User:Korey Griffin
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Contact
- Korey Griffin
- Email me through OpenWetWare
Replicating genetic information on earth manifests a vast fingerprint of potentiating life. Life sciences research advances knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Conditioned Medium Preparation (Growth Factor/Cytokine)
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Empirical Resources
- Pubmed
- Entrez Gene
- Tissue Expression Profiling through Human Protein Atlas
- Allen Murine Brain Atlas
- Brain Atlas In Situ RNA Hybridization
- ATCC Cell Lines
- Organelle and Lineage Specific Markers
- DOT guidelines
- Fluorochrome Table
- Oligo Tm
- Technical Support Portal UCLA
Prokaryotic Systems
- Prokaryotic (bacteria + archaea) Phage Defense
- CRISPR On Target Off Target Dynamics
- Bacteria Nomenclature g+ (monoderm)/g- (polyderm)
- Commensal Ortholog Cross-Reactivity
Immunology
Genomics
Molecular
Cell Nomenclature
Scientific Epistemology
- Principia Scientific International
- Climate Science
- Glaciation Science
- Pleistocene (Quaternary) Glacitation
- Bolide Event Atlas
- NIH Director Francis Collins Interview
- Grand solar minima and maxima
- Late Holocene / Anthropocene Epoch
- Northern Hemisphere forcing of climatic cycles in Antarctica over the past 360,000 years
- Eight glacial cycles from an Antarctic ice core
- Model evidence for a seasonal bias in Antarctic ice cores
- Milankovitch Cycles theory
