User contributions
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Jump to navigationJump to search- 13:44, 15 August 2007 diff hist +687 Jessica Karen Wong/Notebook/2007-8-14 current
- 13:16, 15 August 2007 diff hist +25 User:Jessica Karen Wong/Progress →I2056 current
- 13:12, 15 August 2007 diff hist +377 N Jessica Karen Wong/Notebook/2007-8-14 New page: *Minipreped and sent for sequencing **E0240-ES (E2-3, E2-4, M1-4, M1-3, M1-2) **I2056-SX (4,8) **I2056-EX (2,8) **I2055-EX (2,3) **I2055-SX (4,6) *PCR cleaned and heatshocked I2055 and I20...
- 13:07, 15 August 2007 diff hist 0 N File:8-13.jpg current
- 13:05, 15 August 2007 diff hist +61 Jessica Karen Wong/Notebook/2007-8-13 current
- 13:05, 15 August 2007 diff hist +707 N Jessica Karen Wong/Notebook/2007-8-13 New page: *Ran gel of the overnight I2056, I2055 colony PCR **Top Row: I56EX (1-8) sp lad sp I56SX (1-8) **Bottom Row:I55SX (1-8) sp lad sp I56SX (9) I56EX (9) I55SX (9) I55EX (1-5) *Made overnight...
- 14:08, 13 August 2007 diff hist +23 User:Jessica Karen Wong/Progress
- 14:04, 13 August 2007 diff hist +5 User:Jessica Karen Wong/Progress →E0240
- 13:58, 13 August 2007 diff hist 0 N File:8-12.jpg current
- 13:57, 13 August 2007 diff hist +473 N Jessica Karen Wong/Notebook/2007-8-12 New page: thumb|left|Dpn1 digest of I2055 and I2056 *PCR cleaned I2055 and I2056's dpn1 digests of PCR prod **Ran a gel loaded: lad I2056-EX I2056-SX I2055-EX I2055-SX **Still had... current
- 13:52, 13 August 2007 diff hist 0 N File:8-11.jpg current
- 13:51, 13 August 2007 diff hist +509 N Jessica Karen Wong/Notebook/2007-8-11 New page: thumb|left| I2055/I2056 BB PCR Gel *Ran a gel of I2055 and I2056 BB redo PCR **Loaded: Lad I2055-EX I2055-SX I2056-EX I2056-SX **Digested the PCR w/ Dpn1 overnight *Min... current
- 13:45, 13 August 2007 diff hist +20 User:Jessica Karen Wong/Progress →I2055
- 13:44, 13 August 2007 diff hist +26 User:Jessica Karen Wong/Progress
- 12:54, 13 August 2007 diff hist -62 User:Jessica Karen Wong/Progress →I2056
- 12:52, 13 August 2007 diff hist +64 User:Jessica Karen Wong/Progress
- 12:35, 13 August 2007 diff hist +61 Jessica Karen Wong/Notebook/2007-8-10 current
- 12:35, 13 August 2007 diff hist +442 Jessica Karen Wong/Notebook/2007-8-10
- 12:15, 13 August 2007 diff hist 0 N File:8-10.jpg current
- 12:14, 13 August 2007 diff hist +1,063 N Jessica Karen Wong/Notebook/2007-8-10 New page: *Minipreped overnights *Sent for seq - I2056-ENX (3,5,7,8) I2056-SNX (1,5,6,8) I2055-SX-B34 (2 colonies) *Transformation plates looked better (after leaving them to rest for a day) *Made o...
- 10:25, 13 August 2007 diff hist 0 Jessica Karen Wong/Notebook/2007-8-6 current
- 10:14, 13 August 2007 diff hist +1,034 Jessica Karen Wong/Notebook/2007-8-9 current
- 13:30, 10 August 2007 diff hist -1 Jessica Karen Wong/Notebook/2007-8-9
- 13:29, 10 August 2007 diff hist 0 N File:8-9.jpg current
- 13:29, 10 August 2007 diff hist +336 Jessica Karen Wong/Notebook/2007-8-9
- 10:48, 10 August 2007 diff hist -35 User:Jessica Karen Wong/Progress →I2057
- 14:35, 9 August 2007 diff hist +470 N Jessica Karen Wong/Notebook/2007-8-9 New page: *All overnight plates (I2056's and I2055's from yesterday's ligations) have a good amount of growth, very little fluorescence though **However, last time they got a lot more red after bein...
- 13:55, 9 August 2007 diff hist +23 Jessica Karen Wong/Notebook/2007-8-8 current
- 13:47, 9 August 2007 diff hist +2 Jessica Karen Wong/Notebook/2007-8-8
- 13:47, 9 August 2007 diff hist +344 Jessica Karen Wong/Notebook/2007-8-8
- 13:40, 9 August 2007 diff hist 0 Jessica Karen Wong/Notebook/2007-8-8
- 13:32, 9 August 2007 diff hist 0 N File:8-8.jpg current
- 13:29, 9 August 2007 diff hist +1,009 N Jessica Karen Wong/Notebook/2007-8-8 New page: *Minipreped 2 samples of I2055-SX-B0030 for seq **Sent both samples (1 was fluor and 1 wasn't) for seq w/ VF & VR *PCR cleaned I2056 digests (E/X, S/X, both N) *Ligated I2056-ENX closed, I...
- 11:02, 9 August 2007 diff hist 0 N File:08-7.jpg current
- 11:01, 9 August 2007 diff hist +1 Jessica Karen Wong/Notebook/2007-8-7 current
- 11:01, 9 August 2007 diff hist +47 Jessica Karen Wong/Notebook/2007-8-7
- 11:00, 9 August 2007 diff hist +859 N Jessica Karen Wong/Notebook/2007-8-7 New page: *Ran a gel of the overnight I2056 BB pcr's **Loaded: Lad EX SX **Both look right size *Heat Shocked I2055 S/X digest from PCR *Minipreped E0240-EX, E0240-ES, F2620 for seq *PCR cleaned I20...
- 13:31, 8 August 2007 diff hist -35 User:Jessica Karen Wong/Progress →I2056
- 13:10, 8 August 2007 diff hist +77 User:Jessica Karen Wong/Progress →I2055
- 13:04, 8 August 2007 diff hist +9 User:Jessica Karen Wong/Progress →I2057
- 13:04, 8 August 2007 diff hist +63 User:Jessica Karen Wong/Progress →I2057
- 13:01, 8 August 2007 diff hist +23 User:Jessica Karen Wong/Progress →E0240
- 13:00, 8 August 2007 diff hist +134 User:Jessica Karen Wong/Progress →T9002
- 14:50, 6 August 2007 diff hist +14 Jessica Karen Wong/Notebook/2007-8-6
- 14:50, 6 August 2007 diff hist +2 Jessica Karen Wong/Notebook/2007-8-6
- 14:49, 6 August 2007 diff hist +327 Jessica Karen Wong/Notebook/2007-8-6
- 14:05, 6 August 2007 diff hist 0 N File:8-6-small.jpg current
- 14:05, 6 August 2007 diff hist 0 N File:8-6-large.jpg current
- 14:05, 6 August 2007 diff hist +89 Jessica Karen Wong/Notebook/2007-8-6
- 14:01, 6 August 2007 diff hist +1,334 N Jessica Karen Wong/Notebook/2007-8-6 New page: *Ran a gel of overnight pcr's *large gel: **Row 1 : E0240 M1 ES (4-1), E0240 M2 ES (1-4), SP LAD SP E0240-ES-E2 (4-1)E0240-EX-E2 (1-4) **Row 2 : F2620-3K3-1 (4-1), F2620-3K3-2 (1-4), SP ...
