Pelling:Protocols/DMEM

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Basic DMEM Media Formulation (10% FBS, 1% P/S) - Complete Thaw 5ml P/S and 50ml FBS in 37°C water bath. Obtain a new 500ml bottle of DMEM (with l-Glutamine) from the fridge. Remove 55ml from DMEM using a 25ml pipette into a waste beaker. Using the same tip, dispense 50 ml of FBS in the DMEM. Use a clean 5ml pipette to withdraw 5ml of P/S and dispense into the DMEM. Final formulation: 50ml FBS + 5ml P/S + 445ml DMEM