13:46, 16 May 2022 diff hist +2 m Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: mix > mixed
- 13:46, 16 May 2022 diff hist −230 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: removed LOC1 positive control from protocol
- 13:45, 16 May 2022 diff hist −373 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: add CT positive control
- 13:43, 16 May 2022 diff hist +39 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: add heading for PHUSION Taq
- 13:43, 16 May 2022 diff hist +29 Dahlquist:Lactase Persistence SNP RFLP Analysis →PCR: change to option 1, PCR using standard Taq
- 13:42, 16 May 2022 diff hist −2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect Restriction Fragments: raise level of section
- 13:41, 16 May 2022 diff hist −2 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: moved protocol one level up
- 13:22, 16 May 2022 diff hist +48 Dahlquist:Lactase Persistence SNP RFLP Analysis →BsmFI Restriction Enzyme: note about cut sites
- 13:21, 16 May 2022 diff hist +355 Dahlquist:Lactase Persistence SNP RFLP Analysis breaking out primer and restriction digest information
- 13:14, 16 May 2022 diff hist +23 Dahlquist:Lactase Persistence SNP RFLP Analysis move link to DNA extraction protocols
- 13:12, 16 May 2022 diff hist +127 Dahlquist:DNA Extraction from Human Hair add link to RFLP protocol current
- 13:10, 16 May 2022 diff hist +107 Dahlquist:DNA Extraction from Human Hair →Chelex extraction: clarify to vortex chelex before pipetting
- 13:09, 16 May 2022 diff hist +7 Dahlquist:DNA Extraction from Human Hair →10% Chelex: clarified note
- 16:17, 21 February 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: fix Taq
- 16:16, 21 February 2022 diff hist +11 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: fix alignment
- 12:37, 14 February 2022 diff hist +60 Dahlquist:Lactase Persistence SNP RFLP Analysis →Agarose Gel to Detect PCR Products: link to ladder website
- 12:36, 14 February 2022 diff hist 0 Dahlquist:Lactase Persistence SNP RFLP Analysis →Thermocycler Program: fix program name
- 12:36, 14 February 2022 diff hist +32 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: instruction to unpause
- 12:35, 14 February 2022 diff hist +207 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: preheat and pause thermocycler
- 12:32, 14 February 2022 diff hist +47 Dahlquist:Lactase Persistence SNP RFLP Analysis →Master Mix: add instruction about turning on thermal cycler
- 10:56, 14 February 2022 diff hist +30 Dahlquist:Lactase Persistence SNP RFLP Analysis →Reactions: clarify mixture for master mix positive control
- 10:47, 14 February 2022 diff hist +20 Dahlquist:Lactase Persistence SNP RFLP Analysis →Restriction Digest with FaqI: changed 20 microliter to entirety