Pelling:Protocols/Paramecium caudatum Culture

<owwmenu font="arial, helvetica, sans-serif" bold="1"

       color="black" bgcolor="white" hovercolor="black"
       bghovercolor="gray" topfontsize="10" fontSize="8" pagewidth="930"
       image="Bishop.jpg">
   Home=Pelling
   Protocols=Pelling:Protocols
   Lab Supplies=Pelling:Lab Supplies
   Lab Safety and MSDS's=Pelling:Lab Safety

</owwmenu>

Media In a 500mL Erlenmeyer flask add 50mL of Hay Media and 3 boiled wheat grains. Add 150mL of dH2O Optional: Add 150μL of CH or 150μL of PJ Solution Let stand at room temperature 24 hours. Use a small piece of tin foil to cover the top of the flask (not tightly, you want air to flow). The next day inoculate with P. caudatum and keep at room temperature in the dark. Subculture When you have a healthy culture going (about 1-2 weeks after inoculation) you can subculture as necessary in fresh media and new wheat grains. Remember to prepare the media the day beforehand. Notes Cells collect on the surface and throughout the media.