User:Korey Griffin
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Contact
- Korey Griffin
- SCBT Inc.
- Email me through OpenWetWare
Replicating genetic information on planet earth manifests an extraordinary fingerprint of potentiating life. Collective intelligence through our Holocene epoch prescribes value to empiricism. One example being research paradigms within scientific methods.
Research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Conditioned Medium Preparation (Growth Factor/Cytokine)
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Empirical Resources
- Brain Atlas In Situ RNA Hybridization
- ATCC Cell Lines
- Organelle and Lineage Specific Markers
- DOT guidelines
- Fluorochrome Table
- Human Protein Atlas
- Oligo Tm
- Tissue Expression Profiling through Human Protein Atlas
- Single Cell Sequencing
