BIO254

• BIO254 2006       

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4 June 2023

m   07:44

User:Richard J. Abbott‎ diffhist +8‎ Richard J. Abbott talk contribs (→‎Publications)

3 June 2023

			23:44		Lee:JC‎‎ 6 changes history +26‎ [Wooin Lee‎ (6×)]
					23:44 (cur | prev) +9‎ Wooin Lee talk contribs (→‎Schedule)
					23:23 (cur | prev) −10‎ Wooin Lee talk contribs (→‎Schedule)
					23:22 (cur | prev) +6‎ Wooin Lee talk contribs (→‎Schedule)
					23:21 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					23:01 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					22:53 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)

1 June 2023

			23:23		Lee:Lab Members‎‎ 4 changes history +169‎ [Wooin Lee‎ (4×)]
					23:23 (cur | prev) +164‎ Wooin Lee talk contribs (→‎Research rotation/internship students (past))
					23:21 (cur | prev) +26‎ Wooin Lee talk contribs (→‎Graduate students)
					23:20 (cur | prev) +31‎ Wooin Lee talk contribs (→‎Alumni)
					23:19 (cur | prev) −52‎ Wooin Lee talk contribs (→‎Graduate students)

17:46

BioMicroCenter:Pricing‎ diffhist −79‎ Stuart S. Levine talk contribs (→‎SHORT READ LIBRARY - RNA)

			09:04		(Upload log)‎ [Elizabeth Suesca‎ (2×)]
					09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png ‎
					08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png ‎

31 May 2023

N    14:39

UA Biophysics:Protocols:Carotenoid Extration ESP‎ diffhist +423‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")

14:24

UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer‎ diffhist +54‎ Elizabeth Suesca talk contribs

14:23

UA Biophysics:Protocols‎ diffhist −7‎ Elizabeth Suesca talk contribs (→‎BUFFERS)

			N    14:22		UA Biophysics:Protocols:Elution Buffer‎‎ 2 changes history +1,055‎ [Elizabeth Suesca‎ (2×)]
					14:22 (cur | prev) +467‎ Elizabeth Suesca talk contribs
			N		14:07 (cur | prev) +588‎ Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")

			N    14:03		UA Biophysics:Protocols:Calcein ESP‎‎ 3 changes history +1,249‎ [Elizabeth Suesca‎ (3×)]
					14:03 (cur | prev) +43‎ Elizabeth Suesca talk contribs (→‎Procedimiento:)
					14:02 (cur | prev) +63‎ Elizabeth Suesca talk contribs
			N		14:00 (cur | prev) +1,143‎ Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")

12:48

BioMicroCenter:QC‎ diffhist −421‎ Stuart S. Levine talk contribs (→‎AGILENT BIOANALYZER 2100)

			08:34		UA Biophysics:Protocols:Calcein‎‎ 2 changes history −751‎ [Elizabeth Suesca‎ (2×)]
					08:34 (cur | prev) +53‎ Elizabeth Suesca talk contribs
					08:33 (cur | prev) −804‎ Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced

N    08:31

UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP‎ diffhist +1,206‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")

06:42

UA Biophysics:Protocols:Lipid Extraction‎ diffhist −9‎ Elizabeth Suesca talk contribs

30 May 2023

23:52

Hexlab‎ diffhist +440‎ Ximiao He talk contribs

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