Pelling:Protocols/LB

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LB Broth

  1. To make LB Broth Media, mix 10g of LB Broth with 400mL dH2O in a duran bottle.
  2. Autoclave for 20min (wet cycle) and only use once cooled to room temperature. Make sure to close lids tightly after autoclaving.
  3. Store in the fridge at 4°C. Always use sterile pipette tips and techniques to avoid contamination. Discard immediately if any growth occurs or if the media appears cloudy.