Pelling:Protocols

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Bacterial Culture Protocols and Buffers LB Broth Kanamycin and Ampicillin Making Agar Plates Bacterial Cell Culture - Thawing, Plating and Inoculation Preparing and Transforming Chemically Competent Bacteria Protozoa Culture Protocols and Buffers Protozoan Media Formulations Cell Sources Paramecium caudatum Culture Amoeba proteus Culture General Literature on Protozoan Culture Mammalian Cell Culture Protocols and Buffers Aliquoting FBS, P/S, TE DMEM Recipe PBS Buffer Mammalian Cell Culture - Thawing, Passage, Freeze Gelatine Hydrogels PDMS Gels and Functionalization DNA Isolation and Transfections Extracting DNA from Filter Paper Plasmid DNA Isolation General Transfection Procedure with Lipofectamine 2000 Creating a Stable Line Troubleshooting Transfections Staining Procedures and Buffers Sodium Azide Recipe PBS Buffer Paraformaldehyde Buffer Recipe Permeabilizing Buffer Recipe Wash Buffer Recipe General Triple Staining Procedure Methanol Fix and Permeabilize Protocol Basic Microscope Operation Atomic Force Microscope JPK Offline Software and Manuals Confocal Microscope Phase/Epi-Florescence Microscope Lab Maintenance Autoclave Procedure Liquid Nitrogen Tissue Culture Incubator, CO2 and Maintenance Sterilizing Pasteur Pipettes