All public logs

Combined display of all available logs of OpenWetWare. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 13:08, 10 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Lyophilizer ESP (Created page with " thumb|right|240px '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>ANTES DE ENCENDER:</h2> #Revise que la cámara del liofilizador este alineada y seca. #Si no lo está, límpiela muy bien usando una toal...")
• 07:23, 9 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Plasma Cleaner ESP (Created page with " thumb|right|240px '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' ''' Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>FUNCIONAMIENTO CON AIRE:</h2> #Abra la válvula de la bomba de vacío y cierre la válvula de precisión. #La flecha en la válvula p...")
• 08:50, 8 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Spectrofluorometer Instructions ESP (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo.''' <h2>TURN ON THE EQUIPMENT: </h2> #Turn off computer and instrument before starting the lamp power supply. #Set the current knob of the lamp power supply completely counter-clock-wise turn th...")
• 08:45, 8 November 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Autoclave Instructions ESP (Created page with " '''Si no tiene autorización para usarlo por favor comunicarse con [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>ANTES DE ESTERILIZAR:</h2> # El material debe estar limpio. # Los objetos metálicos y pipetas deben estar envueltos en papel. # Coloque la cinta indicadora en cada frasco y dentro de...")
• 12:15, 26 June 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Lipid Extration ESP (Created page with "==Materials for 1 g of Cell== * Células SA401 pesadas y maceradas con mortero * Cloroformo * Metanol Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * Perlas de vidrio * Solución de NaCl 1.7 M (10% W/V) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Fascos de vidrio de 50 ml '''Nota:''' Preparar una mezcla de cloroformo:metanol (2:1) (20 ml de cloroformo/10 m...")
• 08:04, 1 June 2023 Elizabeth Suesca talk contribs created page File:Cloroformo.png
• 08:04, 1 June 2023 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png
• 07:25, 1 June 2023 Elizabeth Suesca talk contribs created page File:Acetato.png
• 07:25, 1 June 2023 Elizabeth Suesca talk contribs uploaded File:Acetato.png
• 13:39, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Carotenoid Extration ESP (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")
• 13:07, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Elution Buffer (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")
• 13:00, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Calcein ESP (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")
• 07:31, 31 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")
• 11:57, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Bacteria for Lipid Extraction (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")
• 11:17, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Erythromycin (Created page with "Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains. ==STOCK SOLUTION== Erythromycin is used in the biophysics lab at a 2 ug/ml. Stock solutions are at 1 mg/mL ==PROTOCOL (STORAGE)== According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 20mL of 25mg...")
• 10:58, 29 May 2023 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Calcein (Created page with "Receta para 100 ml, 50mM, 400 mOsM, pH 7,4 Materiales: Probeta 100 ml 30 ml de NaOH 3,113 g de calceina Procedimiento: En probeta 20 ml de NaOH 1M (colocque 21 ml) Disolver en el NaOH 3,113 g de calceina Mezclar 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) y HEPES 238.3 mg (para que quede a 10 mM).completar un volumen de 40 ml Agregar la mezcla a la calceina Ajustar pH con HCl 1M (2 ml) Entre HCl y NaOH se agregaron 2...")
• 05:39, 20 January 2023 Elizabeth Suesca talk contribs created page File:Desionizador Partes.jpg
• 05:39, 20 January 2023 Elizabeth Suesca talk contribs uploaded File:Desionizador Partes.jpg
• 05:38, 20 January 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Water Purification System (Created page with " '''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #Remove the cap and place your flask. #Press “DISP” #Press “DISP” (RO, destilled) o “FLUSH” (UP, deionized) to choose the water quality. Ensure that the pure water at lea...")
• 06:53, 19 January 2023 Elizabeth Suesca talk contribs created page File:Desionizador.jpg
• 06:53, 19 January 2023 Elizabeth Suesca talk contribs uploaded File:Desionizador.jpg
• 06:48, 19 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Incubadora refrig.jpg
• 06:45, 19 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Incubadora.jpg
• 07:11, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Syringe.jpg
• 07:10, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Plasma.jpg
• 07:09, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Spectrofluorimeter.jpg
• 07:05, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Horno.jpg
• 06:42, 18 January 2023 Elizabeth Suesca talk contribs created page File:Espectrofotometro Partes.jpg
• 06:42, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:Espectrofotometro Partes.jpg
• 06:34, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Hot.jpg
• 06:33, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Centrifuga.jpg
• 06:29, 18 January 2023 Elizabeth Suesca talk contribs created page File:UA NanoDrop.JPG
• 06:29, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:UA NanoDrop.JPG
• 06:27, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Sonicador.jpg
• 06:21, 18 January 2023 Elizabeth Suesca talk contribs created page File:Sonicador-partes.jpg
• 06:21, 18 January 2023 Elizabeth Suesca talk contribs uploaded File:Sonicador-partes.jpg
• 06:15, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Logichepamain.jpg
• 06:12, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Balanza-analitica-xb-220a-precisa.jpg
• 06:05, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Liofilizador.jpg
• 05:56, 18 January 2023 Elizabeth Suesca talk contribs uploaded a new version of File:Autoclave.jpg
• 13:18, 17 January 2023 Elizabeth Suesca talk contribs created page UA Biophysics: Spectrophotometer (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #The cell holder have to be empty before turning on the instrument. #Turn on the instrument using the power switch on the back panel. #Wait almost 20 min before using it. Spectrometer...")
• 07:28, 22 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Centrifuge Primo R (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Quick Start</h2> #Turn on the mains switch on the back of the instrument. #After internal check, the display switches to the actual values. #Set temperature, velocity, and time using the UP/DOWN KEY...")
• 06:26, 17 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Syringe Pump (Created page with "[https://www.harvardapparatus.com/media/manuals/Product%20Manuals/11%20Elite%20Manual_5420-002REV1.0.pdf Operator´s Manual] Return to equipment")
• 05:30, 17 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: NanoDrop (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Quick Start</h2> #Double-click the software icon and select the software application of interest from the right pane. Select “Add to report” prior to a measurement to save the sample data to a wor...")
• 11:26, 16 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Sonicador (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Functions</h2> '''MAIN POWER SWICH:''' This switch is located on the back of the unit, next to the power cord receptacle. '''ON/STANDBY:''' When the Main Power switch on the rear panel is in the ON...")
• 06:22, 16 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Cabinet Biosafety (Created page with " '''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>Start-up</h2> #Slowly raise the sash until the bottom of the sash aligns with the sash indicator decal located on the left side of the work area. #Check the air grilles for obstructions. The display...")
• 12:10, 15 February 2022 Elizabeth Suesca talk contribs created page UA Biophysics: Analytical Balance Instructions (Created page with " thumb|right|240px '''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' '''For more information please visit the following link [https://www.youtube.com/watch?v=CNahxRg9QEk]''' Return to equipment")
• 08:55, 1 October 2021 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Buffer Freezing C. elegans Strains (Created page with "'''Freezing Solution (1L)''' * 5.8g NaCl * 50mL 1M KH2PO4 (pH 6.0) * 240mL glycerol * 710mL ddH2O #Bottle and autoclave #Add 30μL 1M MgSO4 per 100mL of solution")
• 12:19, 29 September 2021 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Freezing C. elegans Strains (Created page with "'''Reagents Needed:''' * M9 (common stock) * Freezing solution '''Procedure:''' Make sure you begin with healthy, non-contaminated, non-starved worms of the correct phenotyp...")
• 11:05, 29 September 2021 Elizabeth Suesca talk contribs created page UA Biophysics:Protocols:Siembra de nemátodos (Created page with "Return to Protocols<br>")