From OpenWetWare
Jump to: navigation, search

<owwmenu font="arial, helvetica, sans-serif" bold="1"

       color="black" bgcolor="white" hovercolor="black"
       bghovercolor="gray" topfontsize="10" fontSize="8" pagewidth="930"
   Lab Supplies=Pelling:Lab Supplies
   Lab Safety and MSDS's=Pelling:Lab Safety


LB Broth

  1. To make LB Broth Media, mix 10g of LB Broth with 400mL dH2O in a duran bottle.
  2. Autoclave for 20min (wet cycle) and only use once cooled to room temperature. Make sure to close lids tightly after autoclaving.
  3. Store in the fridge at 4°C. Always use sterile pipette tips and techniques to avoid contamination. Discard immediately if any growth occurs or if the media appears cloudy.