BME100 f2017:Projects5

BME 100 Fall 2017	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
Lab 5: “PCR and Fluorescence Detection of DNA” Content from PCR Lab C and Lab D PCR Reactions Procedure Data Analysis HW: Lab Report 5 (due 11/07/17 at 11:59 pm through OpenWetWare) Grading Scheme: 10 points – Overall layout: Our Team section, grammar, style 10 points - Lab C content - PCR reaction report 10 points – Lab D content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter 20 points – Lab D content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary 50 points – Peer Assessment Rubric     EXCELLENT ACCEPTABLE WEAK* OVERALL LAYOUT OUR TEAM section 5 pts = Original & non-offensive images; real names 3 pts = More than half original images & information 1 pt = All default green & white avatars; default text Grammar & style 5 pts = Composition & layout perfect throughout 3 pts = Few spelling errors; images too big or small 1 pt = Many errors PCR REACTION REPORT Summary 10 pts = Complete description, good text formatting 6 pts = Poor composition 2 pts = Missing (only default text) FLUORIMETER PROCEDURE Webcam set-up 5 pts = Clear, easy to reproduce based on writing 3 pts = Somewhat ambiguous 2 pts = Only one sentence Placing samples onto the fluorimeter 5 pts = Steps are thorough, clear, and easy to follow 3 pts = Run-on paragraphs 1 pt = Instructions are vague/ incomplete DATA COLLECTION & ANALYSIS Callibration: 3 drop images, data table, 2 plots with best fit lines 10 pts = Complete 6 pts = Missing one part, somewhat unorganized 2 pts = Missing two or more parts, very unorganized EXTRA CREDIT: gel electrophoresis +5 pts = Image of the gel; text labels on image; typed list corresponding to gel (what is in each lane) +3 pts = Image of the gel; missing labels or list +1 pts = Image of the gel, no labels, no sample list PCR results: 2 drop images, data table, final results 10 pts = Complete 6 pts = Missing one part, somewhat unorganized 2 pts = Missing two or more parts, very unorganized Note: *Empty/ plagiarized sections or borrowed images without references receive zero points Important Information About Grading: Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited. Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline. Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT! If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button. This should open up an Edit page that is pre-populated with a lab report template code. Group1_W0800_L5 Group2_W0800_L5 Group3_W0800_L5 Group4_W0800_L5 Group5_W0800_L5 Group6_W0800_L5 Group7_W0800_L5 Group8_W0800_L5 Group9_W0800_L5 Group10_W0800_L5 Group11_W0800_L5 Group12_W0800_L5 Group13_W0800_L5 Group14_W0800_L5 Group15_W0800_L5 Group16_W0800_L5 Group17_W0800_L5   Group1_W1030_L5 Group2_W1030_L5 Group3_W1030_L5 Group4_W1030_L5 Group5_W1030_L5 Group6_W1030_L5 Group7_W1030_L5 Group8_W1030_L5 Group9_W1030_L5 Group10_W1030_L5 Group11_W1030_L5 Group12_W1030_L5 Group13_W1030_L5 Group14_W1030_L5 Group15_W1030_L5 Group16_W1030_L5 Group17_W1030_L5