BIO254:Materials

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WIKIPEDIA BIO154/254: Molecular and Cellular Neurobiology

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MATERIALS WILL BE POSTED SOON!

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11 September 2024

10 September 2024

     12:14  Pan:Press diffhist +80 Taopan talk contribs
     12:11  Pan:Publications diffhist +1,199 Taopan talk contribs
     12:03  Pan:Who we are diffhist +21 Taopan talk contribs

9 September 2024

 m   18:30  Schumer lab: Commonly used workflows‎‎ 2 changes history +1,196 [Tododge‎ (2×)]
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18:27 (cur | prev) +1,213 Tododge talk contribs (→‎Variant calling from long-read data)
     12:50  UA Biophysics:Protocols:M9 (5x) 1L‎‎ 3 changes history +47 [Elizabeth Suesca‎ (3×)]
     
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     12:11  UA Biophysics:Protocols‎‎ 2 changes history −5 [Elizabeth Suesca‎ (2×)]
     
12:11 (cur | prev) 0 Elizabeth Suesca talk contribs (→‎MEDIA)
     
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     12:09  UA Biophysics:Protocols:M9 Salts (5x) 1L diffhist −5 Elizabeth Suesca talk contribs
N    12:01  UA Biophysics:Protocols:SCD Aminoacids (10x) 1L diffhist +393 Elizabeth Suesca talk contribs (Created page with "'''Specific amino acids and supplements (10X) 1L''' * 900 mL distilled water * L-Histidine: 200 mg * L-Leucine: 300 mg * L-Lysine: 300 mg * L-Methionine: 200 mg * L-Phenylalanine: 500 mg * L-Threonine: 2 g * L-Tryptophan: 200 mg * L-Tyrosine: 300 mg * L-Valine: 1.5 g * Uracil: 200 mg Fill the bottle with MilliQ until 1 L Store at 4°C Return to Protocols<br>")
N    01:14  Prepering a standard curve to quantify the total yeast‎‎ 5 changes history +375 [Insect Nutrition and Metabolism‎ (5×)]
     
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01:11 (cur | prev) +29 Insect Nutrition and Metabolism talk contribs (Created page with "Image:Standarde_curve.png")
     01:11  (Upload log) [Insect Nutrition and Metabolism‎ (2×)]
     
01:11 Insect Nutrition and Metabolism talk contribs uploaded File:Standarde curve.png
     
01:04 Insect Nutrition and Metabolism talk contribs uploaded File:PCR protocol.pdf
N    01:08  Preforming the PCR‎‎ 7 changes history +1,047 [Insect Nutrition and Metabolism‎ (7×)]
     
01:08 (cur | prev) +2 Insect Nutrition and Metabolism talk contribs Tag: Manual revert
     
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00:57 (cur | prev) +501 Insect Nutrition and Metabolism talk contribs (Created page with "===Perform qPCR with 2X Hy-SYBR power mix (using a different kit requires protocol adjustments) on a 96-well plate=== * Prepare a separate mix for each primer you are using. The SYBR mix is very viscous, so always prepare extra mix (~ x1.2) and mix well * Primers for total yeast quantification: YEASTF (5-GAGTCGAGTTGTTTGGGAATGC-3); YEASTR (5-TCTCTTTCCAAAGTTCTTTTCATCTT T-3)[https://www.dropbox.com/scl/fi/f0khsskz124gi5m7tvenu/Real-Time-Quantitative-PCR.pdf?rlkey=nd0jyn8...")
     00:06  Preparing the plate‎‎ 4 changes history +324 [Insect Nutrition and Metabolism‎ (4×)]
     
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8 September 2024

N    23:58  Preparing the plate‎‎ 2 changes history +172 [Insect Nutrition and Metabolism‎ (2×)]
     
23:58 (cur | prev) −1 Insect Nutrition and Metabolism talk contribs
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23:47 (cur | prev) +173 Insect Nutrition and Metabolism talk contribs (Created page with "* First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly. * An example for a planned plate: Usually three technical triplicates are used.")
     23:58 Upload log Insect Nutrition and Metabolism talk contribs uploaded File:PCR plate example.pdf
     23:46  Laboratory for insect nutrition and metabolomics diffhist +112 Insect Nutrition and Metabolism talk contribs

6 September 2024