BIO254:Materials
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MATERIALS WILL BE POSTED SOON!
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11 September 2024
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08:55 | CAMRI 4 changes history +319 [Lacey Berry (4×)] | |||
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08:55 (cur | prev) +4 Lacey Berry talk contribs (→NEW AND UPCOMING!!) | ||||
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08:54 (cur | prev) −2 Lacey Berry talk contribs (→NEW: Standing Slots) | ||||
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08:54 (cur | prev) +2 Lacey Berry talk contribs (→NEW: Standing Slots) | ||||
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08:52 (cur | prev) +315 Lacey Berry talk contribs (→NEW AND UPCOMING!!) |
08:52 | Upload log Lacey Berry talk contribs uploaded File:Standing Slots at CAMRI.pdf |
08:41 | CAMRI:Training diffhist +3 Lacey Berry talk contribs (→CAMRI USER TRAINING INFORMATION) |
10 September 2024
12:14 | Pan:Press diffhist +80 Taopan talk contribs |
12:11 | Pan:Publications diffhist +1,199 Taopan talk contribs |
12:03 | Pan:Who we are diffhist +21 Taopan talk contribs |
9 September 2024
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m 18:30 | Schumer lab: Commonly used workflows 2 changes history +1,196 [Tododge (2×)] | |||
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18:30 (cur | prev) −17 Tododge talk contribs (→Variant calling from long-read data) | |||
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18:27 (cur | prev) +1,213 Tododge talk contribs (→Variant calling from long-read data) |
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12:50 | UA Biophysics:Protocols:M9 (5x) 1L 3 changes history +47 [Elizabeth Suesca (3×)] | |||
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12:50 (cur | prev) +2 Elizabeth Suesca talk contribs | ||||
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12:49 (cur | prev) +22 Elizabeth Suesca talk contribs | ||||
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12:48 (cur | prev) +23 Elizabeth Suesca talk contribs |
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12:11 | UA Biophysics:Protocols 2 changes history −5 [Elizabeth Suesca (2×)] | |||
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12:11 (cur | prev) 0 Elizabeth Suesca talk contribs (→MEDIA) | ||||
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12:10 (cur | prev) −5 Elizabeth Suesca talk contribs (→MEDIA) |
12:09 | UA Biophysics:Protocols:M9 Salts (5x) 1L diffhist −5 Elizabeth Suesca talk contribs |
N 12:01 | UA Biophysics:Protocols:SCD Aminoacids (10x) 1L diffhist +393 Elizabeth Suesca talk contribs (Created page with "'''Specific amino acids and supplements (10X) 1L''' * 900 mL distilled water * L-Histidine: 200 mg * L-Leucine: 300 mg * L-Lysine: 300 mg * L-Methionine: 200 mg * L-Phenylalanine: 500 mg * L-Threonine: 2 g * L-Tryptophan: 200 mg * L-Tyrosine: 300 mg * L-Valine: 1.5 g * Uracil: 200 mg Fill the bottle with MilliQ until 1 L Store at 4°C Return to Protocols<br>") |
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N 01:14 | Prepering a standard curve to quantify the total yeast 5 changes history +375 [Insect Nutrition and Metabolism (5×)] | |||
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01:14 (cur | prev) +4 Insect Nutrition and Metabolism talk contribs | ||||
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01:13 (cur | prev) +342 Insect Nutrition and Metabolism talk contribs | ||||
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01:13 (cur | prev) −1 Insect Nutrition and Metabolism talk contribs | ||||
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01:12 (cur | prev) +1 Insect Nutrition and Metabolism talk contribs | ||||
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01:11 (cur | prev) +29 Insect Nutrition and Metabolism talk contribs (Created page with "Image:Standarde_curve.png") |
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01:11 | (Upload log) [Insect Nutrition and Metabolism (2×)] | |||
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01:11 Insect Nutrition and Metabolism talk contribs uploaded File:Standarde curve.png | ||||
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01:04 Insect Nutrition and Metabolism talk contribs uploaded File:PCR protocol.pdf |
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N 01:08 | Preforming the PCR 7 changes history +1,047 [Insect Nutrition and Metabolism (7×)] | |||
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01:08 (cur | prev) +2 Insect Nutrition and Metabolism talk contribs Tag: Manual revert | ||||
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01:07 (cur | prev) −4 Insect Nutrition and Metabolism talk contribs | ||||
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01:07 (cur | prev) +2 Insect Nutrition and Metabolism talk contribs | ||||
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01:06 (cur | prev) +27 Insect Nutrition and Metabolism talk contribs | ||||
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01:02 (cur | prev) +133 Insect Nutrition and Metabolism talk contribs | ||||
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00:59 (cur | prev) +386 Insect Nutrition and Metabolism talk contribs | ||||
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00:57 (cur | prev) +501 Insect Nutrition and Metabolism talk contribs (Created page with "===Perform qPCR with 2X Hy-SYBR power mix (using a different kit requires protocol adjustments) on a 96-well plate=== * Prepare a separate mix for each primer you are using. The SYBR mix is very viscous, so always prepare extra mix (~ x1.2) and mix well * Primers for total yeast quantification: YEASTF (5-GAGTCGAGTTGTTTGGGAATGC-3); YEASTR (5-TCTCTTTCCAAAGTTCTTTTCATCTT T-3)[https://www.dropbox.com/scl/fi/f0khsskz124gi5m7tvenu/Real-Time-Quantitative-PCR.pdf?rlkey=nd0jyn8...") |
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00:06 | Preparing the plate 4 changes history +324 [Insect Nutrition and Metabolism (4×)] | |||
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00:06 (cur | prev) +6 Insect Nutrition and Metabolism talk contribs | ||||
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00:06 (cur | prev) +285 Insect Nutrition and Metabolism talk contribs | ||||
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00:02 (cur | prev) +6 Insect Nutrition and Metabolism talk contribs | ||||
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00:01 (cur | prev) +27 Insect Nutrition and Metabolism talk contribs |
8 September 2024
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N 23:58 | Preparing the plate 2 changes history +172 [Insect Nutrition and Metabolism (2×)] | |||
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23:58 (cur | prev) −1 Insect Nutrition and Metabolism talk contribs | ||||
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23:47 (cur | prev) +173 Insect Nutrition and Metabolism talk contribs (Created page with "* First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly. * An example for a planned plate: Usually three technical triplicates are used.") |
23:58 | Upload log Insect Nutrition and Metabolism talk contribs uploaded File:PCR plate example.pdf |
23:46 | Laboratory for insect nutrition and metabolomics diffhist +112 Insect Nutrition and Metabolism talk contribs |
6 September 2024
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16:16 | Park Lab :Lab Members 3 changes history +160 [Jeewoong Lee (3×)] | |||
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16:16 (cur | prev) 0 Jeewoong Lee talk contribs (→Former Lab Members) | ||||
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16:16 (cur | prev) +161 Jeewoong Lee talk contribs (→Former Lab Members) | ||||
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16:15 (cur | prev) −1 Jeewoong Lee talk contribs (→Undergraduate Student) |
12:32 | UA Biophysics:Protocols diffhist +140 Elizabeth Suesca talk contribs (→MEDIA) |