Jessica Karen Wong/Notebook/2007-8-14

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  • Minipreped and sent for sequencing
    • E0240-ES (E2-3, E2-4, M1-4, M1-3, M1-2)
    • I2056-SX (4,8)
    • I2056-EX (2,8)
    • I2055-EX (2,3)
    • I2055-SX (4,6)
  • PCR cleaned and heatshocked I2055 and I2056 EX SX Not1 digests
  • Sealed (ligated) I2055-ENX, I2055-SNX, I2056-ENX, I2056-SNX
    • Transformed in Top10 and plated
  • Overnighted liquid cultures of I2055-EX-B34, I2055-SX-B31, I2055-SX-B34


Sequencing

  • I2055-ENX-2 : fuzzy read, not all of xba site is there, end looks good
  • I2055-EX-B0032 : GFP isn't all there
  • I2056-7 Re-re-confirmed that it's scarred and built
  • I2057-EX : PERFECT!
  • I2055-SX-B34-1 Most likely right all there and covered except for 87-130
    • 2nd sample doesn't contain b34
  • I2056-EX-8 perfect, completely covered except for 35-200
    • 7 looks good too but not fully covered
    • 5 has a 1bp mutation
    • 3 doesn't have all of R0040
  • I2056-SX-1 PERFECT!
    • 5 has a 1bp mutation
    • 6 is most likely good, stops around 650 of RFP
    • 8 is also PERFECT!
  • F2620 : both 4 and 6 failed
  • I2055-EX-B32 just had p1010, bad
  • I2055-SX-B32 failed
  • I2057-EX-J116 had not b0032