Pelling:Protocols/Extracting DNA from Filter Paper

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Plasmids are often shipped from one lab to another on filter paper. When receiving a plasmid from another lab you will typically get a piece of filter paper with a circle drawn on it in pencil. Usually 1-2μL of plasmid DNA is deposited inside the circle, to extract the DNA: Cut out the circle containing the DNA (do not include the pencil marks). Place in a micro-centrifuge tube with 100μL of 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 buffer (TE Buffer). You can also use dH2O or 10mM Tris buffer. Briefly vortex and let stand for 15min (or overnight in fridge). Use 1-10μL (usually 2μL is good) to transform competent E. coli. Keep tube in the fridge until you are sure that the DNA has been isolated and you are happy with the transformation, transfection and expression of the plasmid.