Mpaniag1 Week 5
Purpose
The purpose of this activity is to analyze the data from the paper, 'Patterns of HIV-1 evolution in individuals with differing rates of CD4 T cell decline', and discover similarities and differences between the sequences from a subset of subjects I have chosen
Methods and Results Activity 1
Part 1
- Followed Link on Week 5 assignment page to PubMed's website [1]
- In the search bar, searched the title of the paper of interest, in this case, Patterns of HIV-1 evolution in individuals with differing rates of CD4 T cell decline
- Another way you can search for the paper of interest is searching by the author's name or subject
- Clicking on the paper will take you to the abstract of the paper itself and the figures from the paper, on the side bar PubMed has two links for full text of the article, similar articles, all the articles that cited this paper, related information in MedGen, nucleotide sequences, protein sequences, taxonomy, references from this paper, search details, and recent activity
- Clicked on Nucleotide link in related information [2]
Part 2
- Clicked on link to HIV-1 subject 1 visit 1 clone 9 [3]
- Ascension #-AF016768
- Subject-1
- How to Find Source-Under features, the sub header, source, leads you to the information about the subject
- Downloaded five sequences, subject 1, visit 1 clone 9; subject 2, visit 4 clone 2; subject 3, visit 5 clone 6; subject 3, visit 4 clone 8; subject 12, visit 4 clone 1
- Clicked send to-->complete record-->file-->FASTA, create file (give it a descriptive name so it is easy to find later)
- Opened the files in notebook, copy and paste them to the webpage
- Sequence for Subject 1, Visit 1 clone 9
- >AF016768.2 HIV-1 subject 1 visit 1 clone 9 from USA, envelope glycoprotein V3 region (env) gene, partial cds
GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACGGCTGAATGAATCTG TAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACATATAGGACCAGGTAGAGCATT TTATACAACAGGAGACATAATAGGAGATATAAGACAAGCATATTGTAACATTAGTAGAGCAGAATGGAAT AACACTTTAAAACAGATAGTTATAAAATTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACT CCTCA
- Sequence for HIV-1 subject 2, visit 4 clone 2
- >AF016818.2 HIV-1 subject 2, visit 4 clone 2 from USA, envelope glycoprotein V3 region (env) gene, partial cds
GAGGTAGTAATTAGATCTGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTAAATGAATCTG TAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACATATAAGACCAGGTAGAGCATT TTATACAACAAGAGACATAATAAGAGATATAAGACAAGCATATTGTAACATTAGTAGAGCAGAATGGAAT AACACTTTAAAACAGATAGTTATAAAATTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACT CCTCA
- Sequence from HIV-1 subject 3, visit 5 clone 6
- >AF089137.1 HIV-1 isolate S3V5-6 from USA envelope glycoprotein (env) gene, partial cds
GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTGAATGAAACTG TAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACTCTAGGACCAGGCAGAGTATA CTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCACATTGTAACCTTAGTAGAGCAGGTTGGACT AACACTTTAGAAAGGATAGCTATAAAATTAAGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAAT CCTCA
- Sequence from HIV-1 subject 3, visit 4 clone 8
- >AF089130.1 HIV-1 isolate S3V4-8 from USA envelope glycoprotein (env) gene, partial cds
GATGTAGTAATTAGATCCGCCAATTTCGCGGACAATGCTAAAACCATACTAGTACAGCTGAATGAAACTG TAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACTCTAGGACCAGGCAAAGTATA CTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCACATTGTAACCTTAGTAGAGCAGGTTGGAAT AACACTTTAAAAAGGATAGCTATAAAATTAAGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAAT CCTCA
- Sequence from HIV-1 subject 12, visit 4 clone 1
- >AF089542.1 HIV-1 isolate S12V4-1 from USA envelope glycoprotein (env) gene, partial cds
GAGGTAGTAATTAGATCCAAGAATTTCACGGATAATGCTAAAATCATAATAGTACAGCTAAATGAGACTG TAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACCTATAGGACCAGGCAGAGCATT TTATACAACAGGAGAAATAATAGGAGATATAAGACAAGCACATTGTACCCTTAGTAGAGCAAAATGGAAT GAAACTTTAAAACAGATAGTTATAAAATTAAAAGAACAATTTAGGAATAAGACAATAGTCTTTAGTCCAT CCTCA
Part 3
- Went to Phylogeny Website [4]
- Clicked on phylogeny analysis-->One click
- Copied and pasted the text files for each sequence used in Part 2
- Clicked Submit
- Clicked on Tab, Alignment
- Clicked on outputs, chose the clustal format option
- Copied and pasted that data below
- For the phylogenetic tree, went back to the page that shows up after you click submit, chose Tree Rendering tab this time
- Used Snip tool to take a screenshot of the tree produced, and saved it with a descriptive name
- Uploaded image to wiki page
- Compared the sequences' differences to the makeup of the tree
Alignment
AF089130.1 GATGTAGTAATTAGATCCGCCAATTTCGCGGACAATGCTAAAACCATACTAGTACAGCTG AF089137.1 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG AF089542.1 GAGGTAGTAATTAGATCCAAGAATTTCACGGATAATGCTAAAATCATAATAGTACAGCTA AF016818.2 GAGGTAGTAATTAGATCTGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTA AF016768.2 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACGGCTG ** ************** ****** ** * ********** **** ****** ***
AF089130.1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT AF089137.1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT AF089542.1 AATGAGACTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACCT AF016818.2 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT AF016768.2 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT ***** ****** *** *************** ****************** ** *
AF089130.1 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA AF089137.1 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA AF089542.1 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGAAATAATAGGAGATATAAGACAAGCA AF016818.2 ATAAGACCAGGTAGAGCATTTTATACAACAAGAGACATAATAAGAGATATAAGACAAGCA AF016768.2 ATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA ** ******* * ** ** ********* ** * ****** *********** *****
AF089130.1 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA AF089137.1 CATTGTAACCTTAGTAGAGCAGGTTGGACTAACACTTTAGAAAGGATAGCTATAAAATTA AF089542.1 CATTGTACCCTTAGTAGAGCAAAATGGAATGAAACTTTAAAACAGATAGTTATAAAATTA AF016818.2 TATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAATTA AF016768.2 TATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAATTA ****** * *********** **** * * ****** ** ***** **********
AF089130.1 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA AF089137.1 AGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAATCCTCA AF089542.1 AAAGAACAATTTAGGAATAAGACAATAGTCTTTAGTCCATCCTCA AF016818.2 AGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA AF016768.2 AGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA * ****** *** ***** ************* ** ******
Figure 1:Phylogenetic Tree-Activity 1, Part 3
Methods and Results Activity 2
Methods Activity 2 Part 1
- Downloaded two files with the different subjects and their clones
- Chose Subjects 1,3,5, and 7, and clones 1,2, and 3 for each
- Generated a multiple sequence alignment with the 12 sequences with Phylogeny website [5]
- Clicked on Phylogeny Analysis, then One Click
- Pasted the sequences in-->clicked submit
- Clicked on Alignment tab
- Copied and pasted data below
- Generated a phylogenetic tree from the sequences chosen
- Clicked on Tree Rendering tab
- Used snip tool
- Saved with a descriptive name
- Uploaded to wiki
Results Part 1
Alignment
S3V1-3 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATACTAGTACAGCTG S3V1-1 GATGTAGTAATTAGATCCGCCAATTTCTCGGACAATGCTAAAACCATACTAGTACAGCTG S3V1-2 GATGTAGTAATTAGATCCGCCAATTTCTCGGACAATGCTAAAACCATACTAGTACAGCTG S7V1-3 GAGATAGTAATTAGATCTGCCAATCTCTCGGACAATGCTAAGACCATAATAGTACAGCTG S7V1-1 GAGATAGTAATTAGATCTGCCAATTTCACGGACAATACTAAGACCATAATAGTACAGCTG S7V1-2 GAGGTAGTAATTAGATCTGCCAATTTCACGGACAATGCTAAAACCATAATAGTACAGCTG S5V1-3 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-1 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAGTAGTACATCTA S5V1-2 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S1V1-2 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-1 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-3 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG ** ************* *** ** ** ***** **** * **** ******* **
S3V1-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGAGTAACT S3V1-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V1-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S7V1-3 AATGCACCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGGAAAAGTATATCT S7V1-1 AATGTATCTGTAGAAATTAATTGTACGAGACCCAACAACAATACAAGAAAAAGTATACCT S7V1-2 AATGCACCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACTT S5V1-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S1V1-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT S1V1-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT S1V1-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT **** * ****** *** ******** ****** ************ * *** ** *
S3V1-3 CTAGGACCAGGCAAAGTATACTACACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-1 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-2 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S7V1-3 ATAGGACCAGGGAGAGCATTTTATGCTACAGGAGAAATAATAGGAAATATAAGACAAGCA S7V1-1 ATAGGACCAGGGAGAGCATTTTATGCTACAGGAGAAATAATAGGGAATATAAGACAAGCA S7V1-2 ATAGGACCAGGGAGAGCATTTTATGCTACAGGAGAAATAATAGGAAATATAAGACAAGCA S5V1-3 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGACATAAGACAAGCA S5V1-1 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-2 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S1V1-2 ATAAGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAAGAGATATAAGACAAGCA S1V1-1 ATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S1V1-3 ATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA ** ******* * ** ** ** * ****** * ****** * * ****** *****
S3V1-3 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V1-1 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V1-2 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S7V1-3 CATTGTAACATTAATAGAGCAAAATGGAATAACACTTTAAAACAGATAGCTACAAAATTA S7V1-1 CATTGTAACATTAGTAGAGCAAAATGGAATAACACTTTAAAACAGATAGCTACAAAATTA S7V1-2 CATTGTAACATTAGTAGAGCAAAATGGAATAACACTTTAAAACAGATAGCTACAAAATTA S5V1-3 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-1 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S1V1-2 TATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAATTA S1V1-1 TATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAATTA S1V1-3 TATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAATTA ******** *** * ** * * *** ** * ********* ***** ** *******
S3V1-3 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V1-1 AGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAATCCTCA S3V1-2 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S7V1-3 AGAAAACAATTTGAGAATAAAACAATAGTCTTTAATCAATCCTCA S7V1-1 AGAAAACAATTTGAGAATAAAACAATAGTCTTTAATCAATCCTCA S7V1-2 AGAAAACAATTTGAGAATAAAACAATAGTCTTTAATCAATCATCA S5V1-3 AGAGAACAATTTGGGAATAAAACAATAGTATTTAATTCATCCGCA S5V1-1 AGAGAACAATTTGGGAATAAAACAATAGTATTTAATCCATCCTCA S5V1-2 AGAGAACAATTTGGGAATAAAACAATAGTATTTAATCCATCCTCA S1V1-2 AGAGAACACTTTAGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-1 AGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCTTCA S1V1-3 AGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA *** **** *** ************** ****** ** **
Figure 2-Phylogenetic Tree-Activity 2, Part 1
Subject | Clone # |
1 | 1 |
2 | |
3 | |
3 | 1 |
2 | |
3 | |
5 | 1 |
2 | |
3 | |
7 | 1 |
2 | |
3 |
- Do the clones from each subject cluster together?
- For this exercise I chose subjects 1,3,5,and 7 and the clones 1,2, and 3 for each. The tree shows that the clones from each subject are clustered together.
- Do some subjects' clones show more diversity than others?
- Clones from Subjects 7 and 3 are more diverse because there is more splitting, for 7 clone 2 is split off from 3 and 1, and for 3 clone 1 is split off from 2 and 3
- Do some of the subjects cluster together?
- No, each subject and its clones have their own clusters.
- Write a brief description of your tree and how you interpret the clustering pattern with respect to the similarities and potential evolutionary relationships between subjects' HIV sequences.
- In the tree that was generated from my chosen Subjects, each subject's clones clustered together, the only difference was subject 3 and 7 had one clone from their set branch differently. Based on this data it can be hypothesized that each subject's clones are similar with minute differences, starting from a similar place. The potential evolutionary relationship is hypothesized that HIV evolves similarly even within different subjects.
Methods for Activity 2 Part 2
- Go to BedRock [6] and select a subject
- Copied and pasted all of subject's clones from their recorded visits into Phylogeny Website [7]
- Clicked on tab labeled Tree Rendering, took screenshot using snipping tool, uploaded image to wiki
- Clicked on tab labeled Alignment, copied and pasted sequences into wiki
- Counted the number of clones and visits per subject
- Counted the number of different nucleotides (S value)
- Used a harmonic sum calculator [8], and inserted the Number of clones for the number of terms and then put S value over that number
Results Part 2
Subject 1
Alignment Subject 1
S1V5-2 GAGGTAGTAGTTAGATCCGAAAATTTCACGAACAATGCTAAAACCATAATAGTACAGCTG S1V5-5 GAGGTAGTAGTTAGATCCGAAAATTTCACGAACAATGCTAAAACCATAATAGTACAGCTG S1V5-1 GAGGTAGTAGTTAGATCCGAAAATTTCACAAACAATGCTAAAACCATAATAGTACAGCTG S1V5-12 GAGGTAGTAATTAGATCTGAAAATTTCACGAACAATGCTAAAACCATAATAGTACAGCTG S1V5-13 GAGGTAGTAGTTAGGTCTGAAAATTTCACGAACAATGCTAAAACCATAATAGTACAGCTG S1V1-13 GAGGTAGTAATTAGATCTGTCAATTTCACGGACAATGCTAAAACCATAATAGTACAGCTG S1V1-4 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-6 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAGATCATAATAGTACAGCTG S1V2-11 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-5 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-5 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-12 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-2 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-10 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-6 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-10 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-7 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-8 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-9 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-12 GAGGTAGTAATTAGATCTGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-11 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-3 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-9 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-7 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-1 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V1-1 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-14 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-15 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-4 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAGATCATAATAGTACAGCTG S1V2-8 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-13 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V2-16 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAGTCATAATAGTACAGCTG S1V2-2 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAGTCATAATAGTACAGCTG S1V2-3 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAGTCATAATAGTACAGCTG S1V5-8 GAGGTAGTAGTTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACATCTG S1V5-10 GAGGTAGTAATTAGATCCGAAAATTTCACGAACAATGCTAAAATCATAATAGTACATCTG S1V5-11 GAGGTAGTAGTTAGATCCGAAAATTTCACGAACAATGCTAAAACCATAATAGTACAGCTG S1V5-3 GAGGTAGTAATTAGATCCGAAAATATCGCGAACAATGCTAAAATCATAATAGTACAGCTG S1V5-4 GAGGTAGTAATTAGATCTGAAAATATCACGAACAATGCTAAAATCATAATAGTACAGCTG S1V5-6 GAGGTAGTAATTAGATCTGAAAATATCATGAACAATGCTAAAATCATAATAGTACAGCTG S1V5-9 GAGGTAGTAATTAGATCTGAAAATATCATGAACAATGCTAAAATCATAATAGTACAGCTG S1V5-7 GAGGTAGTAATTAGATCTGAAAATATCATGAACAATGCTAAAATCATAATAGTACAGCTG ********* **** ** * *** ** ********** ************ ***
S1V5-2 AATAAATCTGTAAACATTAGTTGTATAAGACCCAACAACAATACAAGAAGAAGTATA--- S1V5-5 AATAAATCTGTAAACATTAGTTGTATGAGACCCAACAACAATACAAGAAGAAGTATA--- S1V5-1 AATAAATCTGTAAACATTAGTTGTATGAGACCCAACAACAATACAAGAAAAAGTATA--- S1V5-12 AATAAACCTGTAAACATTAGTTGTATGAGACCCAACAACAATACAAG---AAGTATA--- S1V5-13 AATAAATCTGTAAACATTAGTTGTATGAGACCCAACAACAATACAAGAAGAAGTATA--- S1V1-13 AATACATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-4 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-6 AATAAATCTATAGAAATTAATTGTACAAGACCCAACAACAATACGAGAAAAAGTATA--- S1V2-11 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGGAAAAGTATA--- S1V1-5 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-5 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-12 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-10 GATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-6 AATGAATCTGTAGAAATTAACTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-10 AATGAATCTGTAGAAATTAACTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-7 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-8 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-9 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-12 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-11 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-9 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-7 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-1 AATAAATCTATAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V1-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-14 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-15 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-4 AATAAATCTATAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-8 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-13 AATGAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-16 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-2 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V2-3 AATAAATCTGTAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATA--- S1V5-8 AATGAATCTGTAGGAATTAATTGTACAAGACCCAACAACAATATAAAAAAAAGAATAATA S1V5-10 AATGAATCTGTAGGAATTAATTGTACAAGACCCAACAACAATATAAAAAAAAGAATAATA S1V5-11 AATAAATCTGTAAACATTAGTTGTATGAGACCCAACAACAATATAAAACAAAGAATAATG S1V5-3 AATGAATCTGTAGCAATTAATTGTACAAGACCCAACAACAATATAAAACAAAGAATAATA S1V5-4 AATGAATCTGTAGCAATTAGTTGTACAAGACCCAACAACAATATAAAACAAAGAATAATG S1V5-6 AATGAATCTGTAGCAATTAATTGTACAAGACCCAACAACAATATAAAACAAAGAATAATG S1V5-9 AATGAATCTGTAGCAATTAATTGTACAAGACCCAACAACAATATAAAACAAAGAATAATG S1V5-7 AATGAATCTGTAGCAATTAATTGTACAAGACCCAACAACAATATAAAACAAAGAATAATG ** * ** ** **** **** **************** * *** ***
S1V5-2 AATATAGGACCAGGTAGAGCATTTTATACAACAGGAGAAATAATAGGAGATATAAGACCA S1V5-5 AATATAGGACCAGGTAGAGCATTTTATACAACAGGAGAAATAATAGGAGATATAAGACAA S1V5-1 AATATAGGACCAGGTAGAGCATTTTATACAACAGAAGACATAATAGGAGATATAAAACCA S1V5-12 AATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V5-13 AATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-13 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-4 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-6 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-11 CATATAGGACCAGGTAGGGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-5 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACCA S1V2-5 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-12 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-2 CATATAAGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAAGAGATATAAGACAA S1V1-10 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAG S1V1-6 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-10 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-7 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAG S1V1-8 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAG S1V1-9 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAG S1V1-12 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAG S1V1-11 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-3 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-9 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-7 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-1 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V1-1 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-14 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACCA S1V2-15 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-4 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-8 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-13 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-16 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V2-2 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACCA S1V2-3 CATATAGGACCAGGTAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACCA S1V5-8 CATATAGGACCAGGTAGAGCATTTTATACAA---GAGAC---AAAGGAGATATAAGACAA S1V5-10 CATATAGGACCAGGTAGAGCATTTTATACAA---GAGAC---AAAGGAGATATAAGACAA S1V5-11 CATATAGGACCAGGTAGAGCATTTTATACAA---AAGACATAACAGGGGATATAAGACAA S1V5-3 CATATAGGACCAGGTAGACCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAA S1V5-4 CATATAAGACCAGGTAGAGCATTTTATACAA---AAGACATAACAGAAGATATAAGACAA S1V5-6 CATATAGGACCAGGTAGAGCATTTTATACCA---AAGACATAACAGGGGATATAAGACAA S1V5-9 CATATAGGACCAGGTAGAGCATTTTATACAA---AAGACATAACAGGGGATATAAGACAA S1V5-7 CATATAGGACCAGGTAGAGCATTTTATACAA---AAGACATAACAGGGGATATAAGACAA ***** ********** ********** * *** * * ******* **
S1V5-2 GCACATTGTAACATTAGTAGAGCAGACTGGAATAACACTTTAAAACAAATAGTTATGAAA S1V5-5 GCACATTGTAACATTAGTAGAGCAGACTGGAATAACACTTTAAAACAAATAGTTATGAAA S1V5-1 GCACATTGTAACATTAGTAGAGCAGACTGGAATAACACTTTAAAACAAATAGTTATGAAA S1V5-12 GCACATTGTAACATTAGTGGAACAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-13 GCACATTGTAACATTAGTGGAGCAGCATGGAATAACACTTTAAAAGAGATAGTTATGAAA S1V1-13 GCATATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V1-4 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACCTTAAAACACATAGTTATAAAA S1V2-6 GCACATTGTAACATTAGTAGAGCAAAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V2-11 GCACATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGCTATAAAA S1V1-5 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTAACAAA S1V2-5 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAGAACAGATAGTTATAAAA S1V2-12 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACGCTTTAAAACAGATAGTTATAAAA S1V1-2 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-10 GCATATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGGTATAAAA S1V1-6 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAGACAGATAGTTATAAAA S1V2-10 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-7 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-8 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-9 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-12 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V1-11 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAATCAGATAGTTATAAAA S1V1-3 GCATATTGTAACATTAGTAGAGCAGAATGGAATAACACTTTAAAACAGATAGTTATAAAA S1V2-9 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAGAACAGATAGTTATAAAA S1V2-7 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-1 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V1-1 GCATATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-14 GCATATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-15 GCATATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-4 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-8 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-13 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-16 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAGAACAGATAGTTATAAAA S1V2-2 GCTCATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V2-3 GCACATTGTAACATTAGTAGAGCAGAATGGGATAACACTTTAAAACAGATAGTTATAAAA S1V5-8 GCATATTGTAACATTAGTAGAGCAGACTGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-10 GCATATTGTAACATTAGTAGAGCAGCACGGAATAACACTTTAAAACGGATAGTTAAAAAA S1V5-11 GCATATTGTAACATTAGTAGAACAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-3 GCACATTGTAACATTAGTGGAGCAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-4 GCATATTGTAACATTAGTAGAACAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-6 GCATATTGTAACATTAGTAGAACAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-9 GCATATTGTAACATTAGTAGAACAGCATGGAATAACACTTTAAAACAGATAGTTAAAAAA S1V5-7 GCATATTGTAACATTAGTAGAGCAGCATGGGATAACACTTTAAAACGGATAGTTAAAAAA ** ************** ** ** ** ***** * *** **** ** ***
S1V5-2 TTAGGAGAACACTTGGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-5 TTAGGAGAACACTTGGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-1 TTAGGAAAACACTTGAGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-12 TTAGGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-13 TTAGGAGAACAATTTAAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-13 TTAAGAGAACACTTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-4 TTAAGAGAACACTTTGGCAACAAAACAATAGTCTTTAATCACTCTTCA S1V2-6 TTAAGAGAACACTTTGAGAATAAAACAATAGTCTTTAATCATTCCTCA S1V2-11 TTAAGAGAACACTTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-5 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-5 TTAAGAGAACGCTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-12 TTAAGAGAACACTTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-2 TTAAGAGAACACTTTAGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-10 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-6 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-10 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-7 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-8 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-9 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-12 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-11 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-3 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-9 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-7 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-1 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V1-1 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCTTCA S1V2-14 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-15 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-4 TTAAGAGAACAATTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-8 TTAGGAGAACAATTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-13 TTAAGAGAACAATTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-16 TTAAAAGAACAATTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-2 TTAAAAGAACAACTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V2-3 TTAAGAGAACAATTTGAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-8 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAACCACTCCTCA S1V5-10 TTAGGAGAACACTTTAAGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-11 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-3 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-4 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-6 TTAAGAGAACACTTTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-9 TTAAGAGAACACATTGTGAATAAAACAATAGTCTTTAATCACTCCTCA S1V5-7 TTAAGAGAACACTTTGGGAATAAAACAATAGTCTTTAATCACTCCTCA *** * *** * ** ***************** ** ** ***
Phylogenetic Tree Subject 1
Figure 3-Phylogenetic Tree-Activity 2, Part 2, Subject 1
Theta Subject 1
Subject | Number of Clones | S | Theta |
---|---|---|---|
1 | 42 | 90 | 20.8 |
Subject 3
Alignment Subject 3
S3V6-2 GATGTAGTAATCAGATCTGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V6-4 GATATAGTAATTAGATCTGCCAATTTCTCGGACAATGCTAAAACCATATTAGTACAGCTG S3V6-5 GATATAGTAATTAGATCTGCCAATTTCTCGGACAATGCTAAAACCATATTAGTACAGCTG S3V6-3 GATATAGTAATTAGATCTGCCAATTTCTCGGACAATGCTAAAACCATATTAGTACAGCTG S3V6-6 GATATAGTAATTAGATCTGCCAATTTCTCGGACAATGCTAAAACCATATTAGTACAGCTG S3V6-1 GATATAGTAATTAGATCTGCCAATTTCTCGGACAATGCTAAAACCATATTAGTACAGCTG S3V4-5 GAGGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V3-2 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATTCTAAAACCATAATAGTACAGCTG S3V3-1 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATAATAGTACAGCTG S3V1-3 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATACTAGTACAGCTG S3V1-4 GATGTAGTAATCAGATCCGCCAATTTCACGAACAATGCTAAAACCATACTAGTACAGCTG S3V5-3 GATGTAGTAATTAGATCCGCCAATTTCACAGACAATGCTAAAACCATACTAGTACAGCTG S3V4-7 GATGTAGTAATCAGATCCGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V5-2 GATGTAGTAATCAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-9 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-1 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATACTAGTACAGCTG S3V5-10 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-7 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-8 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-4 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-5 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V5-6 GATGTAGTAATTAGATCCGCCAATTTCACGAACAATGCTAAAACCATATTAGTACAGCTG S3V3-6 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATATTAGTACAGCTG S3V3-10 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATATTAGTACAGCTG S3V3-9 GATGTAGTAATTAGATCCGCCAATTTCACAGACAATGCTAAAATCATAATAGTACAGCTG S3V3-3 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATAATAGTACAGCTG S3V3-7 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATACTAGTACAGCTG S3V4-8 GATGTAGTAATTAGATCCGCCAATTTCGCGGACAATGCTAAAACCATACTAGTACAGCTG S3V1-1 GATGTAGTAATTAGATCCGCCAATTTCTCGGACAATGCTAAAACCATACTAGTACAGCTG S3V1-2 GATGTAGTAATTAGATCCGCCAATTTCTCGGACAATGCTAAAACCATACTAGTACAGCTG S3V4-2 GATGTAGTAATTAGATCCGCCAATTTCGCGGACAATGCTAAAACCATACTAGTACAGCTG S3V4-3 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V4-1 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V4-4 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAGACCATATTAGTACAGCTG S3V3-5 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATATTAGTACAGCTG S3V4-9 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAACCATATTAGTACAGCTG S3V3-8 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATATTAGTACAGCTG S3V3-4 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAATCATAATAGTACAGCTG S3V4-6 GATGTAGTAATTAGATCCGCCAATTTCACGGACAATGCTAAAACCATAATAGTACAGCTG ** ******* ***** ********* * ***** **** * **** ***********
S3V6-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V6-4 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V6-5 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V6-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V6-6 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V6-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-5 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGAGTAACT S3V3-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V1-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGAGTAACT S3V1-4 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGAGTAACT S3V5-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-7 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-9 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-10 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-7 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-8 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-4 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-5 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V5-6 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-6 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-10 AATGAAACTGTAGTAATGAATTGTACAAGACCCGACAACAATACAAGAAAAAGGGTAACT S3V3-9 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-7 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-8 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V1-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V1-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-2 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-3 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAATAATACAAGAAAAAGGGTAACT S3V4-1 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-4 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-5 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-9 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-8 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V3-4 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT S3V4-6 AATGAAACTGTAGTAATGAATTGTACAAGACCCGGCAACAATACAAGAAAAAGGGTAACT ********************************** *** ************** ******
S3V6-2 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V6-4 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V6-5 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V6-3 CTAGGACCAGGCAGAGTATACTATACCACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V6-6 CTAGGACCAGGCAGAGTATACTATACCACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V6-1 CTAGGACCAGGCAGAGTATACTATACCACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-5 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-2 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-1 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-3 CTAGGACCAGGCAAAGTATACTACACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-4 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-3 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-7 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-2 CTAGGACCGGGCAGAGTATACTATACAACAGGACAAATAATAGGGGATATAAGAAAAGCA S3V5-9 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATGAGAAAAGCA S3V5-1 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-10 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGGGATATAAGAAAAGCA S3V5-7 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-8 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-4 CTAGGACCAGGCAGAGTATACTATACCACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-5 CTAGGACCAGGCAGAGTATACTATACCACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V5-6 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-6 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-10 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-9 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-3 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-7 CTAGGACCAGGCAGAGTATACTATACAATAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-8 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-1 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V1-2 CTAGGACCAGGCAAAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-2 CTAGGACCAGGCAAAGTATATTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-3 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-1 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-4 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-5 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-9 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-8 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V3-4 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA S3V4-6 CTAGGACCAGGCAGAGTATACTATACAACAGGACAAATAATAGGAGATATAAGAAAAGCA ******** **** ****** ** ** * *************** ***** *********
S3V6-2 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V6-4 CATTGTAACCTTAGTAGAGCGGATTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V6-5 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V6-3 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V6-6 CATTGTAACCTTAGTAGAGCAGGTTGGAATAGCACTTTAGAAAGGATAGCTATAAAATTA S3V6-1 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V4-5 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAACAAGGATAGCTATAAAATTA S3V3-2 CATTGTAACCTTAGTAGAGCAGGTTGGGCTAACACTTTAGAAAGGATAGCTGTAAAATTA S3V3-1 CATTGTAACCTTAGTAGAACAGGTTGGAGTAACACTTTAAAAAGGATAGCTGTAAAATTA S3V1-3 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V1-4 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V5-3 CATTGTAACCTTAGTAGAGCGGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V4-7 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAAAAGGGATAGCTATAAAATTA S3V5-2 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V5-9 CATTGTAACCTTAGTTGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V5-1 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V5-10 CATTGTAACCTTAGTAGAGCAGGTTGAAATAACACTTTAGAAAGAATAGCTATAAAATTA S3V5-7 CATTGTAACCTTAGTAGAGCAGGTTGAAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V5-8 CATTGTAACCTTAGTAGAGCAGGTTGAAATAACACTTTAGAAAGAATAGCTATAAAATTA S3V5-4 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V5-5 CATTGTAACCTTAGTAGAGCAGGTTGGACTAACACTTTAGAAAGAATAGCTATAAAATTA S3V5-6 CATTGTAACCTTAGTAGAGCAGGTTGGACTAACACTTTAGAAAGGATAGCTATAAAATTA S3V3-6 CATTGTAACCTTAGTAGAGCAGATTGGAGTAACACTTTAGAAAGAATAGCTATAAAATTA S3V3-10 CATTGTAACCTTAGCAGAGCAGGTTGGACTAACACTTTAGAAAGAATAGCTATAAAATTA S3V3-9 CATTGTAACCTTAGTAGAGCAGGTTGGAGTAACACTTTAGAAAGGATAGCTATAAAATTA S3V3-3 CATTGTAACCTTAGTAGAGCAGGTTGGACTAACACTTTAGAAAGAATAGCTATAAAATTA S3V3-7 CATTGTAACCTTAGTAGAGCAGGTTGGACTAACACTTTAGAAAGAATAGCTATAAAATTA S3V4-8 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V1-1 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V1-2 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V4-2 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V4-3 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V4-1 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V4-4 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAGAAAGGATAGCTATAAAATTA S3V3-5 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V4-9 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V3-8 CATTGTAACCTTAGTAGAGCAGATTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V3-4 CATTGTAACCTTAGTAGAGCAAGTTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA S3V4-6 CATTGTAACCTTAGTAGAGCAGGTTGGAATAACACTTTAAAAAGGATAGCTATAAAATTA ************** ** * *** ** ******* * * ****** ********
S3V6-2 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V6-4 TGAGAACAATTTCAGAATAAAACAATAGGCTTTAATCAATCCTCA S3V6-5 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V6-3 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAACCAATCCTCA S3V6-6 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V6-1 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V4-5 AGAGAACAATTTCAGAATAGAACAATAGGCTTTAATCAATCCTCA S3V3-2 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-1 AGAGAACAATTTCAGAATAGAACAATAGGCTTTAATCAATCCTCA S3V1-3 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V1-4 AGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAATCCTCA S3V5-3 AGAGAACAATTTCAGAATAAAACAATAGGCTTTAATCAATCCTCA S3V4-7 TGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V5-2 AGAGAACAATTTCAGAATAAAACAATATTCTTTAATCAATCCTCA S3V5-9 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V5-1 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V5-10 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V5-7 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V5-8 AGAGAACAATTTCAGAATAAAACAATATTCTTTAATCAATCCTCA S3V5-4 AGAGAACAATTTCAGAATAAAACAATATTCTTTAATCAATCCTCA S3V5-5 AGAGAACAATTTCAGAATAAAACAATATTCTTTAATCAATCCTCA S3V5-6 AGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAATCCTCA S3V3-6 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-10 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-9 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-3 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-7 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V4-8 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V1-1 AGAGAACAATTTCAGAATAAAACAATAGTCTTTAATCAATCCTCA S3V1-2 AGAGAACAATTTCAGAATAAAACAATAGCCTTTAATCAATCCTCA S3V4-2 AGAGAACAATTTCAGAATAAAACAATAGGCTTTAATCAATCCTCA S3V4-3 AGAGAACAATTTCAGAATAGAACAATATTCTTTAATCAATCCTCA S3V4-1 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V4-4 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-5 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V4-9 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-8 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V3-4 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA S3V4-6 AGAGAACAATTTCAGAATAGAACAATAGTCTTTAATCAATCCTCA ****************** ******* ****** *********
Phylogenetic Tree Subject 3
Figure 4-Phylogenetic Tree-Activity 2, Part 2, Subject 3
Theta Subject 3
Subject | Number of Clones | S | Theta |
---|---|---|---|
3 | 39 | 42 | 9.88 |
Subject 5
Alignment Subject 5
S5V4-7 GAGGTAGTAATTAGATCCCAAAATCTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V4-1 GAGGTAGTAATTAGATCTCAAAATCTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V4-2 GAGGTAGTAATTAGATCTCAAAATCTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V4-6 GAGGTAGTAATTAGATCTCAAAATCTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V3-3 GAGGTAGTAATTAGATCCAAAAATTTCACGGACAATGCAAAAATCATAATAGTACATCTA S5V3-4 GAGGTAGTAATTAGATCCAAAAATTTCACTGACAATGCTAAAATCATAATAGTACATCTA S5V4-3 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V4-4 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V4-5 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V3-1 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V3-2 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V3-5 GAGGTAGTAATTAGATCCATAAATTTCTCAGACAATGCTAAAATCATAATAGTACATCTA S5V5-4 GAGGTAGTAATTAGATCCACAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V5-5 GAGGTAGTAATTAGATCCACAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V5-1 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V5-3 GAGGTAGTAATTAGATCCACAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-3 GAGGTAGTTATTAGATCCAAAAATTTCTCGGACAATGTAAAAATCATAATAGTACATCTA S5V3-6 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-6 GAGGTAGTTATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V3-8 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V3-10 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATGATAGTACATCTA S5V3-11 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATGATAGTACATCTA S5V2-10 AAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-11 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCAAAAATCATAATAGTACATCTA S5V2-5 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-1 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-9 GAAGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-2 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-12 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-7 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-8 GAGGTAGTAATTAGATCCAAAAATTTCTCGGAAAATGCTAAAATCATAATAGTACATCTA S5V1-3 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-5 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-7 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V3-7 GAGGTGGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-8 GAAGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V3-9 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-1 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAGTAGTACATCTA S5V1-6 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-4 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V2-4 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V1-2 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA S5V5-2 GAGGTAGTAATTAGATCCAAAAATTTCTCGGACAATGCTAAAATCATAATAGTACATCTA * ** ** ******** **** ** * ** **** ******** ***********
S5V4-7 AATGAATCTGTAGAAATTAATTGTACAGGACCCGGCAACAATACAAGAAGAAGTATACAT S5V4-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V4-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V4-6 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-3 AATGAATCTGTAGAAATTAATTGTACAAGACACGGCAACAACACAAGAAGAAGTATACAT S5V3-4 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAACACAAGAAGAAGTATACCT S5V4-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V4-4 AATGAATCTGTAGAAATTAATTGCATAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V4-5 AATGAATCTGTAGAAATTAATTGCACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-5 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V5-4 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V5-5 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V5-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V5-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-3 AATGAATCTGTAGAAATTAATTGCACAAGACCCGGCAACAATACAAGAAGAAGTATACCT S5V3-6 AATGAATCTGTAGAAATTAATTGCACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-6 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-8 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATAAAT S5V3-10 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-11 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-10 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-11 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACCT S5V2-5 AATGAATTTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-1 AATGAATTTGTAAAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-9 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATAAAT S5V2-2 AATGAATCTGTAGAAATTAATTGTGCAAGACCCGGCAACAATACAAGAAGAAGTATAAAT S5V2-12 AATGAATCTGTAGAAATTAATTGTGCAAGACCCGGCAACAATACAAGAAGAAGTATAAAT S5V1-7 AATGAATCTGTAGAAATTAATTGTACAAGACCCAGCAACAATACAAGAAGAAGTATACAT S5V1-8 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-3 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-5 AATGAATCTGTAGAAATTAATTGTACAAGGCCCGGCAACAATACAAGAAGAAGTATACTT S5V2-7 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACCT S5V3-7 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-8 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V3-9 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-1 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-6 AATGAGTCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-4 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V2-4 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V1-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT S5V5-2 AATGAATCTGTAGAAATTAATTGTACAAGACCCGGCAACAATACAAGAAGAAGTATACAT ***** * **** ********** * * * * ******* *************** *
S5V4-7 ATAGGGCCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V4-1 ATAGGGCCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V4-2 ATAGGGCCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCG S5V4-6 ATAGGGCCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-3 ATAGGACCAAACAGAGCATTTTATACAACAGGAGACGTAATAGGAGATATAAGACAAGCA S5V3-4 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V4-3 ATAGGACCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V4-4 ATAGGACCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V4-5 ATAGGACCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-1 ATAGGACCAAACAGAGCATTCTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-2 ATAGGACCAAACAGAGCATTCTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-5 ATAGGACCAAACAGAGCATTCTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V5-4 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V5-5 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V5-1 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V5-3 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-3 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-6 ATAGGACCAAACAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-6 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-8 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-10 ATAGGACCAGGCGGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-11 ATAGGACCAGGCGGAGCATTTTATACAACAGGAGACATAATAGGAGGTATAAGACAAGCA S5V2-10 GTAGGACCAGGCAGAGCATTTTATACAATAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-11 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-5 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-1 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-9 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-2 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-12 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-7 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGACATAAGACAAGCA S5V1-8 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGACATAAGACAAGCA S5V1-3 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGACATAAGACAAGCA S5V1-5 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-7 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-7 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-8 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V3-9 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-1 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-6 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-4 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V2-4 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V1-2 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA S5V5-2 ATAGGACCAGGCAGAGCATTTTATACAACAGGAGACATAATAGGAGATATAAGACAAGCA **** *** * ******* ******* ******* ********* ***********
S5V4-7 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V4-1 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V4-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V4-6 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V3-3 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGCTATAAAATTA S5V3-4 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGCTATAAAATTA S5V4-3 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGTTATAAAATTA S5V4-4 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGTTATAAAATTA S5V4-5 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGTTATAAAATTA S5V3-1 CATTGTAACATTAGTGAAGAAGAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V3-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V3-5 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V5-4 CATTGTAACATTAGTAAAGGAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V5-5 CATTGTAACATTAGTGAAGGAAAATGGAATGAAACCTTAAAAAAGATAGCTATAAAATTA S5V5-1 CATTGTAACATTAGTGAAGGAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V5-3 CATTGTAACATTAGTGAAGGAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V2-3 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGCTATAAAATTA S5V3-6 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-6 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGCTATAAAATTA S5V3-8 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGCTATAAAATTA S5V3-10 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V3-11 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-10 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-11 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-5 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-1 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-9 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-12 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-7 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-8 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-3 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-5 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-7 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACCTTAAAAAAGATAGTTATAAAATTA S5V3-7 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-8 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V3-9 CATTGTAATATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-1 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-6 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-4 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V2-4 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V1-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA S5V5-2 CATTGTAACATTAGTGAAGAAAAATGGAATGAAACTTTAAAAAAGATAGTTATAAAATTA ******** ****** *** * ************* ************* **********
S5V4-7 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATTCATCCTCA S5V4-1 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAACTCATCCTCA S5V4-2 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATTCATCCTCA S5V4-6 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATTCATCCTCA S5V3-3 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATCCATCCTCA S5V3-4 AGAAAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V4-3 AGAGAACAATTTAAGAATAA---AACAATAGTATTTAAGTCATCCTCA S5V4-4 AGAGAACAATTTAAGAATAA---AACAATAGTATTTAAGTCATCCTCA S5V4-5 AGAGAACAATTTAAGAATAA---AACAATAGTATTTAAGTCATCCTCA S5V3-1 AGAGAACAATTTAGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V3-2 AGAAAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V3-5 AGAGAACAATTTAGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V5-4 AGAGAACAATTTAGGAATAA---AACAATAGTCTTTAATTCATCCTCA S5V5-5 AGAGAACAATTTAGGAATAA---AACAATAGCATTTAATCCATCCTCA S5V5-1 AGAGAACAATTTAGGAATAA---AACAATAGCATTTAATTCATCCTCA S5V5-3 AGAGAACAATTTAGGAATAA---AACAATAGCATTTAATTCATCCTCA S5V2-3 AGAGAACAATTTGTGAATAA---AACAATAGTATTTAATCCATCCTCA S5V3-6 AGAAAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V2-6 AGAGAACAATTTGTGAATAA---AACAATAGTATTTAATCCATCCTCA S5V3-8 AGAGAACAATTTGTGAATAA---AACAATAGTATTTAATCCATCCTCA S5V3-10 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATTCATCCTCA S5V3-11 AGAGAACAATTTGAGAATAA---AACAATAGTATTTAATCCATCCTCA S5V2-10 AGAGAACAATTTGGGAATAAAACAACAATAGTATTTAATTCATCCTCA S5V2-11 AGAGAACAATTTGGGAATAA---AACGATAGTATTTAATTCATCCTCA S5V2-5 AGGGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V2-1 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V2-9 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V2-2 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V2-12 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V1-7 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCGCA S5V1-8 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCGCA S5V1-3 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCGCA S5V1-5 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V2-7 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V3-7 AGAAAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V2-8 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V3-9 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V1-1 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V1-6 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V1-4 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V2-4 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATTCATCCTCA S5V1-2 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA S5V5-2 AGAGAACAATTTGGGAATAA---AACAATAGTATTTAATCCATCCTCA ** ******** ****** *** **** ***** ***** **
Phylogenetic Tree Subject 5
Figure 5-Phylogenetic Tree-Activity 2, Part 2, Subject 5
Theta Subject 5
Subject | Number of Clones | S | Theta |
---|---|---|---|
5 | 43 | 48 | 11.05 |
Data and Files
Phylogenetic Trees made from Phylogeny Tree Maker [9]
Phylogenetic tree for Activity 1
Phylogenetic tree of 3 clones from Subjects 1,3,5, and 7
Phylogenetic tree of subject 1 all clones, every visit
Phylogenetic tree of subject 3 all clones, every visit
Phylogenetic tree of subject 5 all clones, every visit
Research Project
Question
Can moderate and rapid progressors be categorized in the same group?
Hypothesis
Yes, moderate and rapid progressors can be categorized together, due to the fact there was no statistical significance between the two groups.
Subjects
- Maddy and I will be performing a data clean up, we will be using 5 clones and use the first and two-year visit for each subject from the moderate and rapid progressor groups
- In our data collection we will be removing 1,4, and 7
- Subjects 4 and 7 will be removed from our data set because their starting CD4 count was very high in comparison to the other rapid and moderate progressors
- Subject 1 will be removed from our data set because they only have three data points, none of which are near the two-year mark
- We will be using five clones for each subject, for those subjects that have more than five clones we will be using a random number generator and picking five different clones from 1st and two-year visit
- Once the sequences are gathered we will be comparing them, their S and theta values and running statistics to see if there is any meaningful difference between the rapid and the moderate progressors
- In addition we will also be making a phylogenetic tree with all the clones from the first and two-year visits of the subjects chosen to see if there is any clustering present
Conclusion
In conclusion, sequencing and generating phylogenetic trees will allow one to find differences and similarities among sequences, enabling one to discover evolutionary relationships between the subjects and their clones. For Activity 2 Part 1 it was apparent that all clones from a subject clustered together, meaning they were more similar to themselves than the other subjects. For Activity 2 Part 2 Subject 1 has a higher theta value (20.8), leading one to believe there is greater diversity among clones and visits, while Subject 3 (9.88) and Subject 5 (11.05) had lower values.
Acknowledgements
- I worked with Madeleine B. King and Nathan On throughout the class and compared techniques to become more efficient in the data analysis
- I worked with Madeleine B. King twice outside of class to figure out the details in our research project
- Madeleine B. King and I went to Kam D. Dahlquist, Ph.D. to get help figuring out the details of our research project
- I copied and modified the protocol for Week 5 assignment
- I asked Kam D. Dahlquist, Ph.D. about citing protocols
- Except for what is noted above, this individual journal entry was completed by me and not copied from another source.
Mpaniag1 (talk) 13:30, 19 February 2020 (PST)
References
- Home - PubMed - NCBI. (n.d.). Retrieved February 13, 2020, from https://www.ncbi.nlm.nih.gov/pubmed
- HIV-1 subject 1 visit 1 clone 9 from USA, envelope glycoprotein V3 region (env) gene, partial cds. (n.d.). Retrieved February 13, 2020, from https://www.ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore&from_uid=9770526
- Markham, R. B., Wang, W. C., Weisstein, A. E., Wang, Z., Munoz, A., Templeton, A., ... & Yu, X. F. (1998). Patterns of HIV-1 evolution in individuals with differing rates of CD4 T cell decline. Proceedings of the National Academy of Sciences, 95(21), 12568-12573. doi: 10.1073/pnas.95.21.12568
- Nucleotide Sequences . (n.d.). Retrieved February 13, 2020, from http://bioquest.org/bedrock/problem_spaces/hiv/nucleotide_sequences.php
- Phylogeny.fr: Home. (n.d.). Retrieved February 13, 2020, from http://www.phylogeny.fr/
- OpenWetWare. (2020). BIOL368/S20:Week 5. Retrieved February 13, 2020, from https://openwetware.org/wiki/BIOL368/S20:Week_5
- The harmonic series. (n.d.). Retrieved February 13, 2020, from https://www.math.utah.edu/~carlson/teaching/calculus/harmonic.html
- Visit_1_Subjects_1_thru_9_HIV. (n.d.). Retrieved from https://s3-us-west-2.amazonaws.com/oww-files-public/9/9b/Visit_1_Subjects_1_thru_9_HIV.txt
- Visit_1_Subjects_10_thru_15_HIV. (n.d.). Retrieved February 13, 2020, from https://s3-us-west-2.amazonaws.com/oww-files-public/4/4c/Visit_1_Subjects_10_thru_15_HIV.txt
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